Tunable, low-repetition-rate, cost-efficient femtosecond Ti:sapphire laser for nonlinear microscopy

Antal, P.; Szipöcs, R.

doi:10.1007/s00340-011-4830-7

Cited by 29 publications

(17 citation statements)

References 15 publications

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“…The CARS technique presented here can be used for in vivo label‐free 3D visualization of lipid bilayers, lipid droplets, neuronal myelin sheets and small metabolites for instance without any labeling. Existing limitations on the imaging depth of the CARS system presented can be overcome by using lower repetition rate laser sources (Antal and Szipocs, ), longer excitation laser wavelengths and/or THG imaging method of lipids (Liao et al, ), which can pave the way for in vivo diagnostics of obesity related structural changes of the skin.…”

Section: Discussionmentioning

confidence: 99%

In vivosecond-harmonic generation andex vivocoherent anti-stokes raman scattering microscopy to study the effect of obesity to fibroblast cell function using an Yb-fiber laser-based CARS extension unit

Haluszka

Lőrincz

Molnár

et al. 2015

Microsc. Res. Tech.

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Nonlinear microscopy techniques are being increasingly used to perform in vivo studies in dermatology. These methods enable us to investigate the morphology and monitor the physiological process in the skin by the use of femtosecond lasers operating in the red, near-infrared spectral range (680-1,300 nm). In this work we used two different techniques that require no labeling: second harmonic generation (SHG) for collagen detection and coherent anti-Stokes Raman scattering (CARS) to assess lipid distribution in genetically obese murine skin. Obesity is one of the most serious public health problems due to its high and increasing prevalence and the associated risk of type 2 diabetes and cardiovascular diseases. Other than these diseases, nearly half of patients with diabetes mellitus suffer from dermatological complications such as delayed wound healing, foot ulcers and several other skin changes. In our experiment we investigated and followed the effects of obesity on dermal collagen alterations and adipocyte enlargement using a technique not reported in the literature so far. Our results indicate that the in vivo SHG and ex vivo CARS imaging technique might be an important tool for diagnosis of diabetes-related skin disorders in the near future.

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Section: Discussionmentioning

confidence: 99%

In vivosecond-harmonic generation andex vivocoherent anti-stokes raman scattering microscopy to study the effect of obesity to fibroblast cell function using an Yb-fiber laser-based CARS extension unit

Haluszka

Lőrincz

Molnár

et al. 2015

Microsc. Res. Tech.

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show abstract

“…Ultrashort pulse lasers with high peak power and high stability have found wide applications in the fields of laser measurement, laser spectrum, nonlinear optics, biological medicine, micromachining process, and so on [1][2][3][4]. The pulse width and the repetition rate are the very important parameters in short pulse lasers because they decide the dynamical process of the interreaction between laser and materials.…”

Section: Introductionmentioning

confidence: 99%

Numerical simulation of low repetition rate subnanosecond laser based on dual-loss-modulation

Zhao

Yang

et al. 2013

Appl. Opt.

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A set of coupled rate equations for diode-pumped Q-switched and mode-locked laser with electro-optic (EO) modulator and middle semiconductor saturable absorber mirror under the Gaussian spatial distribution approximation are given. The numerically simulated results of these equations show that the pulse width of the Q-switched envelope are related to the repetition rate of EO, the stimulated emission section of the gain medium, the pump power and so on. When the pulse width of the Q-switched envelope is shorter than the cavity roundtrip transmit time, i.e., the interval of two neighboring mode-locking pulses, there is only one mode-locked pulse lying in a Q-switched envelope and its repetition rate depends on that of EO. This means that single mode-locking pulses with low repetition rate, subnanosecond duration, high peak power, and high stability are generated. The simulated results are consistent with the experimental values.

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“…An increased commercial demand for low PRF systems in fields like biomedical photonics, chemistry, and, nonlinear microscopy in the recent years has certainly fuelled interest within the laser research community [18,19]. Achieving turn-key systems with low repetition rates combined with high peak and average output power, wide-tunability and short pulse duration, will support such systems to become an alternative to the conventionally used Ti:Sa laser technologies operating at a PRF as low as 80 MHz [20] in these fields. Also, VECSELs are known to have a better cost to wavelength flexibility figure of merit compared to the Ti:Sa lasers currently used.…”

Section: Introductionmentioning

confidence: 99%

Mapping the dynamical regimes of a SESAM mode-locked VECSEL with a long cavity using time series analysis

et al. 2018

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The different dynamical regions of an optically-pumped SESAM mode-locked, long-cavity VECSEL system with a fundamental pulse repetition frequency of ~200 MHz are investigated. The output power, captured as 250 μs long time series using a sampling rate of 200 GSa/s, for each operating condition of the system, is analyzed to determine the dynamical state. A wavelength range of 985-995 nm and optical pump powers of 10 W-16.3 W is studied. The system produces high quality fundamental passive mode-locking (FML) over an extensive part of the parameter space, but the different dynamical regions outside of FML are the primary focus of this study. We report five types of output: CW emission, FML, mode-locking of a few modes, double pulsing, and, semi-stable 4 harmonic mode-locking. The high sampling rate of the oscilloscope, combined with the long duration of the time series analyzed, enables insight into how the structure and substructure of pulses vary systematically over thousands of round trips of the laser cavity. Higher average output power is obtained in regions characterized by semi-stable 4 harmonic mode-locking than observed for FML, raising whether such average powers might be achieved for FML. The observed dynamic transitions from fundamental mode-locking provide insights into instability challenges in developing a stable, widely tunable, low repetition rate, turn-key system; and to inform future modelling of the system.

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Tunable, low-repetition-rate, cost-efficient femtosecond Ti:sapphire laser for nonlinear microscopy

Cited by 29 publications

References 15 publications

In vivosecond-harmonic generation andex vivocoherent anti-stokes raman scattering microscopy to study the effect of obesity to fibroblast cell function using an Yb-fiber laser-based CARS extension unit

In vivosecond-harmonic generation andex vivocoherent anti-stokes raman scattering microscopy to study the effect of obesity to fibroblast cell function using an Yb-fiber laser-based CARS extension unit

Numerical simulation of low repetition rate subnanosecond laser based on dual-loss-modulation

Mapping the dynamical regimes of a SESAM mode-locked VECSEL with a long cavity using time series analysis

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