Trypsin Inhibitory Activity of Water Extracts from Ecklonia cava as Affected by Temperature and pH

정슬아,; 김꽃봉우리,; 김민지,; Kim, Dong Hyun; 선우찬,; 김현지,; 정다현,; 정희예,; 김태완,; 조영제,; 안동현,

doi:10.3746/jkfn.2012.41.6.840

Cited by 4 publications

(1 citation statement)

References 32 publications

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“…Moisture content was carried out with ambient pressure drying, and crude ash was measured by dry ashing method. Carbohydrate was calculated based on 100% minus the sum of crude lipid, crude protein, moisture, and ash contents (Jung et al, 2012).…”

Section: Proximate Analysismentioning

confidence: 99%

Characteristics and anti-inflammatory effects of the enzymatically extracted polysaccharides of Sargassum fulvellum using crude enzyme from Shewanella oneidensis PKA 1008

Xu¹,

Jeong²,

Lee³

et al. 2022

IFRJ

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Alginic acid is a polysaccharide obtained from brown algae, and its oligosaccharide has various functions such as antiviral, antitumor, immunoregulation, and antioxidant. However, because of its high viscosity, numerous studies have degraded the alginic acid by enzymes to improve its utilisation. In the present work, we characterised Sargassum fulvellum enzymatic extract (SFEE) using polysaccharide-degrading enzyme obtained from Shewanella oneidensis PKA 1008, and investigated its anti-inflammatory potential. S. fulvellum powder and crude enzyme were mixed at a ratio of 1:1 (v/v), and reacted at 30°C for 0 - 48 h to obtain the optimum degrading time. The changes in pH, colour, reducing sugar, and viscosity of SFEE were determined. The anti-inflammatory activity of SFEE was confirmed by measuring the expression level of nitric oxide (NO) and pro-inflammatory cytokines (IL-6, TNF-α, and L-1β) in RAW 264.7 macrophage cell line. The reducing sugar content was found to increase 2.75-fold at 24 h as compared to that at the initial reaction point, but pH and viscosity decreased significantly with increasing reaction time. SFEE showed a high inhibitory effect on the levels of NO and pro-inflammatory cytokines. SFEE thus has great potential for development as a functional food and therapeutic material owing to its anti-inflammatory effect.

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Section: Proximate Analysismentioning

confidence: 99%

Characteristics and anti-inflammatory effects of the enzymatically extracted polysaccharides of Sargassum fulvellum using crude enzyme from Shewanella oneidensis PKA 1008

Xu¹,

Jeong²,

Lee³

et al. 2022

IFRJ

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Antioxidant and Antiobesity Activity of Natural Color Resources

황초롱¹,

탁현민²,

강민정³

et al. 2014

Journal of Life Science

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Chrysanthemum, Sandalwood Red, Himalayan Rhubarb, and Madder. Total phenol content was the highest in Catechu extract, at 348.25 mg/g. DPPH, ABTS radical scavenging activity and ferric reducing antioxidant power (FRAP) were also higher in Catechu extract. Bleaching inhibition activity in the β-carotene linoleic acid system was the highest in Black locust extract, as was α-Glucosidase inhibition activity. α-Amylase inhibition activity was the highest in Catechu extract. Trypsin inhibition activity of Black locust extract was greater than 60%, and αchymotrypsin inhibition activity of Catechu extract was greater than 40%. Lipase inhibition activity was the highest Black locust extract, at 52.73%. Viability of 3T3-L1 cells was not affected by treatment with extracts at concentrations of 1.25~25 μg/ml. Lipid accumulation in the 3T3-L1 cells was the lowest following treatment with Catechu extract, at 55.8%, and this extract also inhibited adipocyte differentiation. These results suggest that the Catechu and Black locust extracts have high antioxidant and antiobesity activities and can be useful ingredients in functional foods.

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Isolation of Eckol from Ecklonia cava via Centrifugal Partition Chromatography (CPC) and Characterization of it's Anti-inflammatory Activity

김윤택¹,

이지혁²,

고주영³

et al. 2015

Korean Journal of Fisheries and Aquatic Sciences

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Phlorotannins and marine algal polyphenols, including dieckol, 6,6-bieckol, phloroglucinol, phlorofucofuroeckol-A, and eckol, were isolated from brown seaweeds. These compounds have beneficial bioactivities, and Ecklonia cava has become widely used for the extraction and isolation of phlorotannins. Eckol, in particular, has been to shown to have antioxidant, anti-inflammatory, anticoagulatory, and photoprotective properties. However, due to its low abundance in weaweed, the isolation and purification of eckol are difficult. Its limited availability renders the isolation and purification of eckol labor-intensive processes. Centrifugal partition chromatography (CPC) is an efficient technique for the isolation and purification of eckol. In this study, eckol was isolated from the ethyl acetate fraction of the 70% ethanol extract of E. cava using CPC with a two-phase solvent system of a n-hexane:EtOAc:methanol:water (2:8:3:7, v/v) solution. The purity and anti-inflammatory activity of the isolated eckol were verified by high-performance liquid chromatography and by assaying lipopolysaccharide-induced inflammatory responses in an immortalized murine BV2 microglial cell line, respectively. In conclusion, CPC is a useful technique for simple and efficient isolation of eckol from E. cava.

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Trypsin Inhibitory Activity of Water Extracts from Ecklonia cava as Affected by Temperature and pH

Cited by 4 publications

References 32 publications

Characteristics and anti-inflammatory effects of the enzymatically extracted polysaccharides of Sargassum fulvellum using crude enzyme from Shewanella oneidensis PKA 1008

Characteristics and anti-inflammatory effects of the enzymatically extracted polysaccharides of Sargassum fulvellum using crude enzyme from Shewanella oneidensis PKA 1008

Antioxidant and Antiobesity Activity of Natural Color Resources

Isolation of Eckol from Ecklonia cava via Centrifugal Partition Chromatography (CPC) and Characterization of it's Anti-inflammatory Activity

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