Tumor Ablation Enhancement by Combining Radiofrequency Ablation and Irreversible Electroporation: An In Vitro 3D Tumor Study

Zhang, Bing; Yang, Ye; Ding, Lujia; Möser, Michael; Zhang, Edwin M.; Zhang, Wenjun

doi:10.1007/s10439-018-02185-x

Cited by 21 publications

(14 citation statements)

References 43 publications

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“…The number subscript denotes the concentration of cationic polymer solutions in DW; for instance, COS DW 10 means the concentration of COS solution is 10 mg/mL; three different concentrations of cationic solutions (i.e., COS DW 10 , COS DW 50 , and COS DW 100 ) were used in the study. The fourelectrode systems used to measure electrical conductivity included: (1) a custom-made four-electrode device [27], (2) a function generator (AFG3022C, Tektronix, Beaverton, Oregon, USA) supplying a 500 kHz and 0.1-3 mA alternating current, (3) an oscilloscope (MDO 3032, Tektronix, Beaverton, Oregon, USA) measuring the current via a current probe (CT-2 current probe, Tektronix, Beaverton, Oregon, USA) and the voltage via a voltage probe (TPP0500B, Tektronix, Beaverton, Oregon, USA), and (4) a software for data collection and calculation A total of six New Zealand rabbits (2.8-3.4 kg) with three lobes each were used in the measurement, and each lobe of rabbit livers was used once or twice depending on the size of lobes. At least 0.5 mL of cationic polymer solution at each concentration was injected into the liver lobe to ensure that the area of interest was well infiltrated with solution.…”

Section: A Measurement Of Electrical Conductivitymentioning

confidence: 99%

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A Novel Method to Increase Tumor Ablation Zones With RFA by Injecting the Cationic Polymer Solution to Tissues: In Vivo and Computational Studies

Fang

Möser

Zhang

et al. 2020

IEEE Trans. Biomed. Eng.

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This study aims to examine, for the first time, the introduction of cationic polymer solutions to improve radiofrequency ablation (RFA) in terms of a potentially enlarged ablation zone. Methods: By using in vivo and computational RFA studies, two cationic polymers, Chitooligosaccharides (COS) and carboxymethyl chitosan (CMC), diluted in deionized water, were injected into tissues separately surrounding the RF bipolar electrode prior to power application. A total of 9 rabbits were used to 1) measure the increase in electrical conductivity of tissues injected with the cationic polymer solutions, and 2) explore the enhancement of the ablation performance in RFA trials. A computer model of RFA comprising a model of the solution diffusion with an RF thermal ablation model was also built, validated by the in vivo experiment, to quantitatively study the effect of cationic polymer solutions on ablation performances. Results: Compared to the control group, the electrical conductivity of rabbit liver tissues was increased by 42.20% (0.282 ± 0.006 vs. 0.401 ± 0.048 S/m, P = 0.001) and 43.97% (0.282 ± 0.006 vs. 0.406 ± 0.042 S/m, P = 0.001) by injecting the COS and CMC solution at the concentration of 100 mg/mL into the tissues, denoted COS D W 100 and CMC D W 100 , respectively. Consequently, the in vivo experiments show that the ablation zone was enlarged by 95% (47.6 ± 6.3 vs. 92.6 ± 11.5 mm 2 , P <

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Section: A Measurement Of Electrical Conductivitymentioning

confidence: 99%

“…The electrical conductivity of rabbit liver tissues injected with COS or CMC solution was measured in vivo using the four-electrode method in this study [27]. A three-dimensional computer model validated by the in vivo experiment was used to study the improved performance of RFA with the cationic polymer solutions.…”

Section: Introductionmentioning

confidence: 99%

A Novel Method to Increase Tumor Ablation Zones With RFA by Injecting the Cationic Polymer Solution to Tissues: In Vivo and Computational Studies

Fang

Möser

Zhang

et al. 2020

IEEE Trans. Biomed. Eng.

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“…High-frequency irreversible electroporation (H-FIRE) has been widely investigated to be an alternative to the conventional irreversible electroporation (IRE) because of its ability to avoid or reduce neuro and muscle contractions during the administration of bipolar electric pulses. Conventional IRE protocols deliver a series of 50-100 ls monopolar square pulses, and a common protocol in use clinically involves 90 pulses with each having a width of 100 ls, delivered at the frequency of around 1 Hz [1,2]. H-FIRE replaces the 100-ls pulses in IRE with the bursts of bipolar pulses with much smaller pulse widths (0.25-50 ls).…”

Section: Introductionmentioning

confidence: 99%

An in vivo study of a custom-made high-frequency irreversible electroporation generator on different tissues for clinically relevant ablation zones

Zhang

Liu

Fang

et al. 2021

International Journal of Hyperthermia

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Purpose: To examine the ablation zone, muscle contractions, and temperature increases in both rabbit liver and kidney models in vivo for a custom-made high-frequency irreversible electroporation (H-FIRE) generator. Materials and Methods: A total of 18 New Zealand white rabbits were used to investigate five H-FIRE protocols (n ¼ 3 for each protocol) and an IRE protocol (n ¼ 3) for the performance of the designed H-FIRE device in both liver and kidney tissues. The ablation zone was determined by using histological analysis 72 h after treatment. The extent of muscle contractions and temperature change during the application of pulse energy were measured by a commercial accelerometer attached to animals and fiber optic temperature probe inserted into organs with IRE electrodes, respectively. Results: All H-FIRE protocols were able to generate visible ablation zones without muscle contractions, for both liver and kidney tissues. The area of ablation zone generated in H-FIRE pulse protocols (e.g., 0.3-1 ls, 2000 V, and 90-195 bursts) appears similar to that of IRE protocol (100 ls, 1000 V, and 90 pulses) in both liver and kidney tissues. No significant temperature increase was noticed except for the protocol with the highest pulse energy (e.g., 1 ls, 2000 V, and 180 bursts). Conclusion: Our work serves to complement the current H-FIRE pulse waveforms, which can be optimized to significantly improve the quality of ablation zone in terms of precision for liver and kidney tumors in clinical setting.

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“…20 This technique has been researched for the treatment of cancer in situations where the use of chemotherapy is not possible. 10,34 Despite obtaining several experimental results so far, the mathematical and physical modeling of the phenomena involved still proves to be somewhat complex. This is due to the opening time of the pores being in the order of microseconds and the size of the nanometer pores, in addition to the fact that biological tissues have intense dielectric dispersion.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Electroporation Model Evaluation on Rabbit Tissues

et al. 2021

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Tumor Ablation Enhancement by Combining Radiofrequency Ablation and Irreversible Electroporation: An In Vitro 3D Tumor Study

Cited by 21 publications

References 43 publications

A Novel Method to Increase Tumor Ablation Zones With RFA by Injecting the Cationic Polymer Solution to Tissues: In Vivo and Computational Studies

A Novel Method to Increase Tumor Ablation Zones With RFA by Injecting the Cationic Polymer Solution to Tissues: In Vivo and Computational Studies

An in vivo study of a custom-made high-frequency irreversible electroporation generator on different tissues for clinically relevant ablation zones

Dynamic Electroporation Model Evaluation on Rabbit Tissues

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