Tumor-targeting and pH-sensitive lipoprotein-mimic nanocarrier for targeted intracellular delivery of paclitaxel

In situ preparation of water-soluble ginsenoside Rh2-entrapped bovine serum albumin nanoparticles: in vitro cytocompatibility studies

“…Further, in analysis of pH-dependent stability, the NPs degradation was observed by measuring the particle size at 37°C in PBS (pH 7.4) as a function of time ( Figure 7B). 10 Conversely, NP size was increased at pH 5.0 in acidic conditions, primarily due to the hydrolysis of Rh2 from BSA-Rh2 NPs, resulting in the formation of large aggregates of the hydrolyzed Rh2 through hydrophobic interaction. The most noteworthy finding from our stability analyses was the high stability of BSA-Rh2 NPs in physiological buffer, which indicated that the NPs may exhibit prolonged circulation in vivo.…”

“…9 The high abundance of this plasma protein in the human body and its small size and stability in pH range from 4 to 9, and temperature up to 60°C, for approximately 10 hours, make it a suitable carrier for transporting numerous therapeutic drugs in various human cell lines. 10 Additionally, these protein-based drug delivery nanocarriers have the intrinsic property of preferential uptake by tumor and inflamed tissues. Additionally, they show little or no toxicity and immunogenicity, and are not biodegradable.…”

“…10,34 Albumin-based nanocarriers are designated as drug delivery carrier materials as albumin is the most abundant blood protein, nontoxic and biocompatible in nature, and has abundant surface functional groups, all of which are quite helpful for drug loading or entrapment and binding of various biological molecules. 12 Importantly, serum proteins naturally found in the blood are chiefly responsible for the maintenance of blood pH, and are nontoxic, biodegradable, and nonimmunogenic in nature.…”

Synthesis and characterization of a PAMAM-OH derivative containing an acid-labile β-thiopropionate bond for gene delivery

“…MTT assay was performed according to the report by Chen and colleagues (Chen et al, 2015). Briefly, cells were seeded at a density of 1×10 4 cells/well in 96-well plates and incubated in 100 μl fresh medium for 24 h. Then polyplexes with 200 ng plasmid were added to each well and the cells were further incubated for 48 h. 10 µl 12 freshly prepared MTT in PBS (5 mg/ml) was added to each well.…”

An ultrasensitive LC–MS/MS method with liquid phase extraction to determine paclitaxel in both cell culture medium and lysate promising quantification of drug nanocarriers release in vitro

“…Since PTx release kinetic is time and medium composition depending, a highly sensitive quantification of drug at short time is required to validate the use of such nanocarriers for drug delivery in cancer therapy. Indeed, due to its high hydrophobicity, most of in vitro studies of PTx release from nanocarriers were performed in simulated physiological medium supplemented with surfactant Tween 80 [11][12][13][14][15][16][17][18]. Numerous analytical methods using liquid phase extraction and solid phase extraction combined to the LC-MS/MS technique were developed for ultrasensitive quantifi- cation of PTx in biological samples such as cells, plasma, urine, feces or tissues [19][20][21][22][23][24][25][26][27][28][29][30].…”