Triplet state population study in relation to the aggregation of proflavine in frozen solutions

Lion, Yves; Saucin, Michel; Vorst, A. Van de

doi:10.1007/bf01323421

Cited by 5 publications

(1 citation statement)

References 10 publications

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“…The irradiation (30 s) was performed directly in the spectrometer cavity as described by Lion et al (1975). The total profiavine concentrations used to photosensitize the three substrates ranged from 0.02--0.5 mM.…”

Section: Irradiation and Epr Measurementsmentioning

confidence: 99%

Influence of the DNA structure on the free radical induction due to proflavine and light treatment

Piette

Calberg-Bacq

Vorst

1979

Radiat Environ Biophys

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Induction of peroxide free radicals (detected by Electron Paramagnetic Resonance at 77 K) due to the photodynamic activity of proflavine was measured on bacteriophage phi X174 DNA either single-stranded (ss) as isolated from the virion, or double-stranded supercoiled (RFI) as isolated from the infected bacteria. Comparison was made with calf thymus DNA photosensitization. In order to use equivalent DNA-proflavine complexes, binding of the dye to the three DNA's was first determined under those conditions of high ionic strength favourable to the photodynamic reaction. Free radical induction was maximal for definite amounts of bound proflavine (which varied depending upon the DNA substrate) and at an ionic strength value of 0.5. The level of the maximal reaction increased in the following order: from phi Xss DNA to calf thymus DNA and finally to phi XRFI DNA. The conformation of the proflavine-DNA complex was thus a determinant for the efficiency of the photodynamic process. The ionic strength effect could not be explained by the evolution of the proflavine triplet state in irradiated proflavine-calf thymus DNA complexes.

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Section: Irradiation and Epr Measurementsmentioning

confidence: 99%