Trimethylselenonium hydroxide: A new methylating agent

Yamauchi, Kanae

doi:10.1016/s0040-4039(01)94661-1

Cited by 4 publications

(2 citation statements)

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“…Products resulting from the lipase-catalyzed hydrolysis of borage oil were fractionated into a water phase that contained FFA and an organic phase that contained acylglycerols (TG, DG, and MG) by adding 0.1 mL of 1 N NaOH in a 50% ethanol solution. Acylglycerols were collected by evaporating organics in nitrogen atmosphere (22). To convert acylglycerols into fatty acid methyl esters (FAME), 5 mg of acylglycerol, a known amount of internal standard (heptadecanoic acid dissolved in 50 µL of 1,2dichloroethane), and 50 µL of 0.2 M TMSH (trimethylsulfonium hydroxide)-methanol solution were placed in a test tube; the tube was shaken by vortex for approximately 20 s and then put aside for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Enrichment of γ‐linolenic acid from borage oil via lipase‐catalyzed reactions

Huang

1997

J Americ Oil Chem Soc

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Three lipase-catalyzed reactions were utilized to enrich γ-linolenic acid in borage oil: (i) selective hydrolysis in isooctane by Candida rugosa lipase immobilized on microporous polypropylene, (ii) selective esterification of free fatty acid from saponified borage oil and n-butanol by Lipozyme IM-20, and (iii) acidolysis of the products of the previous two reactions, that is, unhydrolyzed acylglycerols and unesterified free fatty acid. In the selective hydrolysis, γ-linolenic acid content could be raised from 23.6 mol% in borage oil to 51.7% in the unhydrolyzed acylglycerols. On the other hand, γ-linolenic acid content in free fatty acid could be increased to 87% after selective esterification. Products with 65% γ-linolenic acid in their acylglycerols were obtained by means of the acidolysis reaction. JAOCS 74, 977-981

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Section: Methodsmentioning

confidence: 99%

Enrichment of γ‐linolenic acid from borage oil via lipase‐catalyzed reactions

Huang

1997

J Americ Oil Chem Soc

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“…Fatty acid compositions in acylglycerols by gas chromatography (GC) analysis (15). GC was employed for the determination of fatty acid compositions of the acidolysis reaction.…”

Section: Methodsmentioning

confidence: 99%

γ‐Linolenic acid‐rich triacylglycerols derived from borage oil via lipase‐catalyzed reactions

Huang

Chiang

1999

J Americ Oil Chem Soc

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γ-Linolenic acid (GLA), a precursor of arachidonic acid, possesses physiological functions of modulating immune and inflammatory response. Highly purified GLA is desired both as a medicine and as an ingredient of cosmetics. In this work, urea fractionation and lipase-catalyzed reactions were employed for the enrichment of GLA in borage oil. GLA content in free fatty acids from saponified borage oil can be increased from 23.6 to 94% by the method of urea fractionation. Partial hydrolysis of borage oil catalyzed by immobilized Candida rugosa lipase raises GLA content in the unhydrolyzed acylglycerols from 23.6 to 52.1%. The IM-60 catalyzed acidolysis reaction between the GLA-rich free fatty acid and the unhydrolyzed acylglycerols increases the GLA content in the acylglycerols from 52.1 to 75%. The acylglycerols in the reaction product contains ca. 90% triacylglycerol. The effects of temperature, water content, substrate weight ratio, and organic solvents on the GLA content in the acylglycerols were examined.Paper no. J8985 in JAOCS 76, 833-837 (July 1999).

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