Tropane alkaloid production by hairy roots of Atropa belladonna obtained after transformation with Agrobacterium rhizogenes 15834 and Agrobacterium tumefaciens containing rol A , B , C genes only

Bonhomme, Valérie; Laurain-Mattar, Dominique; Lacoux, Jérôme; Fliniaux, Marc‐André; Jacquin-Dubreuil, Annie

doi:10.1016/s0168-1656(00)00287-x

Cited by 114 publications

(54 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This time has been greatly reduced as compared to Horsch et al 20) who submerged leaf disks overnight in the bacterial culture. This time has been greatly increased as compared to Bonhomme et al 21) who submerged leaf disks 5 min in the bacterial suspension. The co-culture times were 0.…”

Section: Optimization Of the Agrobacterium Infection Processmentioning

confidence: 99%

Salidroside Production by Hairy Roots of Rhodiola sachalinensis Obtained after Transformation with Agrobacterium rhizogenes

Zhou

Wang

et al. 2007

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Agrobacterium rhizogenes is responsible for the formation of adventitious roots known as "hairy roots". Hairy roots result from the transfer and integration of the genes located on the root-inducing plasmid Ri of Agrobacterium rhizogenes into the plant genome and their expression therein.1) These roots are characterized by an extensive growth, associated with lateral branching, leading to an important mass of adventitious roots exhibiting a typical phenotype. Furthermore, these roots are found to produce a high yield of secondary metabolites as compared to that of undifferentiated plant cell suspensions.2-4) The greatest advantage of hairy roots is that hairy root cultures often exhibit about the same or greater biosynthetic capacity for secondary metabolite production compared to their mother plants. Hairy root cultures have been shown to accumulate many valuable secondary metabolites. For example, several laboratories have shown that hairy roots can produce artemisinin, tropane alkaloid, and so on. [5][6][7][8] Another advantage of hairy roots is that hairy root system is genetic stable compared to the roots of a whole plant. 9) Recently, many medicinal plants have been transformed successfully by A. rhizogenes and the hairy roots induced show a relatively high productivity of secondary metabolites, which are potentially important pharmaceutical products.10-13) Rhodiola sachalinensis A. BOR, a perennial herb, belonging to the family Crassulaceae, is mainly distributed in mountains at the altitudes of 1700-2500 m. It is a typical alpine plant and a very important medicinal plant with high activities of anti-fatigue, anti-senescence, and anti-radiation, due to the secondary metabolite salidroside, one of the major compounds from the roots of Rhodiola species (Crassulaceae). 14)Since the natural supply of this herb is rapidly decreasing, plant cell suspension culture has been considered an alternative of producing salidroside. Although there are some production of salidroside in the plant cell suspension culture of Rhodiola sachalinensis, 15) the biggest challenge of producing secondary metabolites from the suspension cultures is that secondary metabolites are usually produced by specialized cells differentiated at distinct developmental stages.16,17) To produce Salidroside more efficiently and stably, we established the hairy root system of Rhodiola sachalinensis for high yield salidroside production with A. rhizogenes. The results have demonstrated that salidroside production in most cases of the system is higher than in normal field-grown plants. This paper firstly reports the production of transformed roots using A. rhizogenes strain A4 and regeneration hairy roots from such transformants. The stable transformation of transgenes has been shown by polymerase chain reaction (PCR) and PCR-Southern blotting. As far as it is known, this is the first time that salidroside production by hairy roots of Rhodiola sachalinensis obtained after transformation with Agrobacterium rhizogenes. MATERIALS AND METHODS Bacterial ...

show abstract

Section: Optimization Of the Agrobacterium Infection Processmentioning

confidence: 99%

Salidroside Production by Hairy Roots of Rhodiola sachalinensis Obtained after Transformation with Agrobacterium rhizogenes

Zhou

Wang

et al. 2007

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…Contrasting results were observed in case of the hairy root cultures of Hyoscyamus albus, 18 Atropa belladonna 19 and Coleus forskohlii 20 where the WP medium exhibited positive influence on the final biomass yields. The influence of different basal media formulations on the secondary metabolite productivities of hairy root cultures had also been noted earlier in case of Trachelium caeruleum 21 and Polygonum tinctorium.…”

mentioning

confidence: 63%

Yield enhancement strategies for the production of picroliv from hairy root culture ofPicrorhiza kurroaRoyle ex Benth.

Verma

Singh

Negi

et al. 2015

Plant Signaling & Behavior

View full text Add to dashboard Cite

Fast-growing hairy root cultures of Picrorhiza kurroa induced by Agrobacterium rhizogenes offers a potential production system for iridoid glycosides. In present study we have investigated the effects of various nutrient medium formulations viz B5, MS, WP and NN, and sucrose concentrations (1-8%) on the biomass and glycoside production of selected clone (14-P) of P. kurroa hairy root. Full strength B5 medium was found to be most suitable for maximum biomass yield on the 40th day of culture (GI D 32.72 § 0.44) followed by the NN medium of the same strength (GI D 22.9 § 0.43). Secondary metabolite production was 1.1 and 1.3 times higher in half strength B5 medium respectively in comparison to MS medium. Maximum biomass accumulation along with the maximum picroliv content was achieved with 4% sucrose concentration in basal medium. RT vitamin and Thiamine-HCl effected the growth and secondary metabolite production of hairy roots growing on MS medium but did not show any effect on other media. The pH of the medium played significant role in growth and secondary metabolite production and was found to be highest at pH 6.0 while lowest at pH 3.0 and pH 8.0. To enhance the production of biomass and Picroliv 5 liter working capacity bioreactor was used, 27-fold (324 g FW) higher growth was observed in bioreactor than shake flask and secondary metabolite production was similarly enhanced.

show abstract

“…The isolated DNA was analysed by PCR using rolA [33], rolB [34], rolC [13,28], rolD [35], and TR-DNA [36] specific primers. To eliminate the chances of false positive PCR products due to bacterial contamination, Vir D1-specific primers were used [37].…”

Section: Analyses Of T-dna Genes In Ri-transformed Cultures Atmentioning

confidence: 99%

The Fate of Integrated Ri T-DNA rol Genes during Regeneration via Somatic Embryogenesis in Tylophora indica

2015

View full text Add to dashboard Cite

The fate of integrated Ri T-DNA rol genes during regeneration via indirect somatic embryogenesis and stability of its effect on morphology and tylophorine content of Ri-transformed plants have been studied in Tylophora indica. Integration and expression of Ri T-DNA genes in transformed embryogenic callus lines derived from transformed root lines, 300 Ri-transformed somatic embryos, and 23 Ri-transformed plant lines were analysed. Fifty root lines studied showed integration and expression of four rol genes of TL-DNA. Spontaneous regeneration via indirect somatic embryogenesis was obtained from root lines that were TL + /TR − . Stable integration and expression of rol genes were observed in root lines, embryogenic callus lines, and the spontaneously induced somatic embryos. Nineteen out of the 23 Ri-transformed plant lines and their clones showed phenotypic and genetic stability over the period of 3 years. Four Ri-transformed plants were morphologically similar to nontransformed plants but showed variation with the integration and expression of the rolA gene and absence of other rol genes. Variant Ri-transformed plant line A 4 28#1-V showed highest tylophorine content (2.93 ± 0.03 mg gDW −1 ) among plant lines studied. The effects of T-DNA genes on growth, morphology, and tylophorine content of the Ri-transformed plants were stable in the long term culture.

show abstract

Tropane alkaloid production by hairy roots of Atropa belladonna obtained after transformation with Agrobacterium rhizogenes 15834 and Agrobacterium tumefaciens containing rol A , B , C genes only

Cited by 114 publications

References 15 publications

Salidroside Production by Hairy Roots of Rhodiola sachalinensis Obtained after Transformation with Agrobacterium rhizogenes

Salidroside Production by Hairy Roots of Rhodiola sachalinensis Obtained after Transformation with Agrobacterium rhizogenes

Yield enhancement strategies for the production of picroliv from hairy root culture ofPicrorhiza kurroaRoyle ex Benth.

The Fate of Integrated Ri T-DNA rol Genes during Regeneration via Somatic Embryogenesis in Tylophora indica

Contact Info

Product

Resources

About