Tryptophan-Assisted Synthesis Reduces Bimetallic Gold/Silver Nanoparticle Cytotoxicity and Improves Biological Activity

Abstract

Aiming to reduce the potential in vivo hepato-and nephrotoxicity of Ag/Au bimetallic nanoparticles (NPs) stabilized by sodium dodecyl sulphate (SDS), an approach involving a simultaneous reduction of silver nitrate and tetrachlorauratic acid using tryptophan (Trp) as a reducing/stabilizing agent was applied during NP synthesis. The obtained Ag/Au/Trp NPs (5–15 nm sized) were able to form stable aggregates with an average size of 370–450 nm and were potentially less toxic than Ag/Au/SDS in relation to a mouse model system based on clinical biochemical parameters and oxidative damage product estimation. Ag/Au/Trp NPs were shown to exhibit anticancer activity in relation to a Lewis lung carcinoma model. The data generated from the present study support the fact that the use of tryptophan in NP synthesis is effective in attenuating the potential hepatotoxicity and nephrotoxicity of NPs during their in vivo application.

Plasmonic Mono- And Bimetallic Nanoparticles of Gold and Silver as Promising Tools of Complex Therapy of Cancer

“…Так, було досягнуто зменшення розміру та маси первинної пухлини, середнього розміру метастазів і підвищення рівня виживання загалом. Використання триптофану сприяло зниженню гепато-і нефротоксичного впливу нанокомпозиту [4]. Результати досліджень біологічного впливу наночастинок, одержаних у присутності триптофану, спонукали до вдосконалення експериментальних методик їх синтезу з метою підвищення стійкості колоїдних розчинів та визначення оптимального складу частинок для досягнення найвищої біологічної ефективності.…”

Synthesis of pH sensitive gold nanoparticles for potential application in radiosensitization

“…Iosin et al has synthesized anisotropic GNPs suitable for SERS application at 80°C by reduction of HAuCl 4 by Trp in aqueous medium [31]. Recently, Trp reduced/stabilized bimetallic Au-Ag was synthesized by Shmarakov et al for effective attenuation in potential hepatotoxicity and nephrotoxicity during their in vivo application [36]. The particles synthesized were found to be polydispersed in size.…”

Bimetallic gold/silver alloy nanoparticles prepared in the presence of tryptophan

“…Their bimetallic composition could act more effectively in cancer treatment due to synergetic effect. Also, the toxicity of bimetallic NPs can be greatly decreased through the use of essential amino acid as reducing and stabilizing agent [5]. Here we used tryptophan for reduction of metal ions and also for stabilization of NPs in the colloid.…”