Tubular Vascular Endothelial Growth Factor-A, Erythropoietin, and Medullary Vessels

Tufró, Alda

doi:10.1681/asn.2014101004

Cited by 4 publications

(1 citation statement)

References 18 publications

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“…Similar to the histologic findings, the reduction in functional vessels assessed by in vivo mCT was observed before the onset of interstitial fibrosis ( Figure 2B). 7,[22][23][24] We confirmed the continuous reduction of vessel functionality in Alport and UUO mice. For both models, in vivo mCT showed significantly reduced rBV values in diseased versus control kidneys ( Figure 5, A and B for UUO; Figure 5, D and E for Alport mice, respectively).…”

Section: Peritubular Capillary Rarefaction Correlates With Fibrosis Isupporting

confidence: 71%

Quantitative Micro-Computed Tomography Imaging of Vascular Dysfunction in Progressive Kidney Diseases

Ehling¹,

Bábíčková

Gremse

et al. 2016

Journal of the American Society of Nephrology

124

131

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Progressive kidney diseases and renal fibrosis are associated with endothelial injury and capillary rarefaction. However, our understanding of these processes has been hampered by the lack of tools enabling the quantitative and noninvasive monitoring of vessel functionality. Here, we used micro-computed tomography (mCT) for anatomical and functional imaging of vascular alterations in three murine models with distinct mechanisms of progressive kidney injury: ischemia-reperfusion (I/R, days 1-56), unilateral ureteral obstruction (UUO, days 1-10), and Alport mice (6-8 weeks old). Contrast-enhanced in vivo mCT enabled robust, noninvasive, and longitudinal monitoring of vessel functionality and revealed a progressive decline of the renal relative blood volume in all models. This reduction ranged from 220% in early disease stages to 261% in late disease stages and preceded fibrosis. Upon Microfil perfusion, high-resolution ex vivo mCT allowed quantitative analyses of three-dimensional vascular networks in all three models. These analyses revealed significant and previously unrecognized alterations of preglomerular arteries: a reduction in vessel diameter, a prominent reduction in vessel branching, and increased vessel tortuosity. In summary, using mCT methodology, we revealed insights into macro-to-microvascular alterations in progressive renal disease and provide a platform that may serve as the basis to evaluate vascular therapeutics in renal disease.

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Section: Peritubular Capillary Rarefaction Correlates With Fibrosis Isupporting

confidence: 71%