Turn-on theranostic fluorescent nanoprobe by electrostatic self-assembly of carbon dots with doxorubicin for targeted cancer cell imaging, in vivo hyaluronidase analysis, and targeted drug delivery

Gao, Na; Yang, Wen; Nie, Hailiang; Gong, Yunqian; Jing, Jing; Gao, Loujun; Zhang, Xiaoling

doi:10.1016/j.bios.2017.05.019

Cited by 154 publications

(72 citation statements)

References 45 publications

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“…As hypothesized, cancer cells (HeLa) were incubated with BPQDs (50 μL) at 37 °C for 3 h. Compared with confocal microscopy images of live HeLa cells not treated with BPQDs (as shown in Figure a–c), HeLa cells incubated with BPQDs present a clear blue PL emission under excitation at λ =405 nm (as shown in Figure d and e). In addition, these phenomena indicate that BPQDs possess low cytotoxicity, as well as excellent biocompatibility in HeLa cells, similar to that of carbon quantum dots, transitional‐metal dichalcogenide quantum dots, and so forth for cell bioimaging. Therefore, the obtained BPQDs, as a biocompatible material, can be utilized in high‐contrast bioimaging and biomedical applications.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Black Phosphorus Quantum Dots with High Quantum Yield by Pulsed Laser Ablation for Cell Bioimaging

Ren

Zhang

2018

Chemistry An Asian Journal

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Black phosphorus quantum dots (BPQDs), with an average diameter of about 6 nm and a height of about 1.1 nm, are successfully synthesized by means of a pulsed laser ablation (PLA) method in isopropyl ether (IPE) solvent. The photoluminescence PL quantum yield of the as-prepared sample is as high as 20.7 %, which is 3 times that of BPQDs prepared by means of probe ultrasonic exfoliation (approximately 7.2 %). The stable and blue-violet PL emission of the BPQDs is observed. It can be elucidated that electrons transit from the LUMO energy level to the HOMO energy level, as well as energy levels below the HOMO (H1 and H2). In addition, BPQDs are also utilized in bioimaging in HeLa cells, showing an intense and stable PL signal and excellent biocompatibility. Hence, this work indicates that the obtained BPQDs with high quantum yield and stable PL emission have great potential for biomedical applications, including biolabeling, bioimaging, and drug delivery.

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Section: Resultsmentioning

confidence: 99%

Synthesis of Black Phosphorus Quantum Dots with High Quantum Yield by Pulsed Laser Ablation for Cell Bioimaging

Ren

Zhang

2018

Chemistry An Asian Journal

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“…Moreover, affinity chromatography is reflected as the most useful purification method. It is based on the changeable contact between an immobilized ligand on a solid support matrix and a ligate that is a substance to be isolated from the solution, although affinity chromatography is regarded as a single‐step chromatography technique that can purify antigens in one step (Gao et al, , Raoufinia et al, ). Affinity purification is an appropriate method for the production of recombinant proteins with a purity of >90% in a single step and it is a basic purification method in the therapeutic antibody industry.…”

Section: Chromatographic Methods For Purification Of Hyalmentioning

confidence: 99%

Purification of hyaluronidase as an anticancer agent inhibiting CD44

Shakouri

Adljouy

Abdolalizadeh

2019

Biomedical Chromatography

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Hyaluronidase (Hyal) can be employed to accomplish a diversity of complications related to hyaluronic acid (HA). Hyal contains some classes of catalysts that cleave HA. This enzyme is detected in several human tissues as well as in animal venoms, pathogenic organisms and cancers. Destructive cancer cells regularly increase the CD44 receptor existing in a cell membrane. This receptor acts as an exact receptor for HA, and HA is recognized to motivate the migration, spread, attack and metastasis of cancer cells. Nearly all of the methods used to purify Hyal are highly costly and not proper for industrial applications. This survey aims to review different methods of Hyal purification, which acts as an anticancer agent by degrading HA in tissues and thus inhibiting the CD44–HA interaction. Hyal can be successfully employed in the management of cancer, which is associated with HA–CD44. This review has described different methods for Hyal purification to prepare an origin to develop a novel purification technique for this highly appreciated protein. Using multiple columns is not applicable for the purification of Hyal and thus cannot be used at the industrial level. It is better to use affinity chromatography of anti‐Hyal for Hyal with one‐step purification.

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“…The resulting FCDs exhibit a stable uorescence suitable for cell imaging application. Even more, they may be used as immune marker 21 and in bio-imaging in vivo. 22,23 Fortunately, our product can emit three different colors under three kinds of different excitation lights.…”

Section: Application As a Bio-imaging Probementioning

confidence: 99%

“…On one hand, citric acid and urea, as cheap industrial raw materials, have been well received by people with a large solubility in water. On the other hand, urea contains a large number of nitrogen and oxygen atoms 21 to ensure the generation of strong uorescence by doping or co-doping. Thus, it becomes possible to get the CDs with a high QY and a low cost in a large scale.…”

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confidence: 99%

Strongly tricolor-emitting carbon dots synthesized by a combined aging–annealing route and their bio-application

et al. 2017

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Compared with the traditional semiconductor quantum dots and organic dyes, fluorescent carbon dots (FCDs) are superior because of their high hydrophilicity, robust chemical inertness, facile functionalization, high resistance to photobleaching, low toxicity, and good biocompatibility. However, to get FCDs with a high quantum yield (QY) in a large quantity has still been challenge recently. Here we report a novel and simple method, the combined aging-annealing way, by which the CA-N-FCDs with specific characters were prepared using citric acid (CA) and urea (N source) as the raw materials. Compared with the use of a single hydrothermal treatment or annealing at relatively low temperature, the combination of the two methods can make excellent CDs. The QY of 78.8% of the as-prepared CA-N-FCDs is one of the highest yields reported till now. The optimal conditions for preparing the CA-N-FCDs were obtained including the hydrothermal aging temperature, time and the annealing conditions. The aging time was 1.5 h at around 170 C, far lower than that used in the reported annealing or calcinating processes. The brightest sample was produced by annealing at 250 C under the excitation of 365 nm light. The resulting uniform carbon nanoparticles with an average size of less than 10 nm were tricolor-emitting and blue was dominant, but including red and green colors. The CA-N-FCDs were of extremely low cell cytotoxicity applicable for being a kind of safe bio-material. Based on their high fluorescence quantum efficiency and the advantages mentioned above, these FCDs were then used for cell imaging and exhibited a perfect performance under 3 kinds of excitation bands (UV, blue, and green lights). Thus, these novel carbon dots will be able to be practically applied to immune labeling and imaging in vivo in the near future.

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Turn-on theranostic fluorescent nanoprobe by electrostatic self-assembly of carbon dots with doxorubicin for targeted cancer cell imaging, in vivo hyaluronidase analysis, and targeted drug delivery

Cited by 154 publications

References 45 publications

Synthesis of Black Phosphorus Quantum Dots with High Quantum Yield by Pulsed Laser Ablation for Cell Bioimaging

Synthesis of Black Phosphorus Quantum Dots with High Quantum Yield by Pulsed Laser Ablation for Cell Bioimaging

Purification of hyaluronidase as an anticancer agent inhibiting CD44

Strongly tricolor-emitting carbon dots synthesized by a combined aging–annealing route and their bio-application

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