Tumor Suppressor Gene p16/INK4A/CDKN2A and Its Role in Cell Cycle Exit, Differentiation, and Determination of Cell Fate

Agarwal, Payal; Kabir, Farruk M. Lutful; DeInnocentes, Patricia; Bird, Richard M.

doi:10.5772/27882

Cited by 16 publications

(17 citation statements)

References 185 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We have shown abundant expression of HOXA10 in the same CMT cell lines harbouring putative SIRT2 defects . Surprisingly, no HOX genes, except the SIX1 gene, were identified among those over or underexpressed transcripts investigated by DNA array analysis (Affymetrix Gene Chip Canine Genome 2.0 Array) in canine mammary cancers although other common tumour suppressor gene defects in canine mammary cancer also were not detected. Possible sources for this absence of any reported HOX gene expression include use of primary canine breast cancer biopsies instead of cell lines with up to 30% normal cells present in biopsy specimens, selection of only metastatic tumours, and/or a complex patient breed structure.…”

Section: Discussionmentioning

confidence: 81%

“…We have previously identified several tumour suppressor and oncogenes that appear to be frequently and similarly dysfunctional in human and canine breast cancer cells. These include the oncogene c‐ erb B‐2/HER‐2, the tumour suppressor genes p16/INK4A, p14ARF, p53, and p21/Cip1 and the gene silencing histone deacetylase gene encoded by canine SIRT2 . Others have investigated canine hematopoietic stem cells where HOXB3 and HOXA10 were more highly expressed in CD34+ stem cell‐enriched cell fractions .…”

Section: Discussionmentioning

confidence: 99%

“…Yet it is also clear that HOX genes have an important and continuing role to play even in adult and especially older animals as dysfunction, in at least some of these genes, has been directly associated with neoplastic potential in humans . Our recent efforts have identified genetic defects, among some of the known canine tumour suppressor and oncogenes, contributing to spontaneous neoplastic transformation in a highly similar canine model of human breast cancer . Little is currently known regarding the contributions of canine HOX genes to spontaneous mammary neoplasia in dogs …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization ofHOXgene expression in canine mammary tumour cell lines from spontaneous tumours

DeInnocentes

Perry

Graff

et al. 2013

Vet Comparative Oncology

View full text Add to dashboard Cite

Spatial/temporal controls of development are regulated by the homeotic (HOX) gene complex and require integration with oncogenes and tumour suppressors regulating cell cycle exit. Spontaneously derived neoplastic canine mammary carcinoma cell models were investigated to determine if HOX expression profiles were associated with neoplasia as HOX genes promote neoplastic potential in human cancers. Comparative assessment of human and canine breast cancer expression profiles revealed remarkable similarity for all four paralogous HOX gene clusters and several unlinked HOX genes. Five canine HOX genes were overexpressed with expression profiles consistent with oncogene-like character (HOXA1, HOXA13, HOXD4, HOXD9 and SIX1) and three HOX genes with underexpressed profiles (HOXA11, HOXC8 and HOXC9) were also identified as was an apparent nonsense mutation in HOXC6. This data, as well as a comparative analysis of similar data from human breast cancers suggested expression of selected HOX genes in canine mammary carcinoma could be contributing to the neoplastic phenotype.

show abstract

Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization ofHOXgene expression in canine mammary tumour cell lines from spontaneous tumours

DeInnocentes

Perry

Graff

et al. 2013

Vet Comparative Oncology

View full text Add to dashboard Cite

show abstract

“…p16 inhibits CDK4/6-mediated phosphorylation of Rb and leads to the incompetent pRb-E2F complex and finally inhibits the cell cycle [7]. p16 is one of the most common tumour suppressor genes in glioma, and various mutations and deletions contribute to the cause of cancer [8]. However, its role as a prognostic marker was not explored until the discovery of BMI1, which is a key stem cell factor.…”

Section: Introductionmentioning

confidence: 98%

CDKN2A (p16) mRNA decreased expression is a marker of poor prognosis in malignant high-grade glioma

et al. 2015

View full text Add to dashboard Cite

Human high-grade glioma is heterogeneous in nature based on pathological and genetic profiling. Various tumour suppressor gene alterations are considered as prognostic markers in high-grade glioma. Gene expression of CDKN2A (p16) is used in various cancers as a prognostic biomarker along with methylation and deletion status of this gene. Expression levels of p16 mRNA were not studied as a biomarker in gliomas before. In this study, we have performed mRNA quantification analysis on 48 high-grade glioma tissues and checked for a possible prognostic role. The decreased expression of p16 mRNA in majority of the tumour tissues (57.1 %) was observed when compared to control tissues (P = 0.02). mRNA expression level was correlated with clinical variables also. p16 deletion status and BMI1 mRNA expression were also considered for comparison. p16 mRNA was negatively correlated with the BMI1 mRNA (P = <0.0001) but not with p16 deletion. p16 mRNA expression, midline shift in MRI and tumour type were able to predict patient survival in overall survival (OS) and progression-free survival (PFS). p16 mRNA could independently predict prognosis of OS (P = 0.0146) and PFS (P = 0.0305) in multivariate analysis. We have shown that p16 mRNA expression can act as an independent prognostic biomarker in high-grade glioma.

show abstract

“…Hambatan fosforilasi Rb menyebabkan pelepasan faktor transkripsi E2F dari kompleks-kompleks Rb/E2F.Akibatnya, protein terakumulasi dalam nukleus dan sitoplasma dan dapat dideteksi oleh imunohistokimia. 22 Ekspresi p16 sering diamati pada HPV risiko tinggi dibandingkan lesi pada risiko rendah.Ekspresi imunohistokimia p16 umumnya terkait dengan HPV onkogenik.Pewarnaan nuclear dan sitoplasma yang difus dan kuat biasanya berkorelasi dengan infeksi HPV risiko tinggi.…”

unclassified

Imunohistokimia Pada Kondilomata Akuminata

Widyaswari

Lumintang

Soemarno

2019

MHSJ

View full text Add to dashboard Cite

Background: Condylomata Acuminata (CA) is a sexually transmitted disease caused by the Human Papilloma Virus (HPV) type-specific and may be simultaneously co-infected with other HPV types associated with malignancy. Purpose: To differentiate between CA and histopathological of malignancy by immunohistochemistry. LiteratureReview: CA refers to benign epidermal proliferation caused by the HPV types 6 and 11, but co-infection with highrisk HPV types are common. The clinical presentation of CA can't differentiate between benign or preneoplastik diplasia lesions. Koilocytes considered pathognomonic for HPV lesions, these findings sometimes don't appear on CA, and histopathology was not accurate, that immunohistochemistry with MIB-1 (Ki-67) and p16 can beused. Conclusion: Immunohistochemistry examination can help differentiate the diagnosis between malignancy or nonmalignancy in the case of CA. ABSTRAKLatar Belakang: Kondilomata Akuminata (KA) merupakan penyakit menular seksual yang disebabkan Human Papilloma Virus (HPV) tipe tertentu dan mungkin dapat terinfeksi secara bersamaan dengan tipe HPV lain yang terkait dengan keganasan. Tujuan: untuk membedakan gambaran histopatologi antara KA dan keganasan dengan pemeriksaan imunohistokimia. Telaah Kepustakaan: KA mengacu pada proliferasi epidermal jinak yang disebabkan HPV tipe 6 dan 11, tapi ko-infeksi dengan HPV tipe risiko tinggi sering terjadi. Gambaran klinis pada KA tidak dapat membedakan lesi jinak dari preneoplastik atau lesi diplastik. Koilositosis dianggap patognomonik lesi HPV, temuan ini kadang-kadang tidak tampak pada KA, dan histopatologi tidak tepat, sehingga dapat menggunakan pemeriksaan imunohistokimia dengan biomarker MIB-1(ki-67) dan p16. Kesimpulan: Pemeriksaaan imunohistokimia dapat membantu mendiagnosis banding pada kasus KA yang samar-samar maupun keganasan.

show abstract

Tumor Suppressor Gene p16/INK4A/CDKN2A and Its Role in Cell Cycle Exit, Differentiation, and Determination of Cell Fate

Cited by 16 publications

References 185 publications

Characterization ofHOXgene expression in canine mammary tumour cell lines from spontaneous tumours

Characterization ofHOXgene expression in canine mammary tumour cell lines from spontaneous tumours

CDKN2A (p16) mRNA decreased expression is a marker of poor prognosis in malignant high-grade glioma

Imunohistokimia Pada Kondilomata Akuminata

Contact Info

Product

Resources

About