Tuning of nanoparticle biological functionality through controlled surface chemistry and characterisation at the bioconjugated nanoparticle surface

Hristov, Delyan R.; Rocks, Louise; Kelly, Philip M.; Thomas, Steffi S.; Pitek, Andrzej S.; Verderio, Paolo; Mahon, Eugene; Dawson, Kenneth A.

doi:10.1038/srep17040

Cited by 56 publications

(70 citation statements)

References 21 publications

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“…The dye conjugate was previously prepared by mixing 5 mg of uorescein isothiocyanate (FITC) with 2.5 mL of ethanol and 25 mL of APTES, following a procedure described in the literature. 57,58…”

Section: Nanoparticle Synthesismentioning

confidence: 99%

Enhancing curcumin's solubility and antibiofilm activityviasilica surface modification

et al. 2020

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Section: Nanoparticle Synthesismentioning

confidence: 99%

Enhancing curcumin's solubility and antibiofilm activityviasilica surface modification

et al. 2020

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“…An additional complication is provided by the presence of surface accessible and inaccessible ligands for some nanomaterials [17]. Distinguishing between these ligands is particularly important in cases where the modified nanomaterial is used for further functionalization, as for example to add biotargeting ligands [18].…”

Section: Introductionmentioning

confidence: 99%

A Multi-Method Approach for Quantification of Surface Coatings on Commercial Zinc Oxide Nanomaterials

et al. 2020

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Surface functionalization is a key factor for determining the performance of nanomaterials in a range of applications and their fate when released to the environment. Nevertheless, it is still relatively rare that surface groups or coatings are quantified using methods that have been carefully optimized and validated with a multi-method approach. We have quantified the surface groups on a set of commercial ZnO nanoparticles modified with three different reagents ((3-aminopropyl)-triethoxysilane, caprylsilane and stearic acid). This study used thermogravimetric analysis (TGA) with Fourier transform infrared spectroscopy (FT-IR) of evolved gases and quantitative solution 1H nuclear magnetic resonance (NMR) for quantification purposes with 13C-solid state NMR and X-ray photoelectron spectroscopy to confirm assignments. Unmodified materials from the same suppliers were examined to assess possible impurities and corrections. The results demonstrate that there are significant mass losses from the unmodified samples which are attributed to surface carbonates or residual materials from the synthetic procedure used. The surface modified materials show a characteristic loss of functional group between 300–600 °C as confirmed by analysis of FT-IR spectra and comparison to NMR data obtained after quantitative release/extraction of the functional group from the surface. The agreement between NMR and TGA estimates for surface loading is reasonably good for cases where the functional group accounts for a relatively large fraction of the sample mass (e.g., large groups or high loading). In other cases TGA does not have sufficient sensitivity for quantitative analysis, particularly when contaminants contribute to the TGA mass loss. X-ray photoelectron spectroscopy and solid state NMR for selected samples provide support for the assignment of both the functional groups and some impurities. The level of surface group loading varies significantly with supplier and even for different batches or sizes of nanoparticles from the same supplier. These results highlight the importance of developing reliable methods to detect and quantify surface functional groups and the importance of a multi-method approach.

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“…PEG H: 7x10 -2 PEG/nm 2 ; PEG M: 4x10 -2 PEG/nm 2 ; PEG L: 3.1x10 -3 PEG/nm 2 .The dispersions were left to react in this way for one hour after which they were washed four times in the same manner described above. Information on PEG density was done following the method described in 43 . Details are available in the SI.…”

Section: Pegylation Of Bare Silicamentioning

confidence: 99%

“…Furthermore, in the context of nanomedicine, the combined measurements of several physicochemical properties of a material (e.g. initial size and composition 40 , shape, charge 40 , surface functional groups 41 , the presence, type and density of ligands 42,43 , possible impurities, etc.) are of interest because of their synergistic influence on NP "identity".…”

Section: Introductionmentioning

confidence: 99%

Using single nanoparticle tracking obtained by nanophotonic force microscopy to simultaneously characterize nanoparticle size distribution and nanoparticle–surface interactions

Hristov

Araújo

et al. 2017

Nanoscale

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Comprehensive characterization of nanomaterials for medical applications is a challenging and complex task due to the multitude of parameters which need to be taken into consideration in a broad range of conditions. Routine methods such as dynamic light scattering or nanoparticle tracking analysis provide some insight into the physicochemical properties of particle dispersions. For nanomedicine applications the information they supply can be of limited use. For this reason, there is a need for new methodologies and instruments that can provide additional data on nanoparticle properties such as their interactions with surfaces. Nanophotonic force microscopy has been shown as a viable method for measuring the force between surfaces and individual particles in the nano-size range. Here we outline a further application of this technique to measure the size of single particles and based on these measurement build the distribution of a sample. We demonstrate its efficacy by comparing the size distribution obtained with nanophotonic force microscopy to established instruments, such as dynamic light scattering and differential centrifugal sedimentation. Our results were in good agreement to those observed with all other instruments. Furthermore, we demonstrate that the methodology developed in this work can be used to study complex particle mixtures and the surface alteration of materials. For all cases studied, we were able to obtain both the size and the interaction potential of the particles with a surface in a single measurement. IntroductionNanotechnology for medical applications has been a topic of much scientific interest for several decades due to its potential to address existing challenges in patient treatment 1,2 . In particular, the use of nanoparticles (NPs) as components in the design of targeted drug delivery has been an exciting concept. The field is now facing serious challenges partly due to the reduced circulation lifetime of NPs compared to more conventional approaches [3][4][5] . One of the main obstacles is that as NPs enter into living organisms they adsorb biomolecules forming a layer, known as the biomolecular corona. The NPbiomolecular complex has been shown to have a strong correlation with the "identity" of the materials and determine their biodistribution [6][7][8] . Mechanistic details of the interaction between this complex and cell/tissue surfaces in the body remain unclear, in part due to the lack of reliable methods to measure the physicochemical properties of materials in real exposure conditions. This includes, but is not limited to, accurate size of the particle-protein complex, NP-surface interaction at different conditions and the diffusivity of NPs close to a surface. Such information combined with other advanced characterization methods to study the accessible epitopes on the biomolecular corona could help elucidate the interaction mechanisms between NPs and the cell surface. The most commonly property used to characterize a NP dispersion is its size distribution. For biological ...

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Tuning of nanoparticle biological functionality through controlled surface chemistry and characterisation at the bioconjugated nanoparticle surface

Cited by 56 publications

References 21 publications

Enhancing curcumin's solubility and antibiofilm activityviasilica surface modification

Enhancing curcumin's solubility and antibiofilm activityviasilica surface modification

A Multi-Method Approach for Quantification of Surface Coatings on Commercial Zinc Oxide Nanomaterials

Using single nanoparticle tracking obtained by nanophotonic force microscopy to simultaneously characterize nanoparticle size distribution and nanoparticle–surface interactions

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