Turning Meiosis into Mitosis

d’Erfurth, Isabelle; Jolivet, Sylvie; Froger, Nicole; Catrice, Olivier; Novatchkova, Maria; Mercier, Raphaël

doi:10.1371/journal.pbio.1000124

Cited by 312 publications

(298 citation statements)

References 32 publications

Supporting

Mentioning

286

Contrasting

Unclassified

Order By: Relevance

“…A virtually identical copy of the insertion has been found in every OP D. pulex tested. Moreover, abrogation of Rec8 function, which could plausibly result from the retroelement insertion, is also known in other species to result in phenotypes similar to those seen in OP D. pulex (32)(33)(34)(35)(36)(37). The finding that the Rec8 insertion and portions of three other chromosomes (8) are all strongly associated with OP is concordant with results from other systems, in which mutation of just a small number of loci is required to induce a switch from sexual to asexual reproduction.…”

Section: Discussionsupporting

confidence: 80%

“…In Arabidopsis thaliana, meiosis can be engineered to become mitosis by mutations to three different genes, including rec8, although the gametes produced by these plants were diploid and not capable of autonomous development (32). Although the causal loci in D. pulex remain to be determined, results of our previous association-mapping study (8) indicate the possibility of a comparable functional epistatic interaction, with similarly altered kinetochore orientation and homologous recombination (HR) in OP D. pulex being a result of mutation of uncharacterized loci in associated regions of chromosomes V, VIII, IX, or X (8).…”

Section: Discussionmentioning

confidence: 99%

“…Although mutations to multiple loci are required to induce obligate asexuality in A. thaliana (32,33), it is theoretically possible that Rec8 abrogation alone is sufficient to induce pseudomeiosis in D. pulex, because loss of Rec8 function affects each of the three processes modified during pseudomeiosis (HR, sisterchromatid orientation, and cohesion). Although we know of no examples of rec8 mutants that specifically result in successful clonal propagation, mutant or biochemical studies in Schizosaccharomyces pombe and other organisms (34)(35)(36)(37) are consistent with the idea that loss of Rec8 function could be sufficient to abrogate meiotic sister chromatid cohesion and HR, and to orient sister chromatids to opposite poles.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The spread of a transposon insertion in Rec8 is associated with obligate asexuality in Daphnia

Eads

Tsuchiya²,

Andrews

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Although transitions from sexual to asexual reproduction are thought to have important evolutionary consequences, little is known about the mechanistic underpinnings of these changes. The cyclical parthenogen Daphnia pulex is a powerful model in which to address these issues because female-limited meiosis suppression can be transmitted to sexual individuals via males, providing the opportunity for genetic dissection of the trait. A previous study identified genomic regions differentiating obligately asexual females from their sexual counterparts, and a candidate gene within one such region, encoding the meiotic cohesin Rec8, is the subject of this investigation. The D. pulex genome contains three Rec8 loci, all of which are quite polymorphic. However, at one of the loci, all obligately asexual clones carry an allele containing an identical upstream insertion of a transposable element as well as a frameshift mutation, both of which are completely absent from sexual lineages. The low level of variation within the insertion allele across all asexual lineages suggests that this element may be in the process of spreading through the species, and abrogation or modification of Rec8 function is possibly responsible for converting meiotically reproducing lineages into obligate asexuals.T ransitions between sexual and asexual reproduction are believed to have important evolutionary and ecological consequences (1, 2). However, the molecular, genetic, and cytological underpinnings of such transitions remain largely uncharacterized. Most asexual metazoan taxa are obligate parthenogens (3), making formal genetic analysis of these transitions impossible. In contrast, for the cyclical parthenogen Daphnia pulex, there are numerous lineages in which the females are obligately asexual, but still retain the ability to produce males capable of haploid sperm production via meiosis. Such males provide a vehicle for fertilizing the eggs of sexual females, thereby transmitting the genetic determinants of female-limited meiosis suppression (4, 5). Such sex-limited meiosis suppression provides a powerful mechanism for the recurrent transformation of sexual lineages into novel asexually produced genotypes by repeated crossings between male progeny of obligate asexuals and sexual females (5-8).Although several genetic types of obligate asexuality may exist within the Daphnia species complex (9), our current focus is on a system involving "contagious" obligate asexuality (5,7,8), which has resulted in a wide phylogenetic and geographic distribution of obligately parthenogenetic (OP) lineages in the eastern and midcontinental portions of North America.In cyclically parthenogenetic (CP) D. pulex, resting eggs are products of meiosis and fertilization, whereas, in OP females, they are produced by a pseudomeiotic process in which a germline cell undergoes an equational separation (10). Data from laboratory crosses initially suggested that a single dominant allele confers OP (7). However, a recent marker association study of a collection of OP an...

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The spread of a transposon insertion in Rec8 is associated with obligate asexuality in Daphnia

Eads

Tsuchiya²,

Andrews

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…OSD1 functions in both divisions of meiosis, and loss of its function leads to omission of the second meiotic division by itself, as well as omission of the first meiotic division hen combined with cycA1;2 (10,11). OSD1 is also involved in endoreduplication or endomitosis in cotyledons, and the loss-of-function (LoF) mutatation osd1 or gig1 (gigas cell 1) has gigantic cotyledon epidermal cells with higher ploidy (12).…”

mentioning

confidence: 99%

Perturbation of cell cycle regulation triggers plant immune response via activation of disease resistance genes

Bao

Yang

Hua

2013

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The Arabidopsis gene OSD1 (Omission of the Second Division) and its homolog UVI4 (UV-B-Insensitive 4) are negative regulators of anaphase-promoting complex/cyclosome (APC/C), a multisubunit ubiquitin E3 ligase that regulates the progression of cell cycles. Here we report the isolation of an activation tagging allele of OSD1 as an enhancer of a mutant of BON1 (BONZAI1), a negative regulator of plant immunity. Overexpression of OSD1 and UVI4 each leads to enhanced immunity to a bacterial pathogen, which is associated with increased expression of disease resistance (R) genes similar to the animal NOD1 receptor-like immune receptor genes. In addition, the reduction of function of one subunit of the APC complex APC10 exhibited a similar phenotype to that of overexpression of OSD1 or UVI4, indicating that altered APC function induces immune responses. Enhanced immune response induced by OSD1 overexpression is dependent on CYCB1;1, which is a degradation target of APC/C. It is also associated with up-regulation of R genes and is dependent on the R gene SNC1 (Suppressor of npr1-1, constitutive 1). Taken together, our findings reveal an unexpected link between cell cycle progression and plant immunity, suggesting that cell cycle misregulation could have an impact on expression of genes, including R genes, in plant immunity.

show abstract

“…For example, the genes IME1 and IME2 in budding yeast, mei2 in fission yeast, stimulated by retinoic acid 8 (Stra8) in mice and ameiotic1 (am1) in maize regulate the initiation of meiosis in different ways 3 . In A. thaliana, mutants in which female meiosis is abolished -such as dyad (also known as swi1) mutants 4,5 , spo11 rec8 osd1 triple mutants 6 or ago9 mutants that fail to silence a transposable element-derived small RNA pathway 7 produce a functional but unreduced, diploid FG. Elimination of meiosis, however, may increase ploidy of both the embryo and endosperm over successive generations, and the implication of this for crop productivity is uncertain.…”

mentioning

confidence: 99%

Developmental switches that hold the key to a revolution in crop biotechnology

Ranganath¹

2011

Nat Rev Genet

View full text Add to dashboard Cite

Turning Meiosis into Mitosis

Abstract: The mutation of as few as three genes in a sexual plant transforms meiosis into mitosis and results in diploid gametes that are genetically identical to the mother plant. This phenotype resembles apomeiosis, which is a major step in apomixis.

Cited by 312 publications

References 32 publications

The spread of a transposon insertion in Rec8 is associated with obligate asexuality in Daphnia

The spread of a transposon insertion in Rec8 is associated with obligate asexuality in Daphnia

Perturbation of cell cycle regulation triggers plant immune response via activation of disease resistance genes

Developmental switches that hold the key to a revolution in crop biotechnology

Contact Info

Product

Resources

About