Tumor‐Specific Multipath Nucleic Acid Damages Strategy by Symbiosed Nanozyme@Enzyme with Synergistic Self‐Cyclic Catalysis

Abstract

The high proliferation efficiency, redox imbalance, and elevated nucleic acid repair capabilities of tumor cells severely restrict the theranostic efficacy. Selectively interference chaotic tumors with devastating nucleic acid damages (NUDs) properties are expected to overcome theranostic barriers. Here, an exquisite catalytic‐based strategy with comprehensive NUDs mechanisms is demonstrated. In this regard, enzyme (glucose oxidase, GOD) symbioses nanozyme Cu3+x(PO4)2 through biomineralization (abbreviated as Cu@GOD), GOD can disorder the metabolism by consuming glucose, thereby inhibiting the nutrition supply for nucleic acid repair. GOD‐catalyzed H2O2 guarantees the self‐cyclic glutathione depletion and reactive oxygen species generation caused by Cu3+x(PO4)2, resulted the reduced antioxidation defense and enhanced oxidation assault, ensures an indiscriminate NUDs ability. Moreover, the high photothermal effect of Cu3+x(PO4)2 induces effective tumor inhibition. Consequently, this substantial multipath NUDs strategy, with potentials of suppressing the cytoprotective mechanisms, amplifying the cellular oxidative stress, and disrupting the redox balance to ensure substantial irreversible NUDs, completely breaks the obstacle of chaotic tumors, providing new conceptual thinking for tumor proliferation inhibition.

A biomineralized bi-functional hybrid nanoflower to effectively combat bacteriaviaa glucose-powered cascade catalytic reaction

“…According to the XPS spectra, Cu-GMP/GODNF contained 70.85% Cu 2+ and 29.15% Cu + , indicating the coordination of Cu with N/O atoms of GOD/GMP. 36,37 The FT-IR spectra showed the characteristic peaks of GMP and GOD in the Cu-GMP/GODNF (amino region, 1712–1489 cm −1 and 1427–1369 cm −1 ) (Fig. 1K).…”

“…According to the XPS spectra, Cu-GMP/GODNF contained 70.85% Cu 2+ and 29.15% Cu + , indicating the coordination of Cu with N/O atoms of GOD/GMP. 36,37 The FT-IR spectra showed the characteristic peaks of GMP and GOD in the Cu-GMP/GODNF (amino region, 1712-1489 cm À1 and Scheme 1 Synthesis route of Cu-GMP/GODNF nanozymes (A) and schematic illustration of chemodynamic sterilization through in situ generation of ROS on the surface of bacteria by cascade catalytic reactions (B). 1427-1369 cm À1 ) (Fig.…”

Metal-fluorouracil networks with disruption of mitochondrion enhanced ferroptosis for synergistic immune activation

“…5-Fu drugs as chemotherapeutic drug can disturb DNA transcription and Zinc is able to disrupt the redox balance, which would act the synergistically damage to DNA in tumor cells. Therefore, we investigated the DNA damage induced by Zn 2+ , Fu or Zn-Fu MNs, via γ-H 2 AX staining (Figure 3 I) 53 . From confocal images, those cancer cells treated with only Zn 2+ or Fu showed the obvious γ-H 2 AX stained fluorescence signals, in contrast with PBS group, which indicated the disturbed DNA transcription.…”

Cascade-Enhanced Catalytic Nanocomposite with Glutathione Depletion and Respiration Inhibition for Effective Starving-Chemodynamic Therapy Against Hypoxic Tumor

“…7,8 Thus, GOx was recently explored to be co-delivered with Fenton/Fenton-like agents to realize cascade catalysis. [9][10][11] Moreover, the glucose-consuming feature of GOx can perturb energy metabolism in tumor cells, fulfilling synergistic starvation therapy (ST). 12 Nevertheless, this GOxmediated catalytic process is O 2 -reliant and would be limited by hypoxic microenvironment in solid tumors.…”

“…19 Due to its excellent performance in protecting the bioactivity of enzymes and pH-responsive drug release characteristic, 20 biomineralization has recently inspired a series of biomimetic synthesis strategies to form GOx-loaded nanoparticles (NPs) for synergistic ST and CDT. 9,11,21 For instance, in suit mineralization was performed to synthesize GOx/copperdoped calcium phosphate (CaP). 21 Once at the tumor site, the decomposition of CaP will be triggered by the acidic microenvironment to release GOx and Cu 2+ , following which GSH was consumed and •OH was generated during the two-way conversion between Cu 2+ and Cu + .…”