Tris–<scp>EDTA</scp>significantly enhances antibiotic efficacy against multidrug‐resistant<i><scp>P</scp>seudomonas aeruginosa in vitro</i>

Buckley, Laura; McEwan, Neil; Nuttall, Tim

doi:10.1111/vde.12071

Cited by 37 publications

(32 citation statements)

References 45 publications

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“…Proteus mirabilis clinical isolates also were four to eight times more susceptible to disodium EDTA than P. aeruginosa clinical isolates. In our study, the MIC range for Tris‐EDTA against canine otic Pseudomonas isolates was comparatively lower (1,500/470–3,000/930) than those reported previously, with the range of 2,200/600–8,900/2,400 μg/mL. The antimicrobial activity of disodium EDTA against both Gram‐positive and Gram‐negative ATCC strains in our study was quite close to the findings in a previous study …”

Section: Discussionsupporting

confidence: 87%

“…Infections with these organisms are typically treated with antimicrobial drugs, but there are increasing concerns about bacterial resistance and the requirement for diligent antimicrobial stewardship . Meticillin‐resistant S. pseudintermedius (MRSP), which is often multidrug‐resistant, as well as multidrug‐resistant P. aeruginosa strains are frequently isolated from canine OE and/or skin infections. Alarmingly, studies have shown that antimicrobial resistance in the human population, especially in pet owners and veterinarians, can be associated with organisms of canine origin .…”

Section: Introductionmentioning

confidence: 99%

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In vitroantimicrobial activity of seven adjuvants against common pathogens associated with canine otitis externa

Chan

Khazandi

Hickey

et al. 2018

Veterinary Dermatology

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Background -An antibiotic adjuvant is a chemical substance used to modify or augment the effectiveness of primary antimicrobial agents against drug-resistant micro-organisms. Its use provides an alternative approach to address the global issue of antimicrobial resistance and enhance antimicrobial stewardship.Hypothesis/Objectives -To determine the antimicrobial activity of a panel of potential antimicrobial adjuvants against common pathogens associated with canine otitis externa (OE).Animals/Isolates -A number of type strains and clinical isolates (n = 110) from canine OE were tested including Staphylococcus pseudintermedius, b-haemolytic Streptococcus spp., Pseudomonas aeruginosa, Proteus mirabilis and Malassezia pachydermatis.Methods and materials -Antimicrobial activities of monolaurin, monocaprin, N-acetylcysteine (NAC), polymyxin B nonapeptide, Tris-EDTA, Tris-HCL and disodium EDTA were tested using microdilution methodology according to CLSI guidelines.Results -N-acetylcysteine, Tris-EDTA and disodium EDTA had antimicrobial activity against both type strains and otic pathogens. The other adjuvants tested had limited to no efficacy. NAC had a minimal inhibitory concentration (MIC) range of 2,500-10,000 lg/mL for the various organisms. Pseudomonas aeruginosa isolates were eight times more susceptible to disodium EDTA in the presence of Tris-HCL in comparison to disodium EDTA alone. Malassezia pachydermatis isolates were most susceptible to Tris-EDTA (MIC 90 = 190/60 lg/mL) and disodium EDTA (MIC 90 = 120 lg/mL).Conclusions and clinical relevance -N-acetylcysteine, Tris-EDTA and disodium EDTA have intrinsic antimicrobial activity and represent promising adjuvants that could be used to enhance the efficacy of existing antibiotics against Gram-negative and multidrug-resistant bacterial infections. These agents could be combined with other antimicrobial agents in a multimodal approach for mixed ear infections in dogs.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

In vitroantimicrobial activity of seven adjuvants against common pathogens associated with canine otitis externa

Chan

Khazandi

Hickey

et al. 2018

Veterinary Dermatology

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“…This is because, EDTA can combine with the magnesium ions from the lipopolysaccharides (LPS) of the cell envelopes, resulting in LPS release and increased permeability of the cell membrane, facilitating infiltration of antibiotics into the cells [11]. Clinically, EDTA may also potentially change the response of bacteria in multiple-drug-resistant bacterial strains [28].…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo activity of EDTA and antibacterial agents against the biofilm of mucoid Pseudomonas aeruginosa

Liu

Lin

et al. 2016

Infection

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“…21 Although Tris-EDTA alone is known to be bacteriostatic, its addition to topical products significantly lowers the MIC and MBC values for several antimicrobials. 22 Antimicrobial peptides could represent a promising alternative to traditional antimicrobial drugs. A synergistic antimicrobial effect for AMP2041 with chlorhexidine has been hypothesized, due to their similar modes of action.…”

Section: Discussionmentioning

confidence: 99%

In vitro antimicrobial activity of a gel containing antimicrobial peptide AMP2041, chlorhexidine digluconate and Tris‐EDTA on clinical isolates of Pseudomonas aeruginosa from canine otitis

Ghibaudo¹,

Santospirito

Sala

et al. 2016

Veterinary Dermatology

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Background -Pseudomonas aeruginosa (PA) may cause suppurative otitis externa with severe inflammation and ulceration in dogs. Multidrug resistance is commonly reported for this organism, creating a difficult therapeutic challenge.Objective -The aim of this study was to evaluate the in vitro antimicrobial activity of a gel containing 0.5 lg/mL of antimicrobial peptide AMP2041, 0.07% chlorhexidine digluconate (CLX), 0.4% Tris and 0.1% EDTA on 30 clinical isolates of PA from canine otitis externa.Materials and Methods -Antimicrobial activity was evaluated through minimal bactericidal concentration (MBC). Standardized bacterial suspensions were incubated with different concentrations of the gel at 37°C for 30 min and plated for colony forming unit (CFU) counts. Time-to-kill kinetics were evaluated with the undiluted product and at MBC for each PA strain at 30 s, 1, 5, 10, 15, 30 min, 24 and 48 h.Results -The MBC was 1:64 for two of 30 strains, 1:128 for 15 of 30 strains and 1:256 for 13 of 30 strains. The geometric mean was 1:165, equivalent to a concentration of 0.003 lg/mL AMP2041 + 0.0004% CLX + 0.0024% Tris + 0.0006% EDTA. Time-to-kill assays with the undiluted product showed complete bactericidal effect within 30 s for all isolates, whereas at the MBC this effect was reached within 5 min for 20 of 30 isolates and within 30 min for all isolates. Bactericidal activity was maintained after 48 h for all isolates.Conclusion -This gel has shown rapid, complete and long-lasting activity against a panel of 30 PA isolates from cases of canine otitis externa.

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Tris–EDTAsignificantly enhances antibiotic efficacy against multidrug‐resistantPseudomonas aeruginosa in vitro

Abstract: Tris-EDTA significantly reduced the MBCs and MICs of marbofloxacin and gentamicin for multidrug-resistant P. aeruginosa in vitro. This may be of use to clinicians managing these infections in dogs.

Cited by 37 publications

References 45 publications

In vitroantimicrobial activity of seven adjuvants against common pathogens associated with canine otitis externa

In vitroantimicrobial activity of seven adjuvants against common pathogens associated with canine otitis externa

In vitro and in vivo activity of EDTA and antibacterial agents against the biofilm of mucoid Pseudomonas aeruginosa

In vitro antimicrobial activity of a gel containing antimicrobial peptide AMP2041, chlorhexidine digluconate and Tris‐EDTA on clinical isolates of Pseudomonas aeruginosa from canine otitis

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