Trueperella pyogenes and Escherichia coli as an etiological factor of endometritis in cows and the susceptibility of these bacteria to selected antibiotics

Brodzki, Piotr; Bochniarz, Mariola; Brodzki, Adam; Wrona, Z.; Wawron, W.

doi:10.2478/pjvs-2014-0096

Cited by 24 publications

(27 citation statements)

References 34 publications

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“…Brodzki et al [ 22 ] found that E. coli isolated from bovine uteri was sensitive to amoxicillin-clavulanic acid by the percentage of 100% supporting the results of high sensitivity to this antibiotic. On the contrary, Li-ming et al [ 23 ] found that E. coli was highly resistant to amoxicillin.…”

Section: Discussionmentioning

confidence: 89%

Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles

Raheel¹,

Hassan²,

Salem³

et al. 2020

J Adv Vet Anim Res

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Objective: The objectives of this study were to determine the biofilm-forming capability and antimicrobial susceptibility of Escherichia coli recovered from bovine endometritis samples. Materials and Methods: A total of 120 uterine specimens were collected from cows suffering from endometritis for bacteriological examination. Antimicrobial susceptibility testing was carried out for all isolated E. coli by using the disc diffusion method. The isolates were phenotypically studied for biofilm-forming ability by cultivation on yeast extract -casamino acids Congo red agar (CRA). Some randomly selected isolates were chosen for the molecular identification of some virulence and resistance genes. Results: A total of 58(48.3%) E. coli isolates could be isolated from the 120 samples. Antimicrobial susceptibility testing exhibited that 91.4%, 79.3%, 79.3%, 74.1%, and 58.6% of the isolates were sensitive to gentamicin, amoxicillin-clavulanic acid, ciprofloxacin, cephalexin, and sulfamethoxazole- trimethoprim, respectively. On the other hand, 91.4% and 70.7% isolates were resistant to cefotaxime and doxycycline, respectively. Cultivation on CRA revealed that 46.6% of isolates were biofilm producers. The molecular detection of resistance and virulence genes declared that all isolates harbored bla TEM , sul 1, tet A, qnr S, bla CTX-M , and fim H with a percentage of 100%, pap C (40%), and hly A (10%). Fim H was the most prevalent biofilm-associated gene. Conclusion: The present study highlights the high prevalence of multi-drug- resistant E. coli associated with bovine endometritis. The detection of the fim H gene is circumstantial evidenced that this gene has a crucial role in biofilm formation in intrauterine pathogenic E. coli .

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Section: Discussionmentioning

confidence: 89%

Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles

Raheel¹,

Hassan²,

Salem³

et al. 2020

J Adv Vet Anim Res

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“…One pure culture of T. pyogenes partially matched the NGS results since the number of reads was lower (11.28%) than those of the most abundant genus, Fusobacterium (74.04%). T. pyogenes cooperates with environmental anaerobes, such as Fusobacterium necrophorum , to produce infections, such as endometritis in cows, and increases the possibility of uterine inflammatory conditions while intensifying disease symptoms [ 54 – 56 ]. Fusobacterium is known to cause sporadic bovine abortion [ 57 , 58 ], but the impact of mixed infections on abortions has not yet been determined.…”

Section: Discussionmentioning

confidence: 99%

Amplicon sequencing of bacterial microbiota in abortion material from cattle

Vidal

Kegler

Posthaus

et al. 2017

Vet Res

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Abortions in cattle have a significant economic impact on animal husbandry and require prompt diagnosis for surveillance of epizootic infectious agents. Since most abortions are not epizootic but sporadic with often undetected etiologies, this study examined the bacterial community present in the placenta (PL, n = 32) and fetal abomasal content (AC, n = 49) in 64 cases of bovine abortion by next generation sequencing (NGS) of the 16S rRNA gene. The PL and AC from three fetuses of dams that died from non-infectious reasons were included as controls. All samples were analyzed by bacterial culture, and 17 were examined by histopathology. We observed 922 OTUs overall and 267 taxa at the genus level. No detectable bacterial DNA was present in the control samples. The microbial profiles of the PL and AC differed significantly, both in their composition (PERMANOVA), species richness and Chao-1 (Mann–Whitney test). In both organs, Pseudomonas was the most abundant genus. The combination of NGS and culture identified opportunistic pathogens of interest in placentas with lesions, such as Vibrio metschnikovii, Streptococcus uberis, Lactococcus lactis and Escherichia coli. In placentas with lesions where culturing was unsuccessful, Pseudomonas and unidentified Aeromonadaceae were identified by NGS displaying high number of reads. Three cases with multiple possible etiologies and placentas presenting lesions were detected by NGS. Amplicon sequencing has the potential to uncover unknown etiological agents. These new insights on cattle abortion extend our focus to previously understudied opportunistic abortive bacteria.

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“…Escherichia coli and other Gram-negative bacteria are the major pathogens associated with endometritis (Janowski et al, 2013;Sens and Heuwieser, 2013;Brodzki et al, 2014;Wagener et al, 2014). Lipopolysaccharides (LPSs) are the major structural components of the cell wall of Gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

Punicalagin protects bovine endometrial epithelial cells against lipopolysaccharide-induced inflammatory injury

Lyu

Chen

Wang

et al. 2017

J. Zhejiang Univ. Sci. B

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Abstract:Objective: Bovine endometritis is one of the most common reproductive disorders in cattle. The aim of this study was to investigate the anti-inflammation potential of punicalagin in lipopolysaccharide (LPS)-induced bovine endometrial epithelial cells (bEECs) and to uncover the underlying mechanisms. Methods: bEECs were stimulated with different concentrations (1, 10, 30, 50, and 100 μg/ml) of LPS for 3, 6, 9, 12, and 18 h. MTT assay was used to assess cell viability and to identify the conditions for inflammatory injury and effective concentrations of punicalagin. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to assess gene expression of pro-inflammatory cytokines. Western blotting was used to assess levels of inflammation-related proteins. Results: Treatment of bEECs with 30 µg/ml LPS for 12 h induced cell injury and reduced cell viability. Punicalagin (5, 10, or 20 µg/ml) pretreatment significantly decreased LPS-induced productions of interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor-α (TNF-α) in bEECs. Molecular research showed that punicalagin inhibited the activation of the upstream mediator nuclear factor-κB (NF-κB) by suppressing the production of inhibitor κBα (IκBα) and phosphorylation of p65. Results also indicated that punicalagin can suppress the phosphorylation of mitogen-activated protein kinases (MAPKs) including p38, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK). Conclusions: Punicalagin may attenuate LPS-induced inflammatory injury and provide a potential option for the treatment of dairy cows with Escherichia coli endometritis.

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Trueperella pyogenes and Escherichia coli as an etiological factor of endometritis in cows and the susceptibility of these bacteria to selected antibiotics

Cited by 24 publications

References 34 publications

Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles

Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles

Amplicon sequencing of bacterial microbiota in abortion material from cattle

Punicalagin protects bovine endometrial epithelial cells against lipopolysaccharide-induced inflammatory injury

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