Tumour necrosis factor, IL-1 and IL-6 in bronchoalveolar washings and their<i>in vitro</i>production during<i>Nippostrongylus brasiliensis</i>infection

Benbernou, N.; Matsiota-Bernard, P.; Jolivet, C.; Ougen, P.; Guenounou, Moncef

doi:10.1111/j.1365-2249.1992.tb03071.x

Cited by 12 publications

(1 citation statement)

References 21 publications

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“…We have shown previously that TNF‐α was not detected during a primary infection with N. brasiliensis (7). A large amount of TNF‐α activity is shared with interleukin‐1 (IL‐1) and transient IL‐1 production has been observed in N. brasiliensis infected rats (31). Some parasitic helminth derived products have been demonstrated to have a potent anti‐inflammatory activity by suppressing pro‐inflammatory cytokine production.…”

Section: Discussionmentioning

confidence: 99%

Effect of Nippostrongylus brasiliensis L3 ES on inflammatory mediator gene transcription in lipopolysaccharide lung inflammation

Zhao

Brown

MacCallum

et al. 2008

Parasite Immunology

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The anti-inflammatory properties of parasitic helminths have been largely linked to their excretory-secretory (ES) products. Some studies have noted a lack of TNF-alpha production and limited recruitment of neutrophils into the lungs after Nippostrongylus brasiliensis infection. We previously reported that instillation of ES from L3 larvae of N. brasiliensis to the lungs could inhibit the recruitment of neutrophils on a background of LPS-induced inflammation. A similar reduction in neutrophil recruitment was observed in this study. This reduction was associated with the significant inhibition in gene transcription of the adhesion molecule, ICAM-1, and the chemokine, MIP-2 in bronchoalveolar lavage (BAL) cells. The LPS-stimulated gene transcription of the pro-inflammatory cytokines TNF-alpha and IL-1beta was also significantly reduced by L3 ES. Inducible nitric oxide synthase (iNOS) is normally elevated in classically activated macrophages, however, in this case gene transcription of iNOS was inhibited by L3 ES and may suggest a phenotype change to anti-inflammatory. The general inhibition of pro-inflammatory mediators observed in this study suggests that infective stage L3 larvae excrete and/or secrete inhibitory products capable of modifying the normally potent LPS inflammatory response.

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Section: Discussionmentioning

confidence: 99%

Effect of Nippostrongylus brasiliensis L3 ES on inflammatory mediator gene transcription in lipopolysaccharide lung inflammation

Zhao

Brown

MacCallum

et al. 2008

Parasite Immunology

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Exogenous surfactant decreases oxygenation inEscherichia coli endotoxin-treated neonatal piglets

et al. 1996

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Abnormalities of pulmonary surfactant function have been described in association with the acute respiratory distress syndrome (ARDS). Because gram‐negative sepsis is a common cause of ARDS, we treated neonatal piglets with Escherichia coli endotoxin to create a neonatal ARDS model. We hypothesized that under these conditions administration of exogenous surfactant would improve pulmonary function. Study groups included: control (n = 8), Exosurfk (5 mL/kg. 13.5 mg phospholipid/mL, n = 7), Survantak (4 mL/kg. 25 mg phospholipid/mL, n = 6), and saline (5 mL/kg, n = 6). E. coli endotoxin 12 μg/kg was infused over 30 min and resulted in significant pulmonary and hemodynamic abnormalities, histopathologic evidence of nonhomogeneous lung injury, and elevated protein levels in bronchoalveolar lavage washings. Neither Exosurfk nor Survantak ameliorated the pulmonary effects of endotoxin. Instead, there was a prolonged decrease in arterial oxygen tension (PaO2) and dynamic lung compliance after administration of surfactant and saline. Distribution of a bolus of Exosurfk was uneven throughout the lung. We conclude that in this neonatal piglet model of ARDS, bolus surfactant administration had a detrimental effect on oxygenation and pulmonary function. Pediatr Pulmonol. 1996; 22:376–386. © 1996 Wiley‐Liss, Inc.

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Antisense oligonucleotides to interleukin‐4 regulate IgE and IgG2a production by spleen cells from Nippostrongylus brasiliensis‐infected rats

Benbernou¹,

Matsiota-Bernard²,

Guenounou³

1993

Eur J Immunol

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Interleukin-4 (IL-4) plays a key role in the regulation of immunoglobulin production. In the present study, we examined the role of IL-4 in the production of IgE and IgG2a by spleen cells from Nippostrongylus brasiliensis-infected rats using the antisense oligonucleotide strategy. Three antisense oligonucleotides (15 and 20 nucleotides) were selected near the ATG initiation codon of the murine IL-4 gene. Significant and reproducible inhibitions of IgE and IgG2a production were observed with the addition of two different oligonucleotides, the most inhibitory being specific for 15 nucleotides corresponding to the downstream AUG sequence oligodeoxynucleotide (ODN-1), while the corresponding sense sequence had no effect. The inhibition was directly related to the ODN-1 concentration on IgE response, and IgG2a production was more sensitive to the inhibitory effect of ODN-1 than the IgE response. Our results suggest that the ODN-1 effect is rather specific for the IL-4 gene since the production of other cytokines was not modified and the exogenous addition of IL-4 reversed the ODN-1 effect. In contrast to IgE and IgG2a, ODN-1 induced a significant enhancement of IgM synthesis. The use of a phosphorothioate ODN of the same sequence as ODN-1 resulted in a greater inhibitory effect on IgE and IgG2a production. These data indicate that IL-4 contributes to the spontaneous production of IgE and IgG2a, and argue that antisense strategy constitutes a useful tool for the regulation of antibody response.

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Tumour necrosis factor, IL-1 and IL-6 in bronchoalveolar washings and theirin vitroproduction duringNippostrongylus brasiliensisinfection

Cited by 12 publications

References 21 publications

Effect of Nippostrongylus brasiliensis L3 ES on inflammatory mediator gene transcription in lipopolysaccharide lung inflammation

Effect of Nippostrongylus brasiliensis L3 ES on inflammatory mediator gene transcription in lipopolysaccharide lung inflammation

Exogenous surfactant decreases oxygenation inEscherichia coli endotoxin-treated neonatal piglets

Antisense oligonucleotides to interleukin‐4 regulate IgE and IgG2a production by spleen cells from Nippostrongylus brasiliensis‐infected rats

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