Tumor Regression Induced in Mice by Partially Purified Lymphokine Fractions*

Abstract

Over the past six years our studies have involved the use of cell-free supernatants derived from long term cultures of human and mouse lymphoblast cellThe properties of lymphocyte mediators (lymphokines) have been extensively reviewed 4 9 and a recent workshop summary on this subject covers basic and clinical aspects.'j Human peripheral blood lymphocytes have proved to be rich sources of lymphokine-containing supernatants and have been used for most studies on in vitro lymphokine properties. We have used lymphoblast cell lines as sources of starting material rather than antigen or lectin-stimulated peripheral blood lymphocytes. The purpose has been solely to obtain large quantities of starting material for use in animal studies, preclinical and clinical toxicity studies, standardizing tests for biological properties, and biochemical characterization. In all of these studies, large quantities of starting materials from the same lot are required in order to compare effects on a regular basis. In spite of using the same cell line, variations in the biological properties of supernatants from lot to lot have provided some difficulties in the preparation and purification of materials, as well as biological assay. Early laboratory and clinical studies have been published and I report here our more recent mouse tumor assay experiments and some of the B-cell stimulation studies performed by Drs. Butler and Specter in Dr. Herman Friedman's laboratory.Methodology for the establishment and maintenance of human lymphoid cell lines has been described,' as well as the preparation of supernatant lympho-*

Degradation of a chromogenic substrate by α2‐macroglobulin from plasma of patients with rheumatoid arthritis

“…A similar PBA can be generated in high density rabbit lymphocyte cultures ( 5 ) or in the serum of rabbits inoculated intravenously with allogeneic lymphoid cells (6). Also, lymphokine activity has been found to be associated with a2M in supernatants of the normal human lymphoblastoid cell line RPMI 1788 (7). Recently, it has been shown that intraarticular injection of a2M-protease complexes into rabbits results in histopathologic changes that suggest similarities to those observed in RA patients (8).…”

Lymphotoxin: An anticarcinogenic lymphokine as measured by inhibition of chemical carcinogen or ultraviolet‐irradiation‐induced transformation of syrian hamster cells

“…The properties and functions of lymphokines, including those exhibiting cytotoxic activity, have been recently reviewed (Cohen et al, 1979). Lymphotoxin, a lymphokine produced by mitogen or antigenstimulated-lymphocytes, inhibits tumor cell growth in vivo May-Levin et al, 1972;Papermaster et al, 1974Papermaster et al, , 1976Papermaster et al, , 1979 and in vitro (Gately et al, 1976;Rosenberg et al, 1973;Sawada et al, 1976;Williams and Granger, 1973). Furthermore, lymphotoxin is frequently many times more effective in vitro as a growth inhibitor of a variety of tumor cells than of non-tumorigenic cells from the same species (Evans et al, 1975;Meltzer and Bartlett, 1972;Rundell and Evans, 1981;Weedon et al, 1973).…”