With the challenges that negatively impact tree-based agriculture, landscapes and forests, such as climate change, plant pathogen and insect range expansion, invasive species and limited new pesticides, it is important to introduce new and effective tree protection options. In the last 20 years, pathogens that invade wood i.e. vascular tissues of trees causing wilt, yellowing, premature defoliation, cankers and tree death, have been on the rise. Diplodia corticola causes Bot canker of oak species which can kill trees diminishing the valuable ecological services they provide and reducing profits from wood and cork production. Since this and similar pathogens have difficult biologies because they reside in wood and cause severe internal damage and tree death, their management is difficult or inefficient with classical pesticide application methods that cannot reach and distribute the active ingredient in vascular wood tissues. As practical management options for this and other vascular tissue pathogens of trees are limited, we evaluated efficacy of several trunk injected fungicides in control of D. corticola and compared it with the efficacy of trunk injection of similar compounds for control of Venturia inaequalis and Erwinia amylovora, as two well-studied apple tree pathogens with different or partially similar lifestyles to D. corticola, respectively.