Tumor homing indocyanine green encapsulated micelles for near infrared and photoacoustic imaging of tumors

Uthaman, Saji; Bom, Joon-suk; Kim, Hyeon Sik; John, Johnson V.; Bom, Hee‐Seung; Kim, Seon-Jong; Min, Jung‐Joon; Kim, Il; Park, In Kyu

doi:10.1002/jbm.b.33607

Cited by 20 publications

(16 citation statements)

References 40 publications

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“…For the first time, the potential application of free ICG as cell labeling agent and PA tracer was explored. As already done by Uthaman et al [42], the PA visualization of the cell engraftment was paralleled by NIRF acquisition in order to perform a comparative study of the two imaging modalities. Despite the minor toxicity and the related advantages for in vivo applications, ICG exhibits very complex optical properties.…”

Section: Discussionmentioning

confidence: 99%

“…Remarkably, in these conditions, the exposure to the contrast agent did not produce any relevant alteration in the cell profile, suggesting that ICGlabeled MSCs may retain their therapeutic efficacy. Finally, when it comes to in vivo use of the ICG, the results interpretation is further complicated by the intricacy of molecular interactions with the various components of the biological environment [25,42]. Here, the PA behavior of the dye was investigated in complex circumstances involving concentrationdependent effects, internalization by cells, interactions with cell components and deposition into living tissues.…”

Section: Discussionmentioning

confidence: 99%

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Indocyanine green labeling for optical and photoacoustic imaging of mesenchymal stem cells after in vivo transplantation

Filippi

Garello

Pasquino

et al. 2019

Journal of Biophotonics

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The transplantation of mesenchymal stem cells (MSCs) holds great promise for the treatment of a plethora of human diseases, but new noninvasive procedures are needed to monitor the cell fate in vivo. Already largely used in medical diagnostics, the fluorescent dye indocyanine green (ICG) is an established dye to track limited numbers of cells by optical imaging (OI), but it can also be visualized by photoacoustic imaging (PAI), which provides a higher spatial resolution than pure near infrared fluorescence imaging (NIRF). Because of its successful use in clinical and preclinical examinations, we chose ICG as PAI cell labeling agent. Optimal incubation conditions were defined for an efficient and clinically translatable MSC labeling protocol, such that no cytotoxicity or alterations of the phenotypic profile were observed, and a consistent intracellular uptake of the molecule was achieved. Suspensions of ICG‐labeled cells were both optically and optoacoustically detected in vitro, revealing a certain variability in the photoacoustic spectra acquired by varying the excitation wavelength from 680 to 970 nm. Intramuscular engraftments of ICG‐labeled MSCs were clearly visualized by both PAI and NIRF over few days after transplantation in the hindlimb of healthy mice, suggesting that the proposed technique retains a considerable potential in the field of transplantation‐focused research and therapy. Stem cells were labeled with the Food and Drug Administration (FDA)‐approved fluorescent dye ICG, and detected by both PAI and OI, enabling to monitor the cell fate safely, in dual modality, and with good sensitivity and improved spatial resolution.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Indocyanine green labeling for optical and photoacoustic imaging of mesenchymal stem cells after in vivo transplantation

Filippi

Garello

Pasquino

et al. 2019

Journal of Biophotonics

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“…This allows both passive targeting (by the EPR effect due to nanoparticles) and active targeting (due to the affinity of hyaluronic acid for CD44). Uthaman et al70 hydrophobically modified ICG prior to loading it into hyaluronic acid-based micelles and utilized these nanoparticles for CD44-based targeting. They used hyaluronic acid-C18 (octadecylamine) micelles, explaining that this type of micelle is better than the systems in which ICG is conjugated on the polymer.…”

Section: Icg Nanoparticles For Active Tumor Targetingmentioning

confidence: 99%

<p>NIR fluorescence-guided tumor surgery: new strategies for the use of indocyanine green</p>

Egloff-Juras¹,

Bezdetnaya²,

Dolivet³

et al. 2019

IJN

150

111

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Surgery is the frontline treatment for a large number of cancers. The objective of these excisional surgeries is the complete removal of the primary tumor with sufficient safety margins. Removal of the entire tumor is essential to improve the chances of a full recovery. To help surgeons achieve this objective, near-infrared fluorescence-guided surgical techniques are of great interest. The concomitant use of fluorescence and indocyanine green (ICG) has proved effective in the identification and characterization of tumors. Moreover, ICG is authorized by the Food and Drug Administration and the European Medicines Agency and is therefore the subject of a large number of studies. ICG is one of the most commonly used fluorophores in near-infrared fluorescence-guided techniques. However, it also has some disadvantages, such as limited photostability, a moderate fluorescence quantum yield, a high plasma protein binding rate, and undesired aggregation in aqueous solution. In addition, ICG does not specifically target tumor cells. One way to exploit the capabilities of ICG while offsetting these drawbacks is to develop high-performance near-infrared nanocomplexes formulated with ICG (with high selectivity for tumors, high tumor-to-background ratios, and minimal toxicity). In this review article, we focus on recent developments in ICG complexation strategies to improve near-infrared fluorescence-guided tumor surgery. We describe targeted and nontargeted ICG nanoparticle models and ICG complexation with targeting agents.

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“…Also, fluorescent HA probes may be used in an intraoperative to assess Hyal activity or drug delivery of theranostic probes ( 119 ). Often, HA probes contain more than one CA moiety to harness the strength of multimodal imaging, such as MRI/optical imaging ( 123 , 136 ), MRI/computer tomography (CT) ( 137 ), NIRF/CT ( 138 ), or NIRF/PA imaging ( 139 – 141 ) to improve diagnostic capability and monitoring of therapeutic efficacy ( 121 , 142 ).…”

Section: The Tumor Stroma and Its Imagingmentioning

confidence: 99%

Characterization of the Tumor Microenvironment and Tumor–Stroma Interaction by Non-invasive Preclinical Imaging

Ramamonjisoa

Ackerstaff

2017

Front. Oncol.

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Tumors are often characterized by hypoxia, vascular abnormalities, low extracellular pH, increased interstitial fluid pressure, altered choline-phospholipid metabolism, and aerobic glycolysis (Warburg effect). The impact of these tumor characteristics has been investigated extensively in the context of tumor development, progression, and treatment response, resulting in a number of non-invasive imaging biomarkers. More recent evidence suggests that cancer cells undergo metabolic reprograming, beyond aerobic glycolysis, in the course of tumor development and progression. The resulting altered metabolic content in tumors has the ability to affect cell signaling and block cellular differentiation. Additional emerging evidence reveals that the interaction between tumor and stroma cells can alter tumor metabolism (leading to metabolic reprograming) as well as tumor growth and vascular features. This review will summarize previous and current preclinical, non-invasive, multimodal imaging efforts to characterize the tumor microenvironment, including its stromal components and understand tumor–stroma interaction in cancer development, progression, and treatment response.

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Tumor homing indocyanine green encapsulated micelles for near infrared and photoacoustic imaging of tumors

Cited by 20 publications

References 40 publications

Indocyanine green labeling for optical and photoacoustic imaging of mesenchymal stem cells after in vivo transplantation

Indocyanine green labeling for optical and photoacoustic imaging of mesenchymal stem cells after in vivo transplantation

<p>NIR fluorescence-guided tumor surgery: new strategies for the use of indocyanine green</p>

Characterization of the Tumor Microenvironment and Tumor–Stroma Interaction by Non-invasive Preclinical Imaging

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