Trypanosome infections of the tsetse fly Glossina pallidipes in the Luangwa Valley, Zambia

Woolhouse, Mark; Bealby, K. A.; McNamara, J.J.; Silutongwe, J.

doi:10.1016/0020-7519(94)90164-3

Cited by 36 publications

(29 citation statements)

References 14 publications

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“…This study identified T. vivax as the most common trypanosome species infecting tsetse flies in Western Zambia, occurring as either single or mixed infections. This finding corresponds with that of Woolhouse et al (1994) but contradicts others which record T. congolense as the most common fly trypanosome (Kubi et al, 2007). This discrepancy may be due to two reasons (a) the previous studies report on G. morsitans morsitans rather than G. morsitans centralis and (b) previous studies utilise morphological identification, while in the current study PCR was used, which may identify immature as well as mature infections.…”

Section: Wolbachia Sodalis Sghv and Trypanosome Infectioncontrasting

confidence: 82%

Microbiome frequency and their association with trypanosome infection in male Glossina morsitans centralis of Western Zambia

Mbewe¹,

Mweempwa²,

Guya

et al. 2015

Veterinary Parasitology

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Section: Wolbachia Sodalis Sghv and Trypanosome Infectioncontrasting

confidence: 82%

Microbiome frequency and their association with trypanosome infection in male Glossina morsitans centralis of Western Zambia

Mbewe¹,

Mweempwa²,

Guya

et al. 2015

Veterinary Parasitology

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“…Although three major trypanosome species, Trypanosoma congolense, T. vivax, and T. brucei subspecies, have been reported from cattle and tsetse flies in Zambia (Awan and Sawchuck 1976;Chitambo and Arakawa 1991;Woolhouse et al 1994), their distribution is not clearly elucidated because of limitations in detection and identification techniques. Meanwhile, the most important mode of transmission is mainly cyclic through tsetse flies; to a lesser extent, mechanical transmission may also play a role where hemophagous insects exist.…”

Section: Introductionmentioning

confidence: 99%

Detection of Trypanosoma congolense and T. brucei subspecies in cattle in Zambia by polymerase chain reaction from blood collected on a filter paper

Katakura

Lubinga

Chitambo

et al. 1997

Parasitology Research

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To facilitate epidemiology studies of African trypanosomiasis in cattle in Zambia, we adapted a polymerase chain reaction (PCR) method using blood spotted on filter papers. For easy preparation of template DNA from the dried blood, we adapted a simple DNA extraction method using Chelex-100, an anion-exchange resin. Using primers directed for repetitive nuclear DNA sequences, species-specific DNA amplifications were detected from the blood of rats infected with Zambian isolates of T. congolense and T. brucei subspecies. The method was sensitive enough to detect a single trypanosome for both species. In the Eastern Province of Zambia, 240 cattle were examined for motile flagellates in the buffy coat by the microhematocrit method, and 100 of them were positive for the test. These 100 animals were further examined by thin blood smears and PCR for species identification. The thin blood smear revealed 62 and 14 animals with T. congolense and T. brucei subspecies infection, respectively, whereas the PCR detected 73 of the former and 38 of the latter species. These results indicate that dried blood spots on filter papers are a useful source of DNA for detection of African trypanosomes by PCR.

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“…There are specific foci for these flies. The flies that are good vectors for T. b. rhodesiense (namely, Glossina morsitans morsitans, Glossina swynnertinii, Glossina auteni, Glossina longipenis, Glossina pallidipes, Glossina bravepalpis, and Glossina fuscipes fuscipes [5][6][7] ) are mainly confined to the southwestern part of Africa, while the flies that are good vectors for T. b. gambiense (namely, Glossina fuscipes fuscipes, Glossina tachinoides, and Glossina morsistans [8][9][10] ) are mainly confined to the western part of the continent. However, there is an overlap of these flies, mainly in…”

Section: Introductionmentioning

confidence: 99%

Polymerase chain reaction identification of <i>Trypanosoma brucei rhodesiense</i> in wild tsetse flies from Nkhotakota Wildlife Reserve, Malawi

et al. 2017

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BackgroundTrypanosoma brucei rhodesiense is the causative agent of acute human African trypanosomiasis. Identification of T. b. rhodesiense in tsetse populations is essential for understanding transmission dynamics, assessng human disease risk, and monitoring spatiotemporal trends and impact of control interventions. Accurate detection and characterisation of trypanosomes in vectors relies on molecular techniques. For the first time in Malawi, a molecular technique has been used to detect trypanosomes in tsetse flies in Nkhotakota Wildlife Reserve. MethodsA polymerase chain reaction (PCR) technique was used to identify the serum resistance associated (SRA) gene of T. b. rhodesiense in tsetse flies. Of 257 tsetse flies that were randomly caught, 42 flies were dissected for microscopic examination. The midguts of 206 flies were positive and were individually put in eppendorf tubes containing phosphate-buffered saline (PBS buffer) for DNA extraction. Internal transcribed spacer (ITS)-PCR was first used to isolate all trypanosome species from the flies. TBR PCR was then used to isolate the Trypanozoon group. T. brucei-positive samples were further evaluated by SRA PCR for the presence of the SRA gene.

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Trypanosome infections of the tsetse fly Glossina pallidipes in the Luangwa Valley, Zambia

Cited by 36 publications

References 14 publications

Microbiome frequency and their association with trypanosome infection in male Glossina morsitans centralis of Western Zambia

Microbiome frequency and their association with trypanosome infection in male Glossina morsitans centralis of Western Zambia

Detection of Trypanosoma congolense and T. brucei subspecies in cattle in Zambia by polymerase chain reaction from blood collected on a filter paper

Polymerase chain reaction identification of <i>Trypanosoma brucei rhodesiense</i> in wild tsetse flies from Nkhotakota Wildlife Reserve, Malawi

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