Trypanosoma cruzi: parasite shed vesicles increase heart parasitism and generate an intense inflammatory response

Torrecilhas, Ana Claúdia; Tonelli, Renata Rosito; Pavanelli, Wander Rogério; Silva, João; Schumacher, Robert I.; Souza, Wanderley de; Silva, Narcisa Cunha e; Abrahamsohn, Ises de Almeida; Colli, Walter; Alves, Maria Júlia M.

doi:10.1016/j.micinf.2008.10.003

Cited by 185 publications

(232 citation statements)

References 30 publications

Supporting

Mentioning

223

Contrasting

Unclassified

Order By: Relevance

“…The pre-treatment of BALB/c mice with vesicles, followed by parasite challenge, could significantly exacerbate parasite load and inflammation of the heart, and it could hasten animal mortality. 38 Here, similarly to the vesicles, purified tGPImucins were also able to modulate trypomastigote invasion during interaction with LLC-MK2 cells. Higher invasion ability was observed for Y strain after the cells were incubated with tGPI-mucins from BZR-Y population and CL strain.…”

Section: Discussionmentioning

confidence: 72%

“…T. cruzi trypomastigotes are able to release extracellular vesicles containing Tc85, TS, and tGPI-mucins that increase parasite invasion and cardiac tissue lesions in mice. 38 To evaluate the ability of purified tGPI-mucins in modulating invasion of LLC-MK2 cells by trypomastigotes, the cells were preincubated with the glycoconjugates (1 μg/mL) from each strain/ populations for 24 hours. Trypomastigotes were able to invade and multiply inside LLC-MK2 cells ( Figure 4A).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Intraspecies Variation in Trypanosoma cruzi GPI-Mucins: Biological Activities and Differential Expression of α-Galactosyl Residues

Soares¹,

Torrecilhas²,

Assis³

et al. 2012

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Intraspecies Variation in Trypanosoma cruzi GPI-Mucins: Biological Activities and Differential Expression of α-Galactosyl Residues

Soares¹,

Torrecilhas²,

Assis³

et al. 2012

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…After loading the filtered parasite supernatant (1 mL), the column was eluted with the same buffer at a flow rate of 0.2 mL/min. Fractions of 1 mL were collected and then screened by nanoparticle tracking analysis (NTA) and enzyme-linked immunosorbent assay (ELISA) to locate the fractions containing the EVs, as previously described [21]. …”

Section: Methodsmentioning

confidence: 99%

“…The samples were coated with a gold layer using a sputtering method (‘sputtering’, © Leica EM 500 SCD, Germany). Then, samples were observed in a Field Emission FEI Quanta 250 FEG scanning electron microscope [10,21]. …”

Section: Methodsmentioning

confidence: 99%

Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells

Ribeiro

Vasconcellos

Soares

et al. 2018

J of Extracellular Vesicle

View full text Add to dashboard Cite

Trypanosoma cruzi, the aetiologic agent of Chagas disease, releases vesicles containing a wide range of surface molecules known to affect the host immunological responses and the cellular infectivity. Here, we compared the secretome of two distinct strains (Y and YuYu) of T. cruzi, which were previously shown to differentially modulate host innate and acquired immune responses. Tissue culture-derived trypomastigotes of both strains secreted extracellular vesicles (EVs), as demonstrated by electron scanning microscopy. EVs were purified by exclusion chromatography or ultracentrifugation and quantitated using nanoparticle tracking analysis. Trypomastigotes from YuYu strain released higher number of EVs than those from Y strain, enriched with virulence factors trans-sialidase (TS) and cruzipain. Proteomic analysis confirmed the increased abundance of proteins coded by the TS gene family, mucin-like glycoproteins, and some typical exosomal proteins in the YuYu strain, which also showed considerable differences between purified EVs and vesicle-free fraction as compared to the Y strain. To evaluate whether such differences were related to parasite infectivity, J774 macrophages and LLC-MK2 kidney cells were preincubated with purified EVs from both strains and then infected with Y strain trypomastigotes. EVs released by YuYu strain caused a lower infection but higher intracellular proliferation in J774 macrophages than EVs from Y strain. In contrast, YuYu strain-derived EVs caused higher infection of LLC-MK2 cells than Y strain-derived EVs. In conclusion, quantitative and qualitative differences in EVs and secreted proteins from different T. cruzi strains may correlate with infectivity/virulence during the host–parasite interaction.

show abstract

“…Immunocytochemical analysis showed that Tc85 (a member of the superfamily gp85 trans-sialidase that is anchored to the plasma membrane via GPI-anchors) is localized in these vesicles. Injection of these vesicles into mice before infection with T. cruzi increased parasitaemia and led to exacerbation of heart lesions and inflammation [46]. All mice previously injected with the vesicles and [11,[24][25][26][27].…”

Section: Exosomes In the Protozoan Trypanosoma Cruzimentioning

confidence: 96%

Exosomes in the Pathogenic Protozoan Trypanosoma Cruzi

Souza¹

2017

Int J Pathol Clin Res

View full text Add to dashboard Cite

Significant advances have recently occurred in the identification and characterization of different types of microvesicles released by eukaryotic cells into the extracellular space. Exosomes are one such type of these vesicles they originate from multivesicular bodies and have received much attention. Here, we review the available data on microvesicles secreted by the pathogenic protozoan Trypanosoma cruzi, which is the causative agent of Chagas disease and that still has a high prevalence in Latin America. The available data show that T. cruzi exosomes contain some key proteins and RNA molecules that contain genetic information and may interfere with transcription. The available information suggests that they play some role in the biology of T. cruzi, including its interaction with host cells, as well as on the pathogenesis of Chagas disease where inflammatory processes and host cell dead occurs as a consequence of parasites, factors released by the parasites, and molecules produced by different host cells that are part of the immunological response.

show abstract

Trypanosoma cruzi: parasite shed vesicles increase heart parasitism and generate an intense inflammatory response

Cited by 185 publications

References 30 publications

Intraspecies Variation in Trypanosoma cruzi GPI-Mucins: Biological Activities and Differential Expression of α-Galactosyl Residues

Intraspecies Variation in Trypanosoma cruzi GPI-Mucins: Biological Activities and Differential Expression of α-Galactosyl Residues

Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells

Exosomes in the Pathogenic Protozoan Trypanosoma Cruzi

Contact Info

Product

Resources

About