Türkiye’deki Beş Yerli Sığır Irkında Et Verim Özellikleri ile İlişkili Beş Genin Polimorfizmi

Abstract

There are many potential genes that are known to be related to meat production and that can be used for selection to improve meat quality and production include Calpain (CAPN1), Myostatin (MSTN), Calpastatin (CAST), Osteopontin (SPP1) and Thyroglobulin (TG). This study evaluated Zavot (n=60), Anatolian Black (n=59; AB), South Anatolian Red (n=53; SAR), Turkish Gray (n=60; TG) and East Anatolian Red (n=49; EAR) cattle breeds, with the primary goal of investigating DNA polymorphisms of the CAST, TG, SPP1, MSTN and CAPN1 genes. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) was employed to genotypes. Restriction enzymes revealed polymorphisms with 2 alleles and three genotypes of each CAST, TG, SPP1 and CAPN1 genes were determined in all breeds, while the MSTN locus was found to be monomorphic. Deviation from Hardy-Weinberg equilibrium (HWE) was not observed in the five cattle breeds on SPP1 locus. Significant deviation was observed from HWE in the TG and AB breeds on TG locus. Except for the TG and SAR breeds, genotype frequencies were not consistent with the HWE on CAPN1 locus. Four breeds were found at Hardy-Weinberg equilibrium except AB breed for CAST locus. Consequently, this study has shown that genetic polymorphisms do not exist in the MSTN gene but do exist in the remaining four genes that were examined in five Turkish native cattle breeds.

Boz Irk Sığırın Et Gevrekliğini Belirlemede CAST ve CAPN1 Genlerindeki Kimi SNP’lerin Kullanımı

“…The C allele frequency in our sample which is made up of purebred TGC is 0.559, which is also lower than the frequencies reported by previous researchers [20,21] . For the UoG-CAST SNP genotypes Gill et al [22] found the CC, CG and GG genotypic frequencies in Aberdeen Angus cattle as 0.41, 0.47 and 0.12, respectively.…”

“…On the other hand, Schenkel et al [5] found them as 0.430, 0.398 and 0.172, respectively for Bos taurus while Curi et al [11] found 0.377, 0.491 and 0.132, respectively for purebred Bos indicus Nellores. In our study, the ratio of the purebred TGC with CC genotype which expected to beef with more tenderness is lower than previous researches [20,21] , except Kök et al [23] . The CAPN1 316 C allele is associated with meat tenderness.…”

“…We can say that TGC with the CAPN1 4751 CT genotype (4.537±1.666 kg/cm 2 ) are more likely to have less tender meat than the Nellore breeds and Bos taurus crossbreeds [4] , Brahman calves with the CAPN1 4751 CT [3] , and also Piedmontese and Aberdeen Angus with the CAPN1 316 GG [9] . On the other hand, Gill et al [20] claim these the CAPN1 4751 and the UoG-CAST SNPs do not seem to affect meat tenderness in Aberdeen Angus cattle.…”

“…The frequency of C allele in the UoG-CAST locus is reported to be 0.74-0.79 in Bos taurus genetic groups [6] , 0.69 [11] , 0.72 [18] and 0.75 [19] in Bos taurus crossbreeds, 0.64 [20] in Aberdeen Angus cattle, 0.61 in Pirenaica cattle and 0.70 in Parda de Montana [21] , 0.623 on average in Aberdeen Angus, Limousine, Charolais and Simmental breeds [5] , 0.623 in Nellore-Bos indicus [11] . The C allele frequency in our sample which is made up of purebred TGC is 0.559, which is also lower than the frequencies reported by previous researchers [20,21] .…”

Türkiye’deki Gökçeada ve Sakız Koyun Irklarında Diacylglycerol Acyltransferase 1 (DGAT1) Gen Polimorfizminin PCR-RFLP Yöntemi İle Belirlenmesi

“…However, it has been reported that the allelic frequencies of the native breeds are lower than those of pure blood European breeds [38] . Correct usage of crossbreeding and artificial insemination techniques on native breeds in order to increase the allelic frequencies will be beneficial on the amelioration of milk quality parameters which in turn make it possible to provide the consumer with higher quality milk [39] . For example, cattle carrying the allele A of the gene ABCG2 (which codes the Breast Cancer Resistant Protein, Bcrp) have been reported to be selected since they are economically favourable [40] .…”