Turbidimetric Assay of Apolipoprotein A-II

Schriewer, H; Emke, F.; Assmann, Gerd

doi:10.1515/cclm.1985.23.6.355

Cited by 3 publications

(4 citation statements)

References 4 publications

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“…Problems involved are lack of standardized procedures, lack of reliable quality control materials, changing immunoreactivity due to self-association and aggregation of the particles, varying content of lipid which may mask epitopes, and different affinities of the antisera. Assays and assay modifications have been recently described for apoA-I (350)(351)(352)(353)(354), apoB (352,353,(355)(356)(357)(358), Lp(a) (359), apoC (353), apoC-III (360,361). Reference ranges are not yet well defined, especially those of apolipoproteins (362,363).…”

Section: Analytes Of Clinical Interestmentioning

confidence: 99%

Clinical chemistry

Stinshoff¹,

Stein²,

Wood³

et al. 1987

Anal. Chem.

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Section: Analytes Of Clinical Interestmentioning

confidence: 99%

Clinical chemistry

Stinshoff¹,

Stein²,

Wood³

et al. 1987

Anal. Chem.

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“…Despite the fact that the apolipoproteins of HDL are present in structural complexes with lipids, they can be determined quantitatively by conventional immunological techniques without further processing (8,9). Although the antigenic determinants of apolipoprotein A-II in particular are masked by the protein-lipid bond inside the HDL (10), delipidation with organic solvents is not necessary (11).…”

Section: Discussionmentioning

confidence: 99%

“…Comparison with the RID values also shows good agreement (r = 0.913). The immunoturbidimetric methods require measuring times of 12.5 minutes (9), 25 minutes (11) and 60 minutes (8), which facilitate a high sample throughput. With a measuring time of 12 minutes, the nephelometric method described is thus one of the quiekest determination procedures for apolipoprotein A-II.…”

Section: Discussionmentioning

confidence: 99%

Immunonephelometric Determination of the Apolipoprotein A-II

Häfner

Luley²,

Prellwitz³

1989

Clinical Chemistry and Laboratory Medicine

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Summary:A fully mechanized immunonephelometric method is described for the rapid and specific determination of apolipoprotein A-II in serum. The method utilizes commercially available sheep antiserum against human apolipoprotein A-II. Nephelometry was performed with the Behring Nephelometer Analyzer (BNA). A single determination can be performed in 12 minutes, requiring 10 μΐ sample volume. The measuring r nge is about 0.08 to 1.25 g/l apolipoprotein A-II. Precision is characterized by intra-assay coefficients of Variation of 3.37%, 3.93% and 4.49% for apolipoprotein A-II concentrations of 1.22 g/l, 0.376 g/I and 0.185 g/l, and inter-assay coefficients of Variation of 4.27% for an apolipoprotein A-II concentration of 0.404 g/l, respectively. Accuracy of the method is shown by the close correlation of results with those from radial immunodiffusion (r = 0.913, y = 1.091 χ -0.033, n = 75).

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“…HDL apolipoprotein A-II was measured by turbidimetry using the Cobas Bio analyser s described in detail elsewhere (17).…”

Section: Analysis Of Hdl Componentsmentioning

confidence: 99%

HDL Apolipoprotein A-I and HDL Apolipoprotein A-II Concentrations in Male Company Employees in Westphalia Aged 40 Years and Older

Schriewer¹,

Emke²,

Funke³

et al. 1986

Clinical Chemistry and Laboratory Medicine

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Summary:The major structural components of high density lipoproteins were determined in the sera of 638 male employees aged 40 years and older. It was demonstrated that the HDL apolipoprotein A-I/HDL cholesteröl ratio äs well äs the HDL apolipoprotein A-II/HDL eholesterol ratio are similarly correlated to a cumulative score of established risk factors for atherosclerosis. Most important, however, is the finding that the correlation of these ratios to the risk factor rating of atherosclerosis is found in subgroups with normal or elevated HDL cholesteröl values. Furthermore, it is shown that the relative content of apolipoproteinsand A-II in individual HDL is partly dependent on the plasma concentration of HDL cholesteröl and triglycerides.It is concluded that HDL composition may have an additional predictive significance for the development of atherosclerosis. HDL^Apolipoprotein A^L· und HDL-Apolipoprotein A-II-Konzentrationen bei männlichen Betriebsangehörigen in Westfalen im Alter von 40 Jahren und darüberZusammenfassung: Im Serum von 638 männlichen Betriebsangehörigen im Alter von 40 Jahren und darüber wurden die Hauptstrukturkomponenten der "high density" Lipoproteine untersucht. Es wird gezeigt, daß sowohl das HOL-Applipoprotein A-I/HDL-Cholesterin-Verhältnis als auch das HDL-Apolipoprotein A-II/ HDL-Cholesterin-Verhältnis vergleichbar mit einem kumulativen Score von bekannten Risikofaktoren der Atherosklerose korreliert ist. Am bedeutsamsten ist die Beobachtung, daß die Korrelation zwischen diesen Quotienten und dem Risiko^Score in Untergruppen mit normalen und erhöhten HDL-Cholesterinwerten erhalten bleibt. Dariiberhiüaus wird gezeigt, daß der relative Anteil von Apolipoprotein A-I und Apolipoprotein A-II in den HDL teilweise von der Konzentration von HDL-Cholesterin und Triglyceriden im Plasma abhängt.Die Ergebnisse deuten darauf hin, daß der HDL-Komposition eine zusätzliche prädiktive Bedeutung für die Entwicklung der Atherosklerose zukommt.

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Turbidimetric Assay of Apolipoprotein A-II

Cited by 3 publications

References 4 publications

Clinical chemistry

Clinical chemistry

Immunonephelometric Determination of the Apolipoprotein A-II

HDL Apolipoprotein A-I and HDL Apolipoprotein A-II Concentrations in Male Company Employees in Westphalia Aged 40 Years and Older

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