Trimeric autotransporter adhesins in Acinetobacter baumannii, coincidental evolution at work

Rahbar, Mohammad Reza; Zarei, Mahboubeh; Jahangiri, Abolfazl; Khalili, Saeed; Nezafat, Navid; Negahdaripour, Manica; Fattahian, Yaser; Ghasemi, Younes

doi:10.1016/j.meegid.2019.03.023

Cited by 24 publications

(14 citation statements)

References 78 publications

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“…Paralogs of different sequence lengths are common within the species, which is indicative of duplication events; this conclusion is also supported by the evolutionary analyzes (see the evolutionary analysis). However, in comparison with Gammaproteobacterial TAAs, such as Acinetobacter species TAAs (Rahbar et al, 2019), the length of the sequences is shorter and less heterogeneous.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that due to the TAA sequence diversity, alignment and tree-based analyzes are not suitable to investigate the properties of TAAs (Pina et al, 2009;Fialho and Mil-Homens, 2011). Hence, all-against-all BLAST was harnessed here, as an appropriate alternative to handle the massive data, including very disparate sequences (Rahbar et al, 2019). Furthermore, it is difficult to annotate the domains of TAAs due to their considerable sequence diversity, the various patterns of domain arrangements, and the limited coverage of sequences by known structures (Meng et al, 2006;Bassler et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…A 1333 amino acid sequence was the most abundant sequence in the population (see Supplementary Data 2, CLANS tab, for details of different clusters). In general, the sequence lengths of BTAAs are heterogeneous [compare it with Acinetobacter, which is highly heterogenous (Rahbar et al, 2019)]. BatA is a member of the cluster 1.…”

Section: Clustering the Btaasmentioning

confidence: 99%

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Non-adaptive Evolution of Trimeric Autotransporters in Brucellaceae

et al. 2020

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Brucella species are Gram-negative, facultative intracellular pathogens. They are the main cause of brucellosis, which has led to a global health burden. Adherence of the pathogen to the host cells is the first step in the infection process. The bacteria can adhere to various biotic and abiotic surfaces using their outer membrane proteins. Trimeric autotransporter adhesins (TAAs) are modular homotrimers of various length and domain complexity. They are a diverse, and widespread gene family constituting the type Vc secretion pathway. These adhesins have been established as virulence factors in Brucellaceae . To date, no comprehensive and exhaustive study has been performed on the trimeric autotransporter family in the genus. In the present study, various bioinformatics tools were used to provide a novel evolutionary insight into the sequence and structure of this protein family in Brucellaceae . To this end, a dataset of all trimeric autotransporters from the Brucella genomes was built. Analyses included but were not limited to sequence alignment, phylogenetic tree constructions, codon-based test for selection, clustering of the sequences, and structure (primary to quaternary) predictions. Batch analyzes of the dataset suggested the existence of a few structural domains within the whole population. BatA from the B. abortus 2308 genome was selected as a reference to describe the features of these structural domains. Furthermore, we examined the structural basis for the observed rigidity and resiliency of the protein structure through a molecular dynamics evaluation, which led us to deduce that the random drift results in the non-adaptive evolution of the trimeric autotransporter genes in the Brucella genus. Notably, the modifications have occurred across the genus without interference of gene transmission.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Non-adaptive Evolution of Trimeric Autotransporters in Brucellaceae

et al. 2020

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“…In vivo, mechanisms of the evolutionary underlying the development of this adhesin is not clear. The Ata is an useful multi-functional virulence agent in the bacterium Acinetobacter baumannii that assists the invasion and the adhesion, participates with pathogenicity, and incites apoptosis [60]. It was found that the Ata is acting as a multi-functional virulence agent of Acinetobacter baumannii by (1) mediating the invasion and adhesion in cells of epithelial and endothelial, (2) leading to the programmed cell death in a caspas-dependent manner, (3) leading to the secreting IL-6 and IL-8 as proinflammatory cytokines, and (4) in vivo, contributes to the virulence.…”

Section: Acinetobacter Trimeric Autotransporter (Ata)mentioning

confidence: 99%

Virulence and Antibiotic Resistance of Acinetobacter baumannii among Urinary Tract Infections

Al-Dahmoshi¹,

Al-Khafaji²,

Al-Alaq³

2022

Urinary Tract Infection and Nephropathy - Insights Into Potential Relationship

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Acinetobacter baumannii is one of the opportunistic bacteria firstly related with the hospital acquired infection influencing primarily to weakening the patient in the ICU. It is sometimes transferred to the patient by transient colonization of hands of the workers of healthcare, and persistence on eco-surfaces. Acinetobacter baumannii inhalation aerosolized through endo-tracheal suctioning of the ventilated patient is widespread among ventilator-related pneumonia (VAP). It is infections mainly associated with ventilator-related pneumonia (VAP), community Acquired Pneumonia (CAP), invasive bacterial infections (IBIs) and UTI (urinary tract infection). It is one of the prominent uropathogens problematic with antibiotic resistance especially carbapenem resistant Acinetobacter baumannii (CRAB). Their colonization of urinary tract and establishment of infection may attributed mainly to set of virulence factors like: Acinetobactin-assisted iron acquisition system, Bap (biofilm-related protein), phospholipase D, Ata (Acinetobacter trimeric autotransporter), chaperone-usher type pilus (Csu), OmpA (outer membrane protein A), and Plasminogen-binding protein (CipA). The common drugs used for treatment Acinetobacter baumannii infections involve polymyxins, glycylcyclines, tetracyclines, mono-bactams, fluoroquinolones, aminoglycosides, antipseudomonal carbapenems, antipseudomonal cephalosporins, and sulbactam. The rates of MDR isolation or also comprehensively the resistant Acinetobacter baumannii are significantly increased and so the combination of two or more (colistin, tigecycline, or colistin-rifampicin combination therapy) drugs is sometimes used to treat infections of MDR-AB. As a conclusion the Acinetobacter baumannii engagement in urinary tract infections attributed mainly to their adhesins, invasins and intrinsic antibiotic resistance.

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“…Membrane anchoring region could be used for demarcating the family, thanks to its conservancy across all TAAs (Bassler et al 2015;Rahbar et al 2019). Therefore, in the present study, the transmembrane region was served as a query for extracting available sequence data InterPro (Finn et al 2017) database by querying the YadA like membrane anchoring domain (IPR005594).…”

Section: Clustering the Taas Of Moraxellaceae Familymentioning

confidence: 99%

Pierce into the Native Structure of Ata, a Trimeric Autotransporter of Acinetobacter baumannii ATCC 17978

Rahbar

Zarei

Jahangiri

et al. 2019

Int J Pept Res Ther

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Acinetobacter baumannii is an important pathogen responsible for nosocomial infections worldwide. Trimeric autotransporters, the obligate homotrimeric adhesins, are involved in adherence of bacterial cells to various surfaces. These sorts of adhesins were shown to be expressed by A. baumannii. Trimeric autotransporters are modular virulence factors, containing numerous domains and structural architectures. The better understanding of the sequence and structural features of virulence factors are crucial in designing new therapeutic strategies. In this regard, with the aid of reliable in silico tools and the concept of "inherence through homology", some sequence and structural features of Ata A.baumannii were unveiled. Domain architectures such as the position of repetitive modules, and coiled-coils, along with the prediction of tertiary and quaternary structures, allows us to define some important landscapes of Ata A.baumannii virulence factor. In addition, through CLANS analysis of TAA sequences of Moraxellaceae family, it was concluded that Ata contains several conserved of structural blocks of TAAs. The protein is initiated with extended signal peptide region, a stalk of several head domains and a membrane anchoring region. The globular heads are connected to each other by neck mediators and coiled-coil regions. Several fibronectin and collagen binding sites were defined within the structures. Minimal and maximal frustrated contacts are distributed within the structure of Ata, which suggest both the flexibility and toughness. These are come together for creating an efficient adhesin which is able to bind and bend through multiple sites. The protein could be great target for designing new vaccines or anti-virulence drug.

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Trimeric autotransporter adhesins in Acinetobacter baumannii, coincidental evolution at work

Cited by 24 publications

References 78 publications

Non-adaptive Evolution of Trimeric Autotransporters in Brucellaceae

Non-adaptive Evolution of Trimeric Autotransporters in Brucellaceae

Virulence and Antibiotic Resistance of Acinetobacter baumannii among Urinary Tract Infections

Pierce into the Native Structure of Ata, a Trimeric Autotransporter of Acinetobacter baumannii ATCC 17978

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