Trophoblast Differentiation: An In Vitro Model for Trophoblast Giant Cell Development

Peters, Tim J.; Bm, Chapman; Soares, Mario J.

doi:10.1385/1-59259-066-7:301

Cited by 20 publications

(46 citation statements)

References 41 publications

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“…The Rcho-1 trophoblast cell line was derived from a rat choriocarcinoma and is capable of differentiating along the trophoblast giant cell lineage (Faria & Soares 1991, Peters et al 2000. Cells were routinely plated at a density of 2-2·5 10 5 cells per 75 cm 2 flask in RPMI-1640 culture medium supplemented with 20% fetal bovine serum (FBS), 50 µM 2-mercaptoethanol, 1 mM sodium pyruvate, 100 U/ml penicillin, and 100 µg/ml streptomycin at the initiation of each experiment.…”

Section: Cell Culture Modelsmentioning

confidence: 99%

Migratory trophoblast cells express a newly identified member of the prolactin gene family

Wiemers¹,

Ain²,

Ohboshi³

et al. 2003

Journal of Endocrinology

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Rodents possess an expanded prolactin (PRL) family of genes. These genes encode for a family of structurally related hormones/cytokines that are expressed most prominently in the anterior pituitary, uterus and placenta. In this study, we have identified a new member of the rat PRL family through a search of the National Center for Biotechnology Information expressed sequence database. The cDNA was sequenced and its corresponding mRNA characterized. On the basis of existing nomenclature, the rat cDNA was termed PRL-like protein-N (PLP-N). PLP-N has structural features indicative of its inclusion in the PRL family and is most closely related to PRL-like protein-F (PLP-F) and proliferin related protein (PLF-RP). A survey of PLP-N mRNA expression by Northern analysis indicated that PLP-N showed extensive expression in the metrial gland and minimal expression in the chorioallantoic placenta or other tissues. Expression of PLP-N mRNA was restricted to migratory trophoblast cells. Junctional zone trophoblast cells isolated from day 13 of gestation placenta differentiated in vitro and exhibited a capacity for PLP-N expression. In summary, we have discovered a new member of the PRL family that is prominently expressed in migratory trophoblast cells residing in the metrial gland.

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Section: Cell Culture Modelsmentioning

confidence: 99%

Migratory trophoblast cells express a newly identified member of the prolactin gene family

Wiemers¹,

Ain²,

Ohboshi³

et al. 2003

Journal of Endocrinology

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“…The expression of differentiation specific markers is restricted to morphologically distinct giant cells. The only apparent difference observed is the failure of PL-I expression to terminate during the course of the culture as it does in vivo [13,16,17]. These cells have thus proven to be a valuable tool for studying the biology of trophoblast giant cells and offer a good model for elucidating the effect of high glucose concentration on different markers of trophoblast giant cell differentiation.…”

Section: Effect Of High Glucose On Pl-i and Pl-ii Expressionmentioning

confidence: 99%

“…The Rcho-1 trophoblast cell line can be maintained in a proliferative state or induced to differentiate into trophoblast giant cells that morphologically and functionally resemble trophoblast giant cells developing in situ [13,14].…”

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confidence: 99%

Effect of high concentrations of glucose on differentiation of rat trophoblast cells in vitro

et al. 2003

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Aims/hypothesis. Previous studies have shown that diabetic placentas are characterized by structural and biochemical anomalies, including defects in the differentiation of trophoblasts. In this study, the Rcho-1 cell line was used to investigate the impact of high glucose concentrations on different markers of differentiation of rat trophoblast cells in giant cells (endoreduplication, invasive phenotype and endocrine phenotype). Materials. Rcho-1 cells were incubated for 12 days in medium supplemented with different concentrations of glucose and 10% horse serum to optimize differentiation. The cells were examined for the proportion of nuclei showing signs of apoptosis. The effect of high glucose was investigated on the endoreduplication process, on invasive phenotype (secretion of gelatinase B) and on endocrine phenotype (expression of placental lactogen I (PL-I) and II (PL-II) and progesterone secretion). Results. Apoptosis was not induced by high glucose in Rcho-1. The number of cells was higher in the cultures exposed to high glucose (p<0.05) and their nuclei contained more DNA compared with control cells (p<0.001), while their nuclear size was smaller (p<0.001). Gelatinase B secretion increased during differentiation but no difference was found when gelatinase B secretion from trophoblasts exposed to high glucose was compared with the control cells. Rcho-1 cell cultures showed an increase in PL-I and PL-II mRNA expressions during differentiation and which was not affected by high glucose. Progesterone secretion increased during differentiation in control cultures. However, this increase was abolished when trophoblasts were cultured in high glucose. Conclusions/interpretation. Our data suggest that high glucose influences the endoreduplication process and the steroidogenesis during differentiation of rat trophoblasts. [Diabetologia (2003) 46:276-283]

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“…These cells were induced to differentiate to resemble trophoblast giant cells [15,[22][23][24]. The morphological differences between undifferentiated and differentiated cells are clearly shown in fig 1. While the stem cells appear as angular shaped cells (fig 1A), the differentiated cells exhibit large nuclei with multiple nucleoli and a highly vesicularized cytoplasm ( fig 1B).…”

Section: Cathepsin P Expression Is Induced During Differentiation Of mentioning

confidence: 97%

Expression of Cathepsin P mRNA, Protein and Activity in the Rat Choriocarcinoma Cell Line, Rcho-1, During Giant Cell Transformation

Hassanein

Korant

et al. 2007

Placenta

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Lysosomal proteases perform critical functions in protein turnover and are essential for normal growth and development. Cathepsin P is a member of a newly discovered family of lysosomal cysteine proteases uniquely expressed in rodent placenta (PECs), and is closely related to human cathepsin L. Using the rat choriocarcinoma cell line model, Rcho-1, mRNA for the PECs cathepsins P, M, Q, R, 1, 2 was found to increase in expression during differentiation into a trophoblast giant cell phenotype. By contrast, expression of cathepsin L was not regulated. A specific enzyme assay was developed to show that activity of cathepsin P mirrored mRNA expression during differentiation. Cathepsin P protein co-localizes with cathepsin B, indicating that the enzyme probably functions in the endosomal-lysosomal compartment. This study demonstrates that the PEC genes produce functional proteases that can perform specific placental roles that are probably performed by broader specificity proteases in human placenta.

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Trophoblast Differentiation: An In Vitro Model for Trophoblast Giant Cell Development

Cited by 20 publications

References 41 publications

Migratory trophoblast cells express a newly identified member of the prolactin gene family

Migratory trophoblast cells express a newly identified member of the prolactin gene family

Effect of high concentrations of glucose on differentiation of rat trophoblast cells in vitro

Expression of Cathepsin P mRNA, Protein and Activity in the Rat Choriocarcinoma Cell Line, Rcho-1, During Giant Cell Transformation

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