Trimethoprim Resistance by Class I Integron in Vibrio parahaemolyticus from a Fish Farm

Sixty-seven Vibrio parahaemolyticus isolates from surface seawater from the Wando area, on the southern coast of Korea, were analyzed for their susceptibility to 15 different antimicrobials and the presence of virulence genes. According to the disk diffusion susceptibility test, all of the strains studied were resistant to ampicillin and oxacillin, while decreasing percentages were resistant to vancomycin (64.2%), streptomycin (56.7%), amikacin (31.3%), kanamycin (22.3%), cephalothin (20.9%), erythromycin (10.4%), ciprofloxacin (4.5%), and tetracycline (3.0%). All of the strains were susceptible to five antimicrobials: chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazole/ trimethoprim, and trimethoprim. Fifty-nine isolates (88.1%) were resistant to three or more classes of antimicrobial and defined as multidrug resistant, and two strains were resistant to seven antimicrobial agents. The minimum inhibitory concentration (MIC) of the 67 V. parahaemolyticus isolates to ampicillin and oxacillin ranged from 512−2,048 and 64−512 µg/mL, respectively. All 67 isolates were also examined for the presence of the tdh and trh virulence genes using the polymerase chain reaction (PCR). However, no isolates possessed either tdh or trh. The VPA0477 (β-lactamase) gene, present in all of the tested strains, was validated as a new specific marker gene in PCR assays for the accurate detection and identification of V. parahaemolyticus.

Section: 서 론 (Vibrio Parahaemolyticus)mentioning

confidence: 99%

Antimicrobial-resistance Profiles and Virulence Genes of Vibrio parahaemolyticus Isolated from Seawater in the Wando Area

김태옥¹,

엄인선²,

조상만³

et al. 2014

“…, . (Son et al, 2005;Lee et al, 2007;Oh et al, 2008;Lee et al, 2009;Ryu et al, 2010;Yu et al, 2010;Kim et al, 2014;Kang et al, 2016;Kim et al, 2016). , , .…”

unclassified

Antimicrobial Resistance and Minimum Inhibitory Concentrations of Vibrio parahaemolyticus Strains Isolated from Gomso Bay, Korea

Kim¹,

Um²,

Kim³

et al. 2016

Seventy-nine Vibrio parahaemolyticus isolates from surface seawater from Gomso Bay, west coast of Korea, were analyzed for the presence of virulence genes and their susceptibility to 30 different antimicrobials. All 79 isolates were examined for the presence of two virulence genes (tdh or trh) using polymerase chain reaction (PCR); however, no isolates possessed either the tdh or trh gene. According to a disk diffusion susceptibility test, all of the strains studied were resistant to oxacillin, penicillin, and vancomycin, followed by ticarcillin (97.5%), ampicillin (96.2%), clindamycin (86.1%), erythromycin (10.1%), streptomycin (7.6%), cefoxitin (6.3%), amikacin (2.5%), and cephalothin (2.5%). However, all of the strains were susceptible to 19 other antimicrobials including cefepime, cefotaxime, chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazole/trimethoprim, and trimethoprim. All 79 isolates (100%) were resistant to four or more classes of antimicrobials, and two strains exhibited resistance to eight antimicrobial agents. The average minimum inhibitory concentrations (MICs) for V. parahaemolyticus for ampicillin, penicillin, ticarcillin, and vacomycin were 946.5, 1,305.9, 1,032.3, and 45.0 µg/mL, respectively.

“…수산용으로 사용된 전체 항생제의 사용량은 2005 년 한해 275톤을 정점으로 서서히 감소추세이나 2008년도 사용량은 194톤으로 아직도 항생제 사용량은 많은 편이며, 다량의 항생제 사용은 각종 항생제 내성균의 증가를 가중시키 는 결과로 나타나고 있다 (Son et al, 2003;Son et al, 2005;Lee et al, 2007;Oh et al, 2008;Lee et al, 2009;Yu et al, 2010 (Makino et al, 2003). Trimethoprim에 내성을 갖는 장염비브리오는 classs I integron 내에 존재하는 dfrA27 유전자 에 의해 trimethoprim, gentamicin 및 rifampin의 내성에 관여하 는 유전자가 포함되어 있다는 연구결과가 최근에 보고하였다 (Yu et al, 2010 (Lee et al, 2007;Lee et al, 2009). 이러한 결과는 우리나라에만 국한되고 있는 것이 아니라 외국의 예도 유사한 실정이다 (Tanil et al, 2005;Baker-Austin et al, 2008;Ferrini et al, 2008 (Fig.…”

unclassified

Antibiotic-Resistance Profiles and the Identification of the Ampicillin-Resistance Gene of Vibrio parahaemolyticus Isolated from Seawater

Lee¹,

Park

2010

The antibiotics-resistance profiles of 28 strains of Vibrio parahaemolyticus isolated from seawater were investigated. All of the strains studied were resistant to ampicillin (100%), but susceptible to 12 other antibiotics. The minimum inhibitory concentration (MIC) of V. parahaemolyticus to ampicillin was as high as 1,024-2,048 μg·mL -1 . The phenotype of strain 8 changed from ampicillin-resistant to susceptible with an in-frame deletion mutant of VPA0477, a putative β-lactamase gene, and the MIC for ampicillin of the mutant strain was 1 μg·mL -1 . In conclusion, our findings suggest that the VPA0477 gene acts as a β-lactamase in ampicillin-resistant V. parahaemolyticus strains.