Truncation of C-terminal amino acids of GH26 endo-mannanase (ManB-1601) affects biochemical properties and stability against anionic surfactants

Monica, P; Mutturi, Sarma; Kapoor, M.

doi:10.1016/j.enzmictec.2022.110031

Enzyme and Microbial Technology

2022

DOI: 10.1016/j.enzmictec.2022.110031

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Truncation of C-terminal amino acids of GH26 endo-mannanase (ManB-1601) affects biochemical properties and stability against anionic surfactants

P Monica

Sarma Mutturi

M. Kapoor

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2024

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“…high a nity for carbohydrate structures, hence enhancing the proximity of the enzyme and its substrate, thereby promoting the substrate degradation (17)(18)(19). The fusion of CBM1 at the C-terminus of the threedomain laccase from Pycnoporus cinnabarinus expressed in A. niger was less thermostable than the native laccase but improved its e ciency for softwood kraft pulp biobleaching (20).…”

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confidence: 99%

Streptomyces small laccase expressed in Aspergillus niger as a new addition for the lignocellulose bioconversion toolbox

Sidar,

Voshol,

El-Masoudi

et al. 2024

Preprint

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Laccases are multi-copper oxidases that are usually composed of three Cu-oxidase domains. Domain one and three house the copper binding sites, and the second domain is involved in forming a substrate-binding cleft. However, Streptomyces species are found to have small laccases (SLAC) that lack one of the three Cu-oxidase domains. This type of SLAC with interesting bioconversion activities have not been reported in Aspergillus niger. In our research, we explored the expression and engineering of the SLAC from Streptomyces leeuwenhoekii C34 in A. niger. Genes encoding two versions of the SLAC were expressed. One encoding the SLAC in its native form and a second encoding the SLAC fused to two N-terminal CBM1 domains. The latter is a configuration also known for specific yeast laccases. Both SLAC variants were functionally expressed in A. niger as shown by in vitro activity assays and proteome analysis. Laccase activity was also analyzed toward bioconversion of lignocellulosic rice straw. From this analysis it was clear that the SLAC activity improved the efficiency of saccharification of lignocellulosic biomass by cellulase enzyme cocktails.

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mentioning

confidence: 99%

Streptomyces small laccase expressed in Aspergillus niger as a new addition for the lignocellulose bioconversion toolbox

Sidar,

Voshol,

El-Masoudi

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Truncation of C-terminal amino acids of GH26 endo-mannanase (ManB-1601) affects biochemical properties and stability against anionic surfactants

Cited by 1 publication

References 64 publications

Streptomyces small laccase expressed in Aspergillus niger as a new addition for the lignocellulose bioconversion toolbox

Streptomyces small laccase expressed in Aspergillus niger as a new addition for the lignocellulose bioconversion toolbox

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