Large eddy simulation has been used to study flow in an open channel with stable stratification imposed at the free surface by a constant heat flux and an adiabatic bottom wall. This leads to a stable pycnocline overlying a well-mixed turbulent region near the bottom wall. The results are contrasted with studies in which the bottom heat flux is nonzero, a difference analogous to that between oceanic and atmospheric boundary layers. Increasing the friction Richardson number, a measure of the relative importance of the imposed surface stratification with respect to wall-generated turbulence, leads to a stronger, thicker pycnocline which eventually limits the impact of wall-generated turbulence on the free surface. Increasing stratification also leads to an increase in the pressure-driven mean streamwise velocity and a concomitant decrease in the skin friction coefficient, which is, however, smaller than in the previous channel flow studies where the bottom buoyancy flux was nonzero. It is found that the turbulence in any given region of the flow can be classified into three regimes ͑unstratified, buoyancy-affected, and buoyancy-dominated͒ based on the magnitude of the Ozmidov length scale relative to a vertical length characterizing the large scales of turbulence and to the Kolmogorov scale. Since stratification does not strongly influence the near-wall turbulent production in the present configuration, the behavior of the buoyancy flux, turbulent Prandtl number, and mixing efficiency is qualitatively different from that seen in stratified shear layers and in channel flow with fixed temperature walls, and, furthermore, collapse of quantities as a function of gradient Richardson number is not observed. The vertical Froude number is a better measure of stratified turbulence in the upper portion of the channel where buoyancy, by providing a potential energy barrier, primarily affects the transport of turbulent patches generated at the bottom wall. The characteristics of free-surface turbulence including the kinetic energy budget and pressure-strain correlations are examined and found to depend strongly on the surface stratification.