Trueperella pecoris sp. nov. isolated from bovine and porcine specimens

Abstract

A novel Gram-stain-positive bacterium was isolated from a purulent bovine milk sample, the bovine placenta from an abortion, the udder secretion of a heifer and the lung of a pig that had succumbed from suppurative bronchopneumonia in Switzerland from 2015 to 2019. The strains grew best under aerobic conditions with 5 % CO2 and colonies were non-haemolytic and greyish-white. They were non-motile and negative for catalase and oxidase. The genomes of the four strains 19M2397T, 15A0121, 15IMD0307 and 19OD0592 were obtained by sequencing. The results of phylogenetic analyses based on the 16S rRNA gene grouped them within the genus Trueperella in the family Arcanobacteriaceae . The genomes had DNA G+C contents of 61.2–62.2 mol% and showed digital DNA–DNA hybridization (dDDH) values of 21.4–22.8 % and average nucleotide identity (ANI) values of approximately 77 % to their closest relatives Trueperella pyogenes and Trueperella bernardiae . With respect to the presence in different livestock species we propose the name Trueperella pecoris sp. nov. The type strain is 19M2397T (=CCOS 1952T=DSM 111392T), isolated from the udder secretion of a heifer diagnosed with summer mastitis in 2019.

Phenotypic and genotypic approach to characterize a Trueperella pecoris strain isolated from necrotic vestibulitis of a camel (Camelus dromedarius)

“…However, Schönecker et al. (2021) stated a weak CAMP reaction with T. pecoris isolated from cows and a pig. The results of the commercial API‐Coryne identification test system revealed positive reactions for pyrrolidonyl arylamidase, β‐glucuronidase and α‐glucosidase and negative reactions for nitrate reduction and pyrazinamidase, alkaline phosphatase, β‐galactosidase and N ‐acetyl‐β‐glucosaminidase.…”

“…The biochemical profile of T. pecoris in the study by Schönecker et al. (2021) was carried out by VITEK 2 (ANC card for identification of anaerobic and coryneform bacteria). However, some results obtained by VITEK 2 supported the findings of the present study obtained with the API‐Coryne test system.…”

“…Nonetheless, according to Schönecker et al. (2021), all four T. pecoris strains were clustered together on a branch closest to T. pyogenes using MALDI‐TOF MS.…”

“…MZ467052) were compared with recently identified type strain T. pecoris DSM 111392 T (MW114829) (Schönecker et al . 2021), T. pyogenes DSM 20630 T (X79225), T. abortisuis DSM 19515 T (FN667628), T. bernardiae DSM 9152 T (HE653979) and Arcanobacterium haemolyticum DSM 20595 T (AJ234059). The partially sequenced 16S rRNA gene of strain T. pecoris 203/7 compared with the type strain T. pecoris DSM 111392 T showed an identity of 99.9%.…”

“…More recently, a novel species was assigned to the genus Trueperella , namely T. pecoris (Schönecker et al . 2021). The T. pecoris strains were isolated in Switzerland between 2015 and 2019 from a purulent bovine milk sample, from the bovine placenta after abortion, from the udder secretion of a heifer and from the lung of a pig that had succumbed to suppurative bronchopneumonia.…”