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Podgornik, Aleš; Barut, Miloš; Mihelič, Igor; Štrancar, Aleš

doi:10.1016/s0301-4770(03)80020-9

Cited by 9 publications

(9 citation statements)

References 29 publications

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“…Due to attractive monolith features, especially pronounced for very large molecules, it is not surprising that there is plethora of publications describing monolith preparation, characterization, and applications . Being implemented in many different areas, various monolith formats have been described.…”

Section: Introductionmentioning

confidence: 99%

“…Besides in miniaturized formats, monoliths are routinely prepared also on analytical scale e.g. high throughput screening (HTS) in the format of in 96‐well plates or tips , in sheet format of several centimeters size but thickness of only several 10 μm used for planar chromatography , in format of conventional analytical columns or short analytical columns in disk format , as well as in large volume formats intended for purification .…”

Section: Introductionmentioning

confidence: 99%

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Application of monoliths for bioparticle isolation

Podgornik

Krajnc

2012

J of Separation Science

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Monoliths are today probably the most studied chromatographic supports. There are plethora of publications dealing with different aspects of their preparation, characterization, and applications. The reason for this interest is their inherent properties related to their particular structure, like ease of preparation in various volumes, fast analytics at low pressure and room temperature, and high productivity as a consequence of flow-unaffected properties, especially important for isolation of large biological molecules. Because of that, structure of several monoliths was optimized for analytics and purification of biologic nanoparticles like viruses, virus-like particles (VLPs), cells structures, or even intact cells. In this review, some recent applications of monoliths in the field of bioparticle isolation are described and results are discussed in terms of particular monolith properties.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Application of monoliths for bioparticle isolation

Podgornik

Krajnc

2012

J of Separation Science

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“…Once the structure of the resin is equal for columns of different volumes, chromatographic methods can be easily transferred over various process scales preserving resolution [38]. This was confirmed for a mixture of test proteins, but also on real protein samples [39] (see Fig.…”

Section: Introductionmentioning

confidence: 62%

Methacrylate‐based short monolithic columns: Enabling tools for rapid and efficient analyses of biomolecules and nanoparticles

Barut

Podgornik

Urbas

et al. 2008

J of Separation Science

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This review describes the novel chromatography stationary phase--a porous monolithic methacrylate-based polymer--in terms of the design of the columns and some of the features that make these columns attractive for the purification of large biomolecules. We first start with a brief summary of the characteristics of these large molecules (more precisely large proteins like immunoglobulins G and M, plasmid deoxyribonucleic acid (DNA), and viral particles), and a list of some of the problems that were encountered during the development of efficient purification processes. We then briefly describe the structure of the methacrylate-based monolith and emphasize the features which make them more than suitable for dealing with large entities. The highly efficient structure on a small scale can be transferred to a large scale without the need of making column modifications, and the various approaches of how this is accomplished are briefly presented in this paper. This is followed by presenting some of the examples from the bioprocess development schemes, where the implementation of the methacrylate-based monolithic columns has resulted in a very efficient and productive process. Following this, we move back to the analytical scale and demonstrate the efficiency of the monolithic column--where the mass transfer between the stationary and mobile phase is greatly enhanced--for the in-process and final control of the new therapeutics. The combination of an efficient structure and the appropriate hardware results in separations of proteins with residence time less than 0.1 s.

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“…The number of reported applications of large‐volume monolith adsorbents is fairly low despite the successful implementation of small‐scale monolith adsorbents in biomolecules purification . Arrua et al.…”

Section: Bottlenecks In Large‐volume Monolith Preparationmentioning

confidence: 99%

Challenges and strategies in the preparation of large-volume polymer-based monolithic chromatography adsorbents

2014

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To date, the number of published reports on the large-volume preparation of polymer-based monolithic chromatography adsorbents is still lacking and is of great importance. Many critical factors need to be considered when manufacturing a large-volume polymer-based monolith for chromatographic applications. Structural integrity, validity, and repeatability are thought to be the key factors determining the usability of a large-volume monolith in a separation process. In this review, we focus on problems and solutions pertaining to heat dissipation, pore size distribution, "wall channel" effect, and mechanical strength in monolith preparation. A template-based method comprising sacrificial and nonsacrificial techniques is possibly the method of choice due to its precise control over the porous structure. However, additional expensive steps are usually required for the template removal. Other strategies in monolith preparation are also discussed.

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Tubes

Cited by 9 publications

References 29 publications

Application of monoliths for bioparticle isolation

Application of monoliths for bioparticle isolation

Methacrylate‐based short monolithic columns: Enabling tools for rapid and efficient analyses of biomolecules and nanoparticles

Challenges and strategies in the preparation of large-volume polymer-based monolithic chromatography adsorbents

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