TUG1 Represses Apoptosis, Autophagy, and Inflammatory Response by Regulating miR-27a-3p/SLIT2 in Lipopolysaccharide-Treated Vascular Endothelial Cells

Dong, Yuanyuan; Fan, Gongchun; Li, Yanhong; Zhou, Qin

doi:10.1016/j.jss.2020.05.102

Cited by 17 publications

(14 citation statements)

References 40 publications

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“…LC3 was considered to play regulatory roles in autophagy (Wang et al 2020). In present study, (Zheng et al 2020;Zhou et al 2020) we found that CRY2 knockdown or honokil reversed the effect of miR-27a-3p inhibitor on osteogenic differentiation via regulating apoptosis and autophagy. Furthermore, co-transfection of (Wang et al 2020) miR-27a-3p inhibitor and CRY2 shRNA or honokil induced cell autophagy via mediation of LC3.…”

Section: Discussionsupporting

confidence: 62%

“…Consistent with this view, our data revealed that miR-27a-3p could mediate CRY2/ERK1/2 in osteoblasts and downregulation of miR-27a-3p induced apoptosis in osteoblasts via mediation of CRY2/ERK1/2. Dong Y et al found that miR-27a-3p could regulate the autophagy in LPS-treated HUVECs through targeting SLIT2 (Dong et al 2020;Xie et al 2020). LC3 was considered to play regulatory roles in autophagy (Wang et al 2020).…”

Section: Discussionmentioning

confidence: 99%

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MiR-27a-3p promotes the osteogenic differentiation by activating CRY2/ERK1/2 axis

Ren

Yao

Wang

et al. 2021

Mol Med

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Background Osteoporosis seriously disturbs the life of people. Meanwhile, inhibition or weakening of osteogenic differentiation is one of the important factors in the pathogenesis of osteoporosis. It was reported that miR-27a-3p reduced the symptoms of osteoporosis. However, the mechanism by which miR-27a-3p in osteogenic differentiation remains largely unknown. Methods To induce the osteogenic differentiation in MC3T3-E1 cells, cells were treated with osteogenic induction medium (OIM). RT-qPCR was used to evaluate the mRNA expression of miR-27a-3p and CRY2 in cells. The protein levels of CRY2, Runt-related transcription factor 2 (Runx2), osteopontin (OPN), osteocalcin (OCN) and the phosphorylation level of extracellular regulated protein kinases (ERK) 1/2 in MC3T3-E1 cells were evaluated by western blotting. Meanwhile, calcium nodules and ALP activity were tested by alizarin red staining and ALP kit, respectively. Luciferase reporter gene assay was used to analyze the correlation between CRY2 and miR-27a-3p. Results The expression of miR-27a-3p and the phosphorylation level of ERK1/2 were increased by OIM in MC3T3-E1 cells, while CRY2 expression was decreased. In addition, OIM-induced increase of calcified nodules, ALP content and osteogenesis-related protein expression was significantly reversed by downregulation of miR-27a-3p and overexpression of CRY2. In addition, miR-27a-3p directly targeted CRY2 and negatively regulated CRY2. Meanwhile, the inhibitory effect of miR-27a-3p inhibitor on osteogenic differentiation was reversed by knockdown of CRY2 or using honokiol (ERK1/2 signal activator). Furthermore, miR-27a-3p significantly inhibited the apoptosis of MC3T3-E1 cells treated by OIM. Taken together, miR-27a-3p/CRY2/ERK axis plays an important role in osteoblast differentiation. Conclusions MiR-27a-3p promoted osteoblast differentiation via mediation of CRY2/ERK1/2 axis. Thereby, miR-27a-3p might serve as a new target for the treatment of osteoporosis.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

MiR-27a-3p promotes the osteogenic differentiation by activating CRY2/ERK1/2 axis

Ren

Yao

Wang

et al. 2021

Mol Med

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“…While MALAT1 and NEAT1 promoted sepsis-induced inflammation in organ injury, TUG1 exhibited protective effects by inhibiting apoptosis, promoting cell proliferation, and downregulating immune response in sepsis. TUG1 was found to sponge miR-27a-3p, and enhance slit guidance ligand 2 (SLIT2) expression to inhibit apoptosis and autophagy of vascular endothelial cells (55). This finding is consistent with that of Wang et al who observed the overexpression of TUG1 could decrease TNF-a expression level and reduce sepsis-induced apoptosis of cardiomyocytes via ing miR-27a, which had a protective effect against sepsis-induced myocardial injury (56).…”

Section: The Roles Of Lncrnas In Sepsissupporting

confidence: 81%

Section: The Roles Of Lncrnas In Sepsismentioning

confidence: 99%

Long Non-Coding RNAs as Biomarkers and Therapeutic Targets in Sepsis

et al. 2021

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Sepsis, an infection-induced systemic inflammatory disorder, is often accompanied by multiple organ dysfunction syndromes with high incidence and mortality rates, and those who survive are often left with long-term sequelae, bringing great burden to social economy. Therefore, novel approaches to solve this puzzle are urgently needed. Previous studies revealed that long non-coding RNAs (lncRNAs) have exerted significant influences on the process of sepsis. The aim of this review is to summarize our understanding of lncRNAs as potential sepsis-related diagnostic markers and therapeutic targets, and provide new insights into the diagnosis and treatment for sepsis. In this study, we also introduced the current diagnostic markers of sepsis and discussed their limitations, while review the research advances in lncRNAs as promising biomarkers for diagnosis and prognosis of sepsis. Furthermore, the roles of lncRNAs in sepsis-induced organ dysfunction were illustrated in terms of different organ systems. Nevertheless, further studies should be carried out to elucidate underlying molecular mechanisms and pathological process of sepsis.

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“…Slit2/robo1 pathway promotes tumor growth and metastasis [9] . Studies have shown that miR-27a-3p/Slit2 can regulate apoptosis, autophagy, and inflammatory response in LPS-treated vascular endothelial cells [10] . Autophagy and apoptosis play important roles in tumor treatment.…”

Section: Introductionmentioning

confidence: 99%

Oxymatrine Induces Autophagy and Apoptosis in Human Poorly Differentiated Gastric Adenocarcinoma Cells via Slit2/Robo1 Signals

Zhang

et al. 2021

Preprint

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Oxymatrine (OMT), is a natural quinoxaline alkaloid from the traditional Chinese medicine herb and has been shown to exhibit anticancer properties on various types of cancer cells. Poorly differentiated gastric adenocarcinoma is a common malignancy of gastric cancer that is more aggressive and has a poor prognosis. In the present study, we investigate the effects of Slit2/Robo1 signals in poorly differentiated gastric adenocarcinoma and adjacent tissues, and the anticancer properties of OMT on human poorly differentiated gastric adenocarcinoma BGC-823 cells and evaluate their underlying mechanisms. The expression levels of Slit2 and Robo1 proteins were measured in 20 pairs of human poorly differentiated gastric adenocarcinoma tissues and adjacent normal tissues using western blot. The expression of apoptosis related proteins and autophagy-related proteins was detected by western blot. The cells viability was detected by CCK-8 assay. The migration of BGC-823 cells was detected by transwell experiments. The expression of related proteins was detected by western blot. The result shows that Slit2 and Robo1 are significantly increased in poorly differentiated gastric adenocarcinoma. The apoptosis and autophagy are inhibited in poorly differentiated gastric adenocarcinoma. OMT inhibits the growth and migration of BGC-823 cells in vitro. OMT inhibits the activation of Slit2/Robo1 signals and induces apoptosis and autophagy in BGC-823 cells. These findings suggest that the antitumor effects of OMT may be the result of inhibition of cell growth and migration, and inhibits the activation of Slit2/Robo1 signals pathway and induces apoptosis and autophagy. OMT may represent a novel anticancer therapy for the treatment of poorly differentiated gastric adenocarcinoma.

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TUG1 Represses Apoptosis, Autophagy, and Inflammatory Response by Regulating miR-27a-3p/SLIT2 in Lipopolysaccharide-Treated Vascular Endothelial Cells

Cited by 17 publications

References 40 publications

MiR-27a-3p promotes the osteogenic differentiation by activating CRY2/ERK1/2 axis

MiR-27a-3p promotes the osteogenic differentiation by activating CRY2/ERK1/2 axis

Long Non-Coding RNAs as Biomarkers and Therapeutic Targets in Sepsis

Oxymatrine Induces Autophagy and Apoptosis in Human Poorly Differentiated Gastric Adenocarcinoma Cells via Slit2/Robo1 Signals

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