Tumorhead distribution to cytoplasmic membrane of neural plate cells is positively regulated by Xenopus p21-activated kinase 1 (X-PAK1)

Wu, Cong; Delsert, Claude; Faure, Sandrine; Traverso, Edwin E.; Kloc, Małgorzata; Kuang, Jian; Etkin, Laurence D.; Morin, Nathalie

doi:10.1016/j.ydbio.2007.05.015

Cited by 6 publications

(4 citation statements)

References 77 publications

(89 reference statements)

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“…3B). This was further supported by expression of a mutant of X-PAK1 (KR-PAK1), which acts as a dominant-negative kinase and blocks endogenous X-PAK1 activation (Wu et al, 2007). Expression of KR-PAK1 inhibited CNC cell migration.…”

Section: The Level Of Rhou Activity Is Critical For Explant Polarity mentioning

confidence: 97%

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Activity of the RhoU/Wrch1 GTPase is critical for cranial neural crest cell migration

Fort¹,

Guémar²,

Vignal³

et al. 2011

Developmental Biology

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The neural crest (NC) is a stem cell-like population that arises at the border of neural and non-neural ectoderm. During development, NC undergoes an epithelio-mesenchymal transition (EMT), i.e. loss of epithelial junctions and acquisition of pro-migratory properties, invades the entire embryo and differentiates into a wide diversity of terminal tissues. We have studied the implication of Rho pathways in NC development and previously showed that RhoV is required for cranial neural crest (CNC) cell specification. We show here that the non-canonical Wnt response rhoU/wrch1 gene, closely related to rhoV, is also expressed in CNC cells but at later stages. Using both gain- and loss-of-function experiments, we demonstrate that the level of RhoU expression is critical for CNC cell migration and subsequent differentiation into craniofacial cartilages. In in vitro cultures, RhoU activates pathways that cooperate with PAK1 and Rac1 in epithelial adhesion, cell spreading and directional cell migration. These data support the conclusion that RhoU is an essential regulator of CNC cell migration.

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Section: The Level Of Rhou Activity Is Critical For Explant Polarity mentioning

confidence: 97%

“…Xenopus Rac1 ORF was amplified by RT-PCR and cloned in pCS2+. pCS2-KR-PAK1 and pCS2-DE-PAK1 were previously described (Wu et al, 2007). Synthetic-capped mRNAs were generated using the mMessage mMachine kit (Ambion).…”

Section: Dna Constructs and Mrnas Synthesismentioning

confidence: 99%

Activity of the RhoU/Wrch1 GTPase is critical for cranial neural crest cell migration

Fort¹,

Guémar²,

Vignal³

et al. 2011

Developmental Biology

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“…Zebrafish Dnd1 was shown to block the 430/427/302 family of miRNAs in the germ plasm that target repression of nanos and TDRD7 (Tudor domain-containing 7) (BehmAnsmant et al 2006;Wu et al 2007). In Xenopus, miRNAs mediate the rapid deadenylation of maternal mRNAs after MBT as well.…”

Section: Dead-end (Dnd1)mentioning

confidence: 99%

Germ‐Cell Specification inXenopus

King

2014

Xenopus Development

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Primordial germ cells (PGCs) represent the only precursor stem cell population that normally gives rise to the gametes. For this reason, PGCs have been called the "stem cells of the species" and are prime examples of a normal totipotent state (Wylie, 1999. Cell 96 (2): 165-174). PGCs represent a remarkable lineage that will differentiate into highly specialized cells, either oocytes or sperm, yet also retain the ability to generate a complete new individual. Thus, a fundamental question in developmental and stem cell biology is how PGCs differentiate while retaining the potential for totipotency in contrast to somatic cells that become fate-restricted. In this chapter, we will review what is known about how Xenopus sets aside its germ-cell lineage.

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“…Ectopic overexpression of Tumorhead mutant localizes to the plasma membrane; this results in neural plate expansion and inhibition of neuronal differentiation, which leads to embryonic lethality. xPAK1 mediates the phosphorylation of Tumorhead and enhances its binding to the apical cortex and lateral cell membrane of neural plate epithelial cells, resulting in neural plate expansion and inhibition of neuronal differentiation; organisms without xPAK1 activity show loss of the Tumorhead phenotype [Wu et al, 2007].…”

Section: Xenopusmentioning

confidence: 99%

PAK thread from amoeba to mammals

Kumar

Molli

Pakala

et al. 2009

J of Cellular Biochemistry

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The p21-activated kinases (PAKs) are signaling nodes that play a crucial role in cellular processes including cell motility, differentiation, survival, gene transcription and hormone signaling. PAKs are highly conserved family of serine threonine kinases that act as effector for small GTPases Rac and Cdc42. Most of our knowledge about PAK functions has been derived from genetic approaches in lower organisms and many of these functions are similar to that seen in mammalian cells. In this review, we have summarized the extensive information generated in lower eukaryotes and very briefly discussed the current status of PAKs in humans.

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Tumorhead distribution to cytoplasmic membrane of neural plate cells is positively regulated by Xenopus p21-activated kinase 1 (X-PAK1)

Cited by 6 publications

References 77 publications

Activity of the RhoU/Wrch1 GTPase is critical for cranial neural crest cell migration

Activity of the RhoU/Wrch1 GTPase is critical for cranial neural crest cell migration

Germ‐Cell Specification inXenopus

PAK thread from amoeba to mammals

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