Tumor Cell Respiration Following Irradiation

Abstract

Oxygen consumption rates for mouse mastocytoma cells examined from 3 to 4 hours after x-irradiation with 250-2000 rads were consistently higher than controls. Cyclic fluctuations in oxygen consumption per cell were dose-dependent; peaks in consumption were generally greater in magnitude and occurred later in time with increasing dose. The cyclic response in consumption is probably due to cell synchronization effects, while the overall higher respiration rates of irradiated cells may reflect increased metabolic activity in response to radiation damage. Oxygen consumption rates for the total cell population were cyclic after 250 and 500 rads, but declined steadily after 1000 or 2000 rads; oxygen consumption rates tended to be lower than controls, especially 12-24 hours postirradiation. Differences between cellular and population oxygen consumption rates reflect changes in cell number after irradiation. It is suggested that postirradiation reoxygenation kinetics in solid tumors may result from changes in tumor oxygen demand.

Endothelial cell death after ionizing radiation does not impair vascular structure in mouse tumor models

“…Those patients with persistent hypoxia had poorer outcomes, suggesting that patients with persistent tumor hypoxia might benefit from more intensive treatments (Zips et al , 2012; Lock et al , 2017, 2019). One possible explanation for tumor hypoxia is reduced perfusion, although other explanations could account for increased oxygenation (Good & Harrington, 2013) including changes in the oxygen consumption rate (Clement et al , 1978; Ashton et al , 2016; Gallez et al , 2017). Some tumors were found to have higher levels of perfusion at intermediate times in a course of radiation therapy, but in other cases perfusion remained unchanged for at least several weeks in both cervical and in head and neck cancers (Shibuya et al , 2011; Lock et al , 2017).…”