Tumor development in rats and cancer cachexia are reduced by treatment with botryosphaeran by increasing apoptosis and improving the metabolic profile

Geraldelli, Danielli; Ribeiro, Mariana Costa; Medeiros, Tulio Couto; Comiran, Patrícia K.; Martins, Kamila Ortega; Oliveira, Matheus Fernandes de; Oliveira, Gabriela Araújo; Dekker, Robert F. H.; Barbosa-Dekker, Aneli M.; Alegranci, Pâmela; Queiroz, Eveline Aparecida Isquierdo Fonseca de

doi:10.1016/j.lfs.2020.117608

Cited by 17 publications

(19 citation statements)

References 41 publications

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Section: Methodsmentioning

confidence: 99%

“…The tumour cells were used for the inoculation in the rats, and the formation of solid tumour, in the fourth passage of the cells from the successive transplants. The maintenance of the Walker-256 cancer cells in the rats and the tumour cell inoculation protocol was followed according to that outlined by Geraldelli et al 23 The animals from the CTB and OTB groups received treatment with botryosphaeran (30 mg/kg b.w., $5 mL/rat/d) once daily via gavage over 15 days from the 9th to 11th week of the experiment.…”

Section: Animals and Experimental Protocolmentioning

confidence: 99%

“…18 Previous studies from our research group have additionally demonstrated that botryosphaeran has significant anti-obesogenic activity, 19 anti-proliferative and proapoptotic activity against breast carcinoma cells (MCF-7), 20 and antitumour activity in Walker-256 tumour-bearing obese and non-obese rats. [21][22][23] Geraldelli et al 22,23 demonstrated that botryosphaeran, at a dose of 30 mg/kg/d for 15 days, presented a significant antitumour and anti-cachetic effect in Walker-256 tumour-bearing obese and nonobese rats. The mechanism associated, at least in part, is with the beneficial metabolic effects of botryosphaeran in reducing the accumulation of adipose tissue and improving insulin sensitivity, as well as increasing apoptosis of tumour cells.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant, anti‐inflammatory and beneficial metabolic effects of botryosphaeran [(1→3)(1→6)‐β‐d‐glucan] are responsible for its anti‐tumour activity in experimental non‐obese and obese rats bearing Walker‐256 tumours

Silva

Geraldelli

Martins

et al. 2022

Cell Biochemistry & Function

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Botryosphaeran, a (1!3)(1!6)-β-D-glucan, presents several beneficial activities, such as antiproliferative, hypoglycemic and antitumoural activities. This study evaluated the effects of botryosphaeran on oxidative stress, inflammation and metabolic activities in Walker-256 tumour-bearing non-obese and obese rats. Wistars rats were divided into four groups: control tumour (CT); control tumour + botryosphaeran (CTB); obese tumour (OT), and obese tumour + botryosphaeran (OTB). In ninth week, obese and non-obese rats were inoculated with 1 Â 10 7 Walker-256 tumour cells and treated with botryosphaeran (30 mg/kg/d for 15 days). In 11th week, the following parameters were evaluated glycogen, glucose and lactate levels, pro-oxidant (TBARS) and antioxidant markers (superoxide dismutase [SOD]; catalase [CAT]; glutathione-S-transferase [GST]; reduced glutathione [GSH]; vitamin C) and cytokines. Obesity presented oxidative stress and inflammation, as demonstrated by high levels of TBARS, SOD and TNF-α, and lower levels of CAT, GSH and interleukin-10 (IL-10). Botryosphaeran significantly decreased TBARS and TNF-α and increased GST, GSH, vitamin C and IL-10 in the liver; increased SOD and vitamin C in tumour tissue; decreased TBARS in adipose tissue, and notably decreased the levels of glycogen and lactate in the tumour of CTB rats. Botryosphaeran promoted significant antioxidant, anti-inflammatory, and beneficial metabolic effects in Walker-256 tumour-bearing non-obese and obese rats, which contributed to its antitumour activity.

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Section: Methodsmentioning

confidence: 99%

Section: Animals and Experimental Protocolmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antioxidant, anti‐inflammatory and beneficial metabolic effects of botryosphaeran [(1→3)(1→6)‐β‐d‐glucan] are responsible for its anti‐tumour activity in experimental non‐obese and obese rats bearing Walker‐256 tumours

Silva

Geraldelli

Martins

et al. 2022

Cell Biochemistry & Function

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“…Comiran et al (2021) constatou que o tratamento utilizando botriosferana na dose de 12 mg/kg/dia, por 15 dias, tanto em ratos obesos quanto em não obesos que apresentavam o tumor de Walker-256, foi capaz de atenuar o desenvolvimento tumoral e a síndrome da caquexia do câncer nos dois grupos (obesos e não obesos). O mecanismo relacionado aos efeitos metabólicos e imunológicos da botriosferana consistem na correção da resistência à insulina e hiperglicemia, modulação das concentrações plasmáticas de colesterol e aumento da contagem de leucócitos e linfócitos em ratos obesos (COMIRAN et al, 2020).…”

Section: Obesidade Câncer E Classes De Medicamentos/substância Com Po...unclassified

“…Ainda, estudos recentes demonstraram que a botriosferana, na dose de 30 mg/kg/dia, por 15 dias, apresenta um importante efeito antitumoral e anticaquético em animais obesos e não obesos com tumor de Walker-256, e que isso está associado à melhora dos parâmetros metabólicos e hematológicos, reduzindo o acúmulo de tecido adiposo, aumentando a sensibilidade à insulina, corrigindo o perfil glicídico e lipídico e a anemia macrocítica (GERALDELLI et al, 2020a e b). Além disso, a botriosferana foi eficaz em promover aumento da apoptose das células neoplásicas (GERALDELLI et al, 2020a) e aumento da atividade do FOXO3a (Forkhead transcription factor 3a), fator de transcrição supressor tumoral que contribui para a parada do ciclo celular e para o aumento da apoptose das células tumorais (GERALDELLI et al, 2020b) Além de diminuir o desenvolvimento de tumores por melhorar o perfil metabólico e a resposta imune de quem a utiliza, a botriosferana também aumenta a atividade de certos genes supressores tumorais, como o FOXO3a (SILVA et al, 2018;GERALDELLI et al, 2020). Ao fazer isso, o medicamento estimula a produção de proteínas por esses genes, que irão inibir processos de proliferação celular e impedir o avanço e/ou o desenvolvimento de neoplasias.…”

Section: Obesidade Câncer E Classes De Medicamentos/substância Com Po...unclassified

Obesidade e câncer: mecanismos envolvidos e intervenções terapêuticas

et al. 2022

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A transição demográfica iniciada no Brasil, a partir da década de 60, promoveu mudanças nos hábitos de vida da população, promovendo alterações no perfil nutricional e nas manifestações de doenças. No início do século XXI, ocorreu um marco no qual, pela primeira vez, o número de indivíduos com excesso de peso ultrapassou os de baixo peso, o que é preocupante, pois a obesidade é apontada como um dos principais fatores de risco para inúmeras patologias crônicas, incluindo diabetes, dislipidemia, doenças cardiovasculares e inúmeros tipos de câncer. A incidência e a mortalidade por câncer vêm aumentando no mundo, em parte devido ao envelhecimento e crescimento populacional, mas também pela mudança da distribuição e prevalência dos fatores de risco relacionados ao câncer. Vários fatores como mudanças metabólicas ou hormonais, influenciadas pelo aumento do tecido adiposo, resistência à insulina, hiperinsulinemia, inflamação crônica de baixo grau, maior biodisponibilidade de hormônios e estresse oxidativo, contribuem para os mecanismos de carcinogênese na obesidade. A alimentação balanceada, além de contribuir para o controle de peso, pode exercer o papel protetor em relação à carcinogênese. Há pesquisas em andamento que avaliam o potencial de vegetais verdes-escuros, alaranjados, crucíferos (repolho, brócolis, couve de Bruxelas), produtos de soja, feijão (carioca, preto, branco, vermelho), legumes, cebola, alho e tomate na prevenção do câncer. Outro fator importante é a prática regular de atividade física, que diminui o risco de câncer por melhorar a sensibilidade à insulina, o que estimula a imunidade e diminui os níveis de adipocina e marcadores inflamatórios. Dessa forma, esta revisão tem como objetivo analisar e relacionar informações associadas com a obesidade e o câncer.

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Oral administration of botryosphaeran [(1 → 3)(1 → 6)‐β‐d‐glucan] reduces inflammation through modulation of leukocytes and has limited effect on inflammatory nociception

Silva

Pereira

Santos

et al. 2022

Cell Biochemistry & Function

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Several biological activities of the fungal exopolysaccharide (1 → 3)(1 → 6) -β-D-glucan (botryosphaeran) have been described in the literature, but its effects on inflammation have not been evaluated. This study aimed to investigate the action of botryosphaeran on experimental mice models of carrageenan-induced acute pleurisy and acute paw edema, and complete Freund's adjuvant-induced persistent paw edema. All botryosphaeran doses tested (1.0, 2.5, 5.0, and 10.0 mg/kg birth weight [b.w.], orally administered) reduced leukocyte recruitment, nitric oxide (NO) levels, and protein extravasation in the pleural cavity. Botryosphaeran (5 mg/kg b.w.) did not diminish edema and mechanical hyperalgesia in the paw within 4 h; however, cold allodynia was alleviated within the first 2 h. In the persistent paw inflammation model, the effects of daily oral administration of botryosphaeran (5 mg/kg b.w.) were evaluated over 3 and 7 days. The fungal β-glucan significantly reduced the levels of the cytokines, tumor necrosis factor(TNF)-α, interleukin (IL)-6), and IL-10, in the paw homogenates in both protocols, while paw edema and the levels of advanced oxidation protein products (AOPP) only diminished on Day 7. No effect in mechanical hyperalgesia was observed. Oral treatment for 3 or 7 days also decreased the plasma levels of NO, AOPP, TNF-α, and IL-10. On Day 7, the number of leukocytes in the blood was also reduced by this treatment. Importantly, botryosphaeran did not induce inflammation in mice when administered alone over 7 days. This study demonstrated the anti-inflammatory and antinociceptive potential of botryosphaeran in these experimental models, making this fungal β-glucan a new possibility for complementary treating acute and chronic inflammation.

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Tumor development in rats and cancer cachexia are reduced by treatment with botryosphaeran by increasing apoptosis and improving the metabolic profile

Cited by 17 publications

References 41 publications

Antioxidant, anti‐inflammatory and beneficial metabolic effects of botryosphaeran [(1→3)(1→6)‐β‐d‐glucan] are responsible for its anti‐tumour activity in experimental non‐obese and obese rats bearing Walker‐256 tumours

Antioxidant, anti‐inflammatory and beneficial metabolic effects of botryosphaeran [(1→3)(1→6)‐β‐d‐glucan] are responsible for its anti‐tumour activity in experimental non‐obese and obese rats bearing Walker‐256 tumours

Obesidade e câncer: mecanismos envolvidos e intervenções terapêuticas

Oral administration of botryosphaeran [(1 → 3)(1 → 6)‐β‐d‐glucan] reduces inflammation through modulation of leukocytes and has limited effect on inflammatory nociception

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