Tritium labelling of antidepressants with regard to their chemical structure

Buchman, Ouri; Azran, Jacques; Shimoni, M.

doi:10.1002/jlcr.2580200713

Cited by 6 publications

(2 citation statements)

References 12 publications

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“…Solvent Systems for chromatography: 51 = 2-propanol : benzene (l 4-1) 5 2 = acetone : ammonia (99 + 1) 5 3 = methanol: ammonia (100 + 1.5) 5 4 = ethyl acetate : n-propanol: ammonia (40 + 30 + 3) 5 5 = methanol : ammonia (100 + 2) 5 6 = Chloroform : ethanol: ammonia (12 + 8 + 0.1) 5 7 = methanol : ammonia (95 + 5) 5 8 = benzene : methanol: ammonia (90 + 15 + 1)…”

Section: Introductionmentioning

confidence: 99%

“…Immunogens for dibenzazepines and dibenzcycloheptanodienes were prepared using desmethylderivatives of imipramine and amitriptyline. After conversion into hemisuccinates and conjugation with bovine serum albumin immunization of rabbits was carried out according to Hubbard et al 3 (5,6). The solvent and excess bromine were removed by lyophilization and the residue was dissolved in doxane.…”

Section: Introductionmentioning

confidence: 99%

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Radioimmunoassay of Dibenzazepines and Dibenzcycloheptanodienes in Body Fluids and Tissues

Krulík

Exner²,

Fuksová³

et al. 1991

Clinical Chemistry and Laboratory Medicine

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Sumraary: In an attempt to establish a radioimmunoassay (RIA), imipramine and amitriptyline immunogens were prepared; desmethyl derivatives were converted into hemisuccinates, conjugated with bovine serum albumin and used for rabbit immunization. [ 3 H]Amitriptyline (4.3 TBq/mmol) and [ 3 H]imipramine (2.9 TBq/mmol) were prepared by catalytic dehalogenation or reductive alkylation. Dibenzazepines and dibenzcycloheptanodienes were determined in biological fluids by a direct method without deproteinization (lower detection limit of 0.5 μg • l" 1); using high-yield methods they were extracted from cell membranes. Assay of tricyclic antidepressants in humans showed that these substances disappear from plasma much earlier than from cell membranes. Dissociation of the antidepressants bound to cell membranes *is slow and their plasma concentrations are not influenced by Standing for 2 h at 4 °C. During preparing the membranes for binding studies these substances are not removed, and they may affect the results of the binding studies.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Radioimmunoassay of Dibenzazepines and Dibenzcycloheptanodienes in Body Fluids and Tissues

Krulík

Exner²,

Fuksová³

et al. 1991

Clinical Chemistry and Laboratory Medicine

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synthesis of 10‐[³H]‐5H‐dibenz[b, f] azepine‐5‐carboxamide ([³H]‐carbamazepine)

Brundish

1985

Labelled Comp Radiopharmac

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Tritium labeling of simple 7‐membered ring compounds

Hiltunen

Peng

Yang

1990

Labelled Comp Radiopharmac

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Seven-membered ring compounds, from cylcoheptane to complex ring structures containing heteroatoms, substituents and fused phenyl rings, were labeled with tritium, using activated and adsorbed tritium. The 7-membered ring structures are generally stable towards reactions with tritium, which allows compounds like 1-benzosuberone, 1-am-2-methoxy-1cycloheptene, iminostilbene and clozapine to be labeled to reasonably high specific activities. The best method varies greatly from compound to compound. By optimizing the labeling conditions and use of efficient support exceptionally good results can be obtained. The Pd-on-alumina support gives consistently higher specific activity and less radioimpurity than other supports. Even molecules containing carbon-halogen bond and hydrogen bound to nitrogen can usually be labeled with tritium at stable positions and without dehalogenation.

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Tritium labelling of antidepressants with regard to their chemical structure

Abstract: compounds a r e compared. The r e s u l t s a r e discussed by comparison w i t h hydroaendeuterium exchange and w i t h regard t o the chemical s t r u c t u r e o f these d e r i v a t i v e s .

Cited by 6 publications

References 12 publications

Radioimmunoassay of Dibenzazepines and Dibenzcycloheptanodienes in Body Fluids and Tissues

Radioimmunoassay of Dibenzazepines and Dibenzcycloheptanodienes in Body Fluids and Tissues

synthesis of 10‐[³H]‐5H‐dibenz[b, f] azepine‐5‐carboxamide ([³H]‐carbamazepine)

Tritium labeling of simple 7‐membered ring compounds

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Tritium labelling of antidepressants with regard to their chemical structure

Abstract: compounds a r e compared. The r e s u l t s a r e discussed by comparison w i t h hydroaendeuterium exchange and w i t h regard t o the chemical s t r u c t u r e o f these d e r i v a t i v e s .

Cited by 6 publications

References 12 publications

Radioimmunoassay of Dibenzazepines and Dibenzcycloheptanodienes in Body Fluids and Tissues

Radioimmunoassay of Dibenzazepines and Dibenzcycloheptanodienes in Body Fluids and Tissues

synthesis of 10‐[3H]‐5H‐dibenz[b, f] azepine‐5‐carboxamide ([3H]‐carbamazepine)

Tritium labeling of simple 7‐membered ring compounds

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synthesis of 10‐[³H]‐5H‐dibenz[b, f] azepine‐5‐carboxamide ([³H]‐carbamazepine)