Trophoblastic Interferons: Do they Modulate Uterine Cellular Markers at the Time of Conceptus Attachment in the Pig?

Cencič, Avrelija; Guillomot, Michel; Koren, Simon; Bonnardière, Claude La

doi:10.1016/s0143-4004(03)00135-8

Cited by 71 publications

(82 citation statements)

References 43 publications

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“…However, the majority of ISGs are induced or increased in response to the conceptus or IFNτ only in the endometrial stroma and middle to deep GE of the ovine uterus [37,41,42,44,45]. Interestingly, the induction of many ISGs is also observed in the porcine uterus during early pregnancy, and their expression is limited to the endometrial stroma [46][47][48]. In the ovine uterus, the lack of ISG induction in the endometrial LE and sGE by IFNτ is apparently due to the expression of IFN regulatory factor two (IRF-2), a potent repressor of gene transcription that is constitutively expressed in the endometrial LE and sGE and increased during early pregnancy [44].…”

Section: Pregnancy Recognition Signaling By Interferon Tau (Ifnτ)mentioning

confidence: 99%

“…The "induced" conceptus death results from ansynchrony between development of the conceptus and uterine environment [111]. Available results indicate that estrogens of blastocyst origin are essential for maternal recognition of pregnancy in pigs and that secretory proteins from the conceptus, including interferons, play other roles during early pregnancy in pigs, including cellular changes that lead to increased polarity of endometrial epithelial cells for exocrine secretion of components of histotroph, including PGF [47,96,122]. Figure 3 Schematic illustrating the inhibition of luteolysis in pigs.…”

Section: Endocrine-exocrine Theory Of Pregnancy Recognition In Pigsmentioning

confidence: 99%

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Conceptus signals for establishment and maintenance of pregnancy

Spencer

2004

Animal Reproduction Science

164

202

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Establishment and maintenance of pregnancy results from signaling by the conceptus (embryo/fetus and associated extraembryonic membranes) and requires progesterone produced by the corpus luteum (CL). In most mammals, hormones produced by the trophoblast maintain progesterone production by acting directly or indirectly to maintain the CL. In domestic animals (ruminants and pigs), hormones from the trophoblast are antiluteolytic in that they act on the endometrium to prevent uterine release of luteolytic prostaglandin F2 alpha (PGF). In cyclic and pregnant sheep, progesterone negatively autoregulates expression of the progesterone receptor (PR) gene in the endometrial luminal (LE) and superficial glandular epithelium (GE). Available evidence in cyclic sheep indicates that loss of the PR is closely followed by increases in epithelial estrogen receptors (ER) and then oxytocin receptors (OTR), allowing oxytocin to induce uterine release of luteolytic PGF pulses. In pregnant sheep, the conceptus trophoblast produces interferon tau (IFN tau) that acts on the endometrium to inhibit transcription of the ER alpha gene directly and the OTR gene indirectly to abrogate development of the endometrial luteolytic mechanism. Subsequently, sequential, overlapping actions of progesterone, IFN tau, placental lactogen (PL) and growth hormone (GH) comprise a hormonal servomechanism that regulates endometrial gland morphogenesis and terminal differentiated function to maintain pregnancy in sheep. In pigs, the conceptus trophoblast produces estrogen that alters the direction of PGF secretion from an endocrine to exocrine direction, thereby sequestering luteolytic PGF within the uterine lumen. Conceptus estrogen also increases expression of fibroblast growth factor 7 (FGF-7) in the endometrial LE that, in turn, stimulates proliferation and differentiated functions of the trophectoderm, which expresses the FGF-7 receptor. Strategic manipulation of these physiological mechanisms can offer therapeutic schemes to improve uterine capacity, conceptus survival and reproductive health.

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Section: Pregnancy Recognition Signaling By Interferon Tau (Ifnτ)mentioning

confidence: 99%

Section: Endocrine-exocrine Theory Of Pregnancy Recognition In Pigsmentioning

confidence: 99%

Conceptus signals for establishment and maintenance of pregnancy

Spencer

2004

Animal Reproduction Science

164

202

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“…Attachment stage porcine blastocysts are heterogeneous in shape and in the onset of trophoblastic production of estrogen and IFNs ␦ and ␥ (14). These trophoblastic IFNs are proposed to play important roles during attachment of conceptuses to the uterus (15). The most rapidly maturing (elongating), earliest attaching blastocysts appear to create a hostile environment within the endometrial secretions for their more slowly developing, genetically normal littermates and appear to outcompete them for survival.…”

mentioning

confidence: 99%

Differential Gene Expression in Endometrium, Endometrial Lymphocytes, and Trophoblasts during Successful and Abortive Embryo Implantation

Tayade

Black

Yuan

et al. 2006

The Journal of Immunology

113

134

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Prenatal mortality reaching 30% occurs during the first weeks of gestation in commercial swine. Mechanisms for this are unknown although poor uterine blood supply has been postulated. In other species, vascular endothelial growth factor, hypoxia-inducible factor 1-α, and IFN-γ regulate gestational endometrial angiogenesis. Vascular endothelial growth factor and hypoxia-inducible factor 1-α are also important for placental angiogenesis while trophoblastic expression of Fas ligand is thought to protect conceptuses against immune-mediated pregnancy loss. In this study, we document dynamic, peri-implantation differences in transcription of genes for angiogenesis, cytokine production, and apoptosis regulation in the endometrium, and laser capture microdissected endometrial lymphocytes and trophoblasts associated with healthy or viable but arresting porcine fetuses. In healthy implantation sites, endometrial gene expression levels differed between anatomic subregions and endometrial lymphocytes showed much greater transcription of angiogenic genes than trophoblasts. In arresting fetal sites, uterine lymphocytes had no angiogenic gene transcription and showed rapid elevation in transcription of proinflammatory cytokines Fas and Fas ligand while trophoblasts showed elevated transcription of IFN-γ and Fas. This model of experimentally accessible spontaneous fetal loss, involving blocked maternal angiogenesis, should prove valuable for further investigations of peri-implantation failure of normally conceived and surgically transferred embryos in many species, including the human.

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“…Reports are available on trophoblastic interferons in other species (Ezashi et al, 2008;Cencic et al, 2003). The trophoblastic-interferons secreted in non-ruminants, during the peri-implantation period of pregnancy, are not IFNt (Mirando et al, 1990).…”

Section: Resultsmentioning

confidence: 99%

HOMOLOGY MODELING OF BUFFALO (<i>BUBALUS BUBALIS</i>) INTERFERON-TAU PROTEIN

Joshi¹

2013

American Journal of Bioinformatics

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Interferon-Tau (IFNt) contributes towards maternal recognition of pregnancy in ruminants (like, cattle, buffalo, goat, giraffe). IFNt has been extensively studied in most of the ruminants except for buffalo Bubalus bubalis). The present study has been undertaken to predict the secondary structure of Interferon-tau in buffalo. The available amino acid sequence of bubaline IFNt (sequence database of SwissProt) was subjected to protein-BLAST to find similar sequences with high scores and low e-values. The ovine IFNt sequence (PDB code: 1B5L) was selected for further computational analysis of the bubaline IFNt sequence to predict the secondary and tertiary structure. The secondary structure of the modeled bubaline IFNt was predicted using STRIDE. The 3D structure was generated using academic version of MODELER9v6. The probability density functions (pdf) were used to restrain Cα-Cα distances, main chain N-O distances as well as main-chain and side-chain dihedral angles. The energy minimization and van der waal contacts were taken care of using ACCELRYS DS Modeling 2.0. The residue profiles of the obtained three-dimensional models were checked by VERIFY3D. The energetic architecture and the correctness of the generated model revealed that the predicted secondary model was correct and acceptable. The predicted structure of the bubaline IFNt constructed through homology modeling from ovine IFNt, could be used for further profiling the species specific difference in IFNt activity.

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Trophoblastic Interferons: Do they Modulate Uterine Cellular Markers at the Time of Conceptus Attachment in the Pig?

Cited by 71 publications

References 43 publications

Conceptus signals for establishment and maintenance of pregnancy

Conceptus signals for establishment and maintenance of pregnancy

Differential Gene Expression in Endometrium, Endometrial Lymphocytes, and Trophoblasts during Successful and Abortive Embryo Implantation

HOMOLOGY MODELING OF BUFFALO (<i>BUBALUS BUBALIS</i>) INTERFERON-TAU PROTEIN

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