Tumour-suppressive microRNA-424-5p directly targets CCNE1 as potential prognostic markers in epithelial ovarian cancer

Liu, Jingjing; Gu, Zhenpeng; Tang, Yujie; Hao, Junmei; Zhang, Cuiping; Yang, Xinggang

doi:10.1080/15384101.2017.1407894

Cited by 51 publications

(33 citation statements)

References 36 publications

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“…There is a growing consensus that miR-424 exerts a suppressive effect on cell proliferation and cell cycle progression (Liu et al, 2018;Pande et al, 2018;Wang J. et al, 2018). Nonetheless, some studies have indicated that miR-424 could promote cell differentiation by targeting the cell cycle regulator, Cdc25A, to enhance cell cycle arrest (Sarkar et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

miR-424 Promotes Bovine Adipogenesis Through an Unconventional Post-Transcriptional Regulation of STK11

Wang

Zhang

et al. 2020

Front. Genet.

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Adipose tissue is the largest energy reservoir and secretory organ in the animal body, and is essential for maintaining normal physiological functions and metabolic balance. MicroRNAs regulate the process of adipogenic differentiation through posttranscriptional regulatory mechanisms. In the present study, miR-424 was upregulated during bovine adipocyte differentiation both in vivo and in vitro. The overexpression and interference of miR-424 exhibited the positive regulatory role in the differentiation of bovine adipocytes. Furthermore, miR-424 directly binds to the three prime untranslated region (3' UTR) of serine/threonine kinase 11 (STK11, also called LKB1), a master upstream gene in the AMP-activated protein kinase (AMPK) cascade, and up-regulates its expression. Functional studies showed that the knockdown of STK11 attenuated the pro-adipogenic effect of miR-424. Post-transcriptional regulation of STK11 by miR-424 was mediated potentially in an RNA binding protein (RBP) binding site-dependent manner. In conclusion, our study shows that miR-424 promotes bovine adipogenesis through an unconventional post-transcriptional regulation of STK11, which may serve as a potential target for the regulation of bovine adipogenesis and the improvement of livestock breeding efficiency.

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Section: Discussionmentioning

confidence: 99%

miR-424 Promotes Bovine Adipogenesis Through an Unconventional Post-Transcriptional Regulation of STK11

Wang

Zhang

et al. 2020

Front. Genet.

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“…A recent study has suggested that miR-424-5p functions as a tumor suppressor in breast cancer [ 40 ]. MiR-424-5p inhibits CCNE1 expression (encoding cyclin E1) in ovarian cancer, and inhibits cell cycle by suppressing the E2F transcription factor 1 (E2F1)-RB transcriptional corepressor (PRb) pathway, which promotes apoptosis [ 41 ]. Progression of malignant tumors, such as colon carcinoma, epithelial ovarian cancer, and cervical cancer involves miR-424-5p [ 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA AGAP2-AS1 increases the invasiveness of papillary thyroid cancer

Shao

Sun

Zhang

et al. 2020

Aging

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Papillary thyroid cancer (PTC) is considered a low hazard endocrine system cancer, but a considerable number of patients have poor prognosis because of lymph node metastasis and invasion of surrounding tissues. In this study, we analyzed the expression and function of the long non-coding RNA (lncRNA) AGAP2-AS1 in PTC. We found that AGAP2-AS1 expression was significantly higher in human PTC tissues than adjacent noncancerous tissues (n=110; p<0.01) and correlated with lymph node metastasis (p=0.01) and tumor-node-metastasis stage (p=0.006). AGAP2-AS1 downregulation decreased migration and invasion by PTC cells, and reduced expression of matrix metalloproteinase-2 (MMP2). AGAP2-AS 1 upregulated MMP2 expression by competitively binding to microRNA-425-5p. In addition, miR-424-5p expression was decreased in PTC tissues and correlates negatively with the AGAP2-AS1 levels. These results demonstrate that AGAP2-AS1 expression is significantly elevated in PTC tissues and that, by binding to miRNA-425-5p, it upregulates the MMP2 expression, thereby increasing the invasiveness and migration capacity of PTC cells.

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“…Our ndings uncover the signi cance of the interaction between MYLK-AS1 and miR-424-5p in tumorigenesis given that MYLK-AS1 exerts oncogenic behavior partly via sponging miR-424-5p in HCC cells. In addition, miR-424-5p functions as a tumor suppressor and directly targets CCNE1 as potential prognostic markers in epithelial ovarian cancer [25] .…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of Competitive Endogenous Rnas Network Associated With Hepatocellular Carcinoma

Wan

Wang

Zhao

et al. 2020

Preprint

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Background: Increasing evidences show that long non-coding RNA (lncRNA) plays the role of competitive endogenous RNAs (ceRNAs) in the development and progression of cancers. The purpose of our study was to identify potential lncRNA biomarkers that serve as a therapeutic target and prognostic biomarker in HCC.Methods: The differential expression of RNAs was examined using the edgeR package. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were used to predict potential functions. Survival analysis and ROC curve analysis were performed to predict ceRNA network had significant prognostic value. The biological functions of MYLK-AS1 on HCC cells were studied by RNA interference approaches in vitro. Cell proliferation, migration and invasion were detected by Cell Counting Kit-8(CCK-8) assay, wound healing and transwell assay relatively. The mechanism of competitive endogenous RNAs (ceRNAs) were predicted and verified by bioinformatic analysis, western blot analysis and luciferase assays.Results: The newly constructed ceRNA network comprised 76 HCC specific lncRNAs, 15 miRNAs, and 35 mRNAs from the database (Targetscan, miRTarBase, and miRDB). 10 differentially expressed lncRNAs and 10 differentially expressed mRNAs were significantly associated with overall survival in HCC (P value < 0.05). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathways enrichment analysis results showed the differentially expressed mRNAs were involved primarily in the most significant cancer-related signaling pathways. ROC curve analysis demonstrated that MYLK-AS1—has-mir-424—CCNE1 ceRNA network had significant prognostic value. The expression of MYLK-AS1 was up-regulated in HCC cells and tissues. Biological function analyses indicated that down-regulation of MYLK-AS1 suppressed cell proliferation, migration and invasion . MYLK-AS1 and miR-424-5p bound directly and reversibly to each other. MYLK-AS1 could positively regulate CCNE1 expression by competitively binding to miR-424-5p. Conclusion: The current study provides novel insights into the lncRNA-related ceRNA network in HCC and the MYLK-AS1 may be a candidate biomarker for molecular diagnosis and prognosis monitoring of HCC.

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Tumour-suppressive microRNA-424-5p directly targets CCNE1 as potential prognostic markers in epithelial ovarian cancer

Cited by 51 publications

References 36 publications

miR-424 Promotes Bovine Adipogenesis Through an Unconventional Post-Transcriptional Regulation of STK11

miR-424 Promotes Bovine Adipogenesis Through an Unconventional Post-Transcriptional Regulation of STK11

Long non-coding RNA AGAP2-AS1 increases the invasiveness of papillary thyroid cancer

Comprehensive Analysis of Competitive Endogenous Rnas Network Associated With Hepatocellular Carcinoma

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