Tumor–Antigen Activated Dendritic Cell Membrane-Coated Biomimetic Nanoparticles with Orchestrating Immune Responses Promote Therapeutic Efficacy against Glioma

Abstract

Immunotherapy has had a profound positive effect on certain types of cancer but has not improved the outcomes of glioma because of the blood−brain barrier (BBB) and immunosuppressive tumor microenvironment. In this study, we developed an activated mature dendritic cell membrane (aDCM)-coated nanoplatform, rapamycin (RAPA)loaded poly(lactic-co-glycolic acid) (PLGA), named aDCM@ PLGA/RAPA, which is a simple, efficient, and individualized strategy to cross the BBB and improve the immune microenvironment precisely. In vitro cells uptake and the transwell BBB model revealed that the aDCM@PLGA/RAPA can enhance homotypic-targeting and BBB-crossing efficiently. According to the in vitro and in vivo immune response efficacy of aDCM@PLGA/RAPA, the immature dendritic cells (DCs) could be stimulated into the matured status, which leads to further activation of immune cells, such as tumor-infiltrating T cells and natural killer cells, and can induce the subsequent immune responses through direct and indirect way. The aDCM@PLGA/RAPA treatment can not only inhibit glioma growth significantly but also has favorable potential ability to induce glial differentiation in the orthotopic glioma. Moreover, the aDCM@PLGA could induce a robust CD8 + effector and therefore suppress orthotopic glioma growth in a prophylactic setup, which indicates certain tumor immunity. Overall, our work provides an effective antiglioma drug delivery system which has great potential for tumor combination immunotherapy.

Covalent Organic Framework‐Based Nanomotor for Multimodal Cancer Photo‐Theranostics

“…To camouflage PPy@COF-Por, we harvested HCT116 cancer cell membranes from HCT116 cells using reported methods. [30] By mixing PPy@COF-Por with freshly harvested HCT116 cancer cell membranes in an ultrasonic bath (800 W, 40 kHz) for 30 min, mPPy@COF-Por was obtained. To verify the successful encapsulation of PPy@COF-Por by the cell membranes, we labeled the as-prepared mPPy@COF-Por with a cell membrane staining dye (DiO dye).…”

Nanostructures in Chinese herbal medicines (CHMs) for potential therapy

“…Polymer nanoparticles can facilitate drug transport across the BBB and are often used to study brain diseases. [134][135][136][137] For the effective treatment of PD, biodegradable polyethylene glycol-B-polytrimethylene carbonate nanoparticles are developed to promote the sustained release of Ginkgolide B for 48 h, and to prevent neuronal cytotoxicity. 57 Some studies have found that CUR-encapsulated PLGA nanoparticles (Cur-PLGA-NPs) can induce adult neurogenesis by activating the classical Wnt/b-catenin pathway, and Cur-PLGA-NPs may treat neurodegenerative diseases, such as Alzheimer's disease (AD), by enhancing the self-repair mechanism of the brain.…”

Advanced Generation Therapeutics: Biomimetic Nanodelivery System for Tumor Immunotherapy

“…DCs activation, aDCM@PLGA/RAPA preparation, and its immunotherapy effect with direct or indirect approaches. Reprinted with permission under a Creative Commons license from ref . Copyright (2023) American Chemical Society.…”

“…Moreover, long-term antitumor protection of DCMs-coated SiO 2 NPs was demonstrated in the B16 tumor model. Furthermore, Ma et al developed an activation mature DCMs nanoplatforms to package poly­(lactic- co -glycolic acid) (PLGA) loaded with rapamycin (RAPA), which can effectively enhance homologous targeting ability and blood–brain barrier (BBB) permeability (Figure ). The results confirm that immature DCs can be stimulated to the mature state to further activate tumor-infiltrating T cells and NK cells and to induce subsequent immune responses through direct or indirect approaches.…”