Tunable-frequency three-dimensional structured illumination microscopy with reduced data-acquisition

Doblas, Ana; Shabani, Hasti; Saavedra, Genaro; Preza, Chrysanthe

doi:10.1364/oe.26.030476

Cited by 8 publications

(1 citation statement)

References 29 publications

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“…For this reason, many microscopy techniques have been developed to obtain 3D information. Many of them rely on capturing multiple images [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ], which makes them inappropriate for moving samples and dynamic processes. Lightfield microscopy is an emerging technique that solves this problem thanks to its capacity to capture three-dimensional information in a single shot.…”

Section: Introductionmentioning

confidence: 99%

The Lightfield Microscope Eyepiece

Incardona

Tolosa

Scrofani

et al. 2021

Sensors

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Lightfield microscopy has raised growing interest in the last few years. Its ability to get three-dimensional information about the sample in a single shot makes it suitable for many applications in which time resolution is fundamental. In this paper we present a novel device, which is capable of converting any conventional microscope into a lightfield microscope. Based on the Fourier integral microscope concept, we designed the lightfield microscope eyepiece. This is coupled to the eyepiece port, to let the user exploit all the host microscope’s components (objective turret, illumination systems, translation stage, etc.) and get a 3D reconstruction of the sample. After the optical design, a proof-of-concept device was built with off-the-shelf optomechanical components. Here, its optical performances are demonstrated, which show good matching with the theoretical ones. Then, the pictures of different samples taken with the lightfield eyepiece are shown, along with the corresponding reconstructions. We demonstrated the functioning of the lightfield eyepiece and lay the foundation for the development of a commercial device that works with any microscope.

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