Trypanosoma cruzi epimastigote endocytic pathway: cargo enters the cytostome and passes through an early endosomal network before storage in reservosomes

Porto-Carreiro, Isabel; Attias, Márcia; Miranda, Kildare; Souza, Wanderley de; Cunha-e-Silva, Narcisa L.

doi:10.1078/0171-9335-00112

Cited by 117 publications

(100 citation statements)

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“…13). Quantitative analysis of the ingestion of gold-labeled macromolecules has shown that in epimastigote forms of T. cruzi, about 85% of gold particles associate with the cytostome (Porto-Carrero et al 2000). Epimastigotes that are incubated with acridine orange, a weak base that concentrates in acidic compartments, accumulate dye in the cytopharynx, suggesting that this compartment has an acidic character.…”

Section: The Endocytic Pathwaymentioning

confidence: 99%

Structural organization of Trypanosoma cruzi

Souza

2009

Mem. Inst. Oswaldo Cruz

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Section: The Endocytic Pathwaymentioning

confidence: 99%

Structural organization of Trypanosoma cruzi

Souza

2009

Mem. Inst. Oswaldo Cruz

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“…In epimastigotes, the proliferative form found in the insect vector, the cytostomecytopharynx is the major site for endocytosis (Porto-Carreiro et al, 2000), rather than the 'flagellar pocket', which represents the sole site for endocytosis and exocytosis in other human pathogens from the same family (the Trypanosomatidae), such as Trypanosoma brucei and Leishmania sp. (Webster and Russell, 1993).…”

Section: Introductionmentioning

confidence: 99%

The cytostome-cytopharynx complex ofTrypanosoma cruziepimastigotes disassembles during cell division

Alcantara

Vidal

Souza

et al. 2016

Journal of Cell Science

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The cytostome-cytopharynx complex is the main site for endocytosis in epimastigotes of Trypanosoma cruzi. It consists of an opening at the plasma membrane surface -the cytostome -followed by a membrane invagination -the cytopharynx. In G1/S cells, this structure is associated with two specific sets of microtubules, a quartet and a triplet. Here, we used electron microscopy and electron tomography to build 3D models of the complex at different stages of the cell cycle. The cytostome-cytopharynx is absent in late G2 and M phase cells, whereas early G2 cells have either a short cytopharynx or no visible complex, with numerous vesicles aligned to the cytostome-cytopharynx microtubules. The microtubule quartet remains visible throughout cell division (albeit in a shorter form), and is duplicated during G2/M. In contrast, the microtubule triplet is absent during late G2/M. Cells in cytokinesis have an invagination of the flagellar pocket membrane likely to represent early stages in cytostome-cytopharynx assembly. Cells in late cytokinesis have two fully developed cytostome-cytopharynx complexes. Our data suggest that the microtubule quartet serves as a guide for new cytostome-cytopharynx assembly.

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“…Following binding to the cytostome and flagellar pocket, macromolecules are rapidly internalized in small uncoated vesicles which bud from these structures. The characterization of endocytic compartments in T. cruzi is still controversial (Porto-Carreiro et al, 2000;Figueiredo & Soares, 2000). However, there is a consensus that the endocytic pathway seems to converge to large lysosomerelated organelles called reservosomes.…”

Section: Trypanosomatidesmentioning

confidence: 99%