TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the <i>MIR454</i>-FAM83A-TSPAN1 axis in pancreatic cancer

Zhou, Cefan; Liang, Yanyan; Zhou, Li; Yan, Yanan; Liu, Nanxi; Zhang, Rui; Huang, Yuan; Wang, Ming; Tang, Yongfei; Ali, Declan W.; Wang, Yefu; Michalak, Marek; Chen, Xing-Zhen; Tang, Jingfeng

doi:10.1080/15548627.2020.1826689

Cited by 69 publications

(61 citation statements)

References 71 publications

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“…TSPAN1 (Tetraspanin 1) is a member of the Tetraspanins family of proteins whose important feature is their ability to aggregate with one another or various other transmembrane receptors, to become TSPAN-enriched microdomains (TEMs), which are essential in determining the fundamental biological activities such as cell adhesion, proliferation, and cell motility [12,14]. A few studies have reported that TSPAN1 could play a significant role in the progression of PC [15]. A study by Hou et al showed that transfection with siRNA-targeting TSPAN1 significantly decreased proliferation, increased apoptosis, and reduced migration and invasion of AsPC-1 and PANC-1 cells, which suggested that TSPAN1 was involved in the PC of migration and invasion [16].…”

Section: Discussionmentioning

confidence: 99%

“…A few studies have reported that TSPAN1 could play a significant role in the progression of PC [ 15 ]. A study by Hou et al showed that transfection with siRNA-targeting TSPAN1 significantly decreased proliferation, increased apoptosis, and reduced migration and invasion of AsPC-1 and PANC-1 cells, which suggested that TSPAN1 was involved in the PC of migration and invasion [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

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Screening and Validation of Independent Predictors of Poor Survival in Pancreatic Cancer

Shui

Cai

Changyong

et al. 2021

Pathol. Oncol. Res.

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Pancreatic cancer is a digestive system malignant tumor with high mortality and poor prognosis, but the mechanisms of progression remain unclear in pancreatic cancer. It’s necessary to identify the hub genes in pancreatic cancer and explore the novel potential predictors in the prognosis of pancreatic cancer. We downloaded two mRNA expression profiles from Gene Expression Omnibus and The Cancer Genome Atlas Pancreatic Cancer (TCGA-PAAD) datasets to screen the commonly differentially expressed genes in pancreatic cancer by limma package in R. Subsequently, measurement of the functional similarity among the 38 DEGs in common was performed to identify the hub genes using GOSemSim package. Then, survival analysis and Cox regression were applied to explore prognosis-related hub genes using the survival package. Statistics analysis by two-tailed Student’s t-test or one-way based on TCGA-PAAD datasets and qPCR detection in clinical samples were performed to explore the correlations between expression of hub genes in pancreatic cancer tissues and clinical parameters. Based on integrated analysis of TCGA and GEO datasets, we screened 38 DEGs in common, which were all up-regulated. The functional similarity results showed that 10 DEGs including TSPAN1, MSLN, C1orf116, PKP3, CEACAM6, BAIAP2L1, PPL, RAB25, ERBB3, and AP1M2 in the DEGs in common, which had the higher average functional similarity, were considered as the hub genes. Survival analysis results and Cox regression analysis showed that TSPAN1, CEACAM6, as well as ERBB3 were all associated with poor overall survival of PC. qPCR results showed that the expression levels of TSPAN1 and ERBB3 were significantly upregulated in the PC tissues. The statistical analysis results revealed that TSPAN1 expression correlated significantly with histologic grade, T stage, clinical stage, and vital status by two-tailed Student’s t-test or one-way ANOVA; ERBB3 expression correlated significantly with T stage, clinical stage, and vital status by two-tailed Student’s t-test or one-way ANOVA. We found that TSPAN1 and ERBB3 could be independent predictors of poor survival in pancreatic cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Screening and Validation of Independent Predictors of Poor Survival in Pancreatic Cancer

Shui

Cai

Changyong

et al. 2021

Pathol. Oncol. Res.

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“…Thus, the study suggests that manipulation of this Notch-dependent circuit has therapeutic potential in the treatment of PDAC metastases. This study provided potential therapeutic targets and solid preclinical evidence for PDAC treatment by interrupting the feedback between cancer cells and macrophages with targeted inhibitors [88].…”

Section: Circulating Mirnasmentioning

confidence: 98%

The Role of microRNA in Pancreatic Cancer

et al. 2021

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MicroRNAs (miRNAs) are small ribonucleic acid molecules that play a key role in regulating gene expression. The increasing number of studies undertaken on the functioning of microRNAs in the tumor formation clearly indicates their important potential in oncological therapy. Pancreatic cancer is one of the deadliest cancers. The expression of miRNAs released into the bloodstream appears to be a good indicator of progression and evaluation of the aggressiveness of pancreatic cancer, as indicated by studies. The work reviewed the latest literature on the importance of miRNAs for pancreatic cancer development.

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“…The intensity of staining was divided into four grades (intensity scores): no staining (0), light brown (1), brown (2), and dark brown (3). The overall staining score was determined by the formula: overall scores = percentage score × intensity score [25]. The sources and dilution of the primary antibody were shown in the table2.…”

Section: Immunohistochemistry Assaymentioning

confidence: 99%

Interaction between envelope protein of Jaagsiekte Sheep Retrovirus and Hippo signaling pathway is inferred from transcriptome analysis of naturally infected Ovine Pulmonary Adenomatosis

Duan

Yang

Zhang

et al. 2021

Preprint

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Background Ovine pulmonary adenomatosis (OPA) is a contagious lung epithelial tumor of sheep caused by jaagsiekte sheep retrovirus (JSRV), which causes severe economic losses for the sheep industry in the world. The specific oncogenic mechanism of JSRV is not yet clarified. Methods In this study, RNA was extracted from lung tissues of 3 naturally infected OPA cases and 3 healthy individuals for transcriptome sequencing (RNA-Seq). Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to confirm the sequencing data. Immunohistochemistry (IHC) and western blot (WB) were performed to confirm the signaling pathway enriched by DEGs that was activated in naturally infected OPA cases. Cell viability, wound-healing, transwell and colony formation assays were performed to assess the cell malignant transformation of sheep trophoblast cells (STCs) transformed with JSRV- env lentivirus in vitro, and then WB was performed to confirm the signaling pathway that had been validated in the lung tissues. Results A total of 366 DEGs (154 up-regulated and 212 down-regulated) were identified by RNA-Seq of lung tissues of naturally infected OPA cases and healthy individuals. GO analysis showed that 366 DEGs were significantly enriched in 178 GO terms, including 114 biological processes, 19 cellular components and 45 molecular functions. KEGG analysis showed that the DEGs mainly enriched in cell proliferation, differentiation, apoptosis and migration, such as PI3K/Akt/mTOR, MAPK and Hippo signaling pathway, and Hippo signaling pathway has never been reported in naturally infected OPA cases. qRT-PCR results of 10 DEGs which were selected randomly were consistent with RNA-Seq results. The protein expression of Hippo signaling pathway were up-regulated in naturally infected OPA lung tissues. Cell viability, wound-healing, transwell and colony formation assays confirmed that JSRV- env lentivirus caused malignant transformation of STCs and JSRV Env increased the protein expression of Hippo signaling pathway. Conclusions This research first identified the changes in the transcriptome level of naturally infected OPA lung tissues. These data confirm that the Hippo signaling pathway is involved in the mechanism of OPA, clarify the interaction between Hippo signaling pathway and JSRV Env, provide further evidence for the tumorigenic mechanism of JSRV.

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TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer

Cited by 69 publications

References 71 publications

Screening and Validation of Independent Predictors of Poor Survival in Pancreatic Cancer

Screening and Validation of Independent Predictors of Poor Survival in Pancreatic Cancer

The Role of microRNA in Pancreatic Cancer

Interaction between envelope protein of Jaagsiekte Sheep Retrovirus and Hippo signaling pathway is inferred from transcriptome analysis of naturally infected Ovine Pulmonary Adenomatosis

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