Tubulins in Aspergillus nidulans

Oakley, Berl R

doi:10.1016/j.fgb.2003.11.013

Cited by 60 publications

(41 citation statements)

References 29 publications

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“…Three groups have been identified: two genes encoding the tubulin α-chain and two genes encoding the tubulin β-chain, in addition to a tubulin γ-chain gene, exactly as in S. cerevisiae, A. nidulans and other filamentous fungi ( Oakley, 2004). Likewise, one tubulin α-chain, two tubulin β-chain and one γ-chain genes are present in U. maydis ( Banuett et al, 2008).…”

Section: Genes Involved In the Tubulin Cytoskeletonmentioning

confidence: 99%

Hyphal and cytoskeleton polarization in Tuber melanosporum: A genomic and cellular analysis

Amicucci

Balestrini

Kohler

et al. 2011

Fungal Genetics and Biology

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Filamentous polarized growth involves a series of events including polarization of the cytoskeleton to selected growth sites, and the transport of secretory vesicles containing the components required for growth. The availability of fungal genome sequences has recently led to the identification of a large number of proteins involved in these processes. We have explored the Tuber melanosporum genome sequence by searching for homologs of genes known to play crucial roles in the morphogenesis and cell polarity of yeasts and filamentous fungi. One hundred and forty-nine genes have been identified and functionally grouped according to the deduced amino acid sequences (44 genes involved in cell polarity/morphogenesis, 39 belonging to the actin cytoskeleton and 66 involved in membrane dynamics, septation and exocytosis). A detailed gene annotation has shown that most components of the cell polarity machinery, morphogenesis and cytoskeleton found in yeasts and filamentous fungi are conserved, although the degree of similarity varies from strong to weak. Microscopic analysis of quick-frozen truffle hyphae detected the characteristic subcellular components of the hyphal tip in septate filamentous fungi, while transcript profiles revealed a moderately variable pattern during the biological cycle.

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Section: Genes Involved In the Tubulin Cytoskeletonmentioning

confidence: 99%

Hyphal and cytoskeleton polarization in Tuber melanosporum: A genomic and cellular analysis

Amicucci

Balestrini

Kohler

et al. 2011

Fungal Genetics and Biology

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“…Resistance to MBC fungicides is conferred by single nucleotide mutations in the β-tubulin gene that cause an amino acid substitution preventing binding at the target site (15,22). Resistance has been associated with mutations in the β-tubulin gene in many fungal species including Venturia inaequalis, Monilinia fructicola, Monilinia laxa, Colletotrichum cereale, Cladobotryum dendroides, and others (3,4,15,16,17,20,31,32).…”

Section: Introductionmentioning

confidence: 99%

Resistance of Kansas Sclerotinia homoeocarpa Isolates to Thiophanate-Methyl and Determination of Associated β-Tubulin Mutation

Ostrander

Todd

Kennelly

2014

Plant Health Progress

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Ostrander, J. C., Todd, R. B., and Kennelly, M. M. 2014. Resistance of Kansas Sclerotinia homoeocarpa isolates to thiophanate-methyl and determination of associated β-tubulin mutation. Plant Health Progress doi:10.1094/PHP-RS-13-0120.Eighty-two isolates of Sclerotinia homoeocarpa from 12 sites in Kansas were evaluated for in vitro sensitivity to the methyl benzimidazole carbamate (MBC) fungicide thiophanate-methyl at the discriminatory dose of 10 µg/ml. Seventeen isolates were sensitive to thiophanate-methyl and the remaining isolates were resistant. Of the 65 isolates from golf course putting greens, two isolates were sensitive and the remaining 63 isolates were resistant. Six resistant and five sensitive isolates were also evaluated in greenhouse assays on fungicide-treated plants. The isolates that were sensitive to thiophanate-methyl in vitro did not cause any disease on thiophanate-methyl-treated plants, and those that were resistant in vitro caused blighting on treated plants equivalent to the nontreated controls. The entire β-tubulin gene was sequenced for four resistant and four sensitive isolates. The resistant isolates all harbored a substitution of alanine for glutamic acid at codon 198 (E198A). These results provide a starting point for further surveys and monitoring of fungicide sensitivity.

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“…α-and β-tubulin are related proteins, which form a heterodimer that is the major building block of microtubules. γ-tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly (Oakley, 2004). Besides progress on functions have been made, studies are being initiated to explore other important things, e.g., the mechanisms of regulation of microtubule dynamics in vivo, non-nucleation functions of γ-tubulin, and interactions of tubulins with kinesin, motor proteins and microtubule associated proteins.…”

Section: Introductionmentioning

confidence: 99%