Turnover of cell wall polysaccharides of a Vinca rosea suspension culture

Abstract

Three‐day‐cultured cells of Vinca rosea L. (in the cell division phase) and 5‐day‐cultured cells (in the cell expansion phase) prelabelled with d‐[U‐14C] glucose were incubated in a medium containing unlabelled glucose. After various periods of chase, extra‐cellular polysaccharides (ECP) and cell walls were isolated, and cell walls were fractionated into pectic substances, hemicellulose, and cellulose fractions. After acid hydrolysis, the radioactive constituents in the pectic substances and hemicellulose fractions were analyzed. Active turnover was observed in arabinose and galactose in the hemicellulose fraction of cell walls, while the constituents of the pectic substances, and xylose and glucose in the hemicellulose fraction did not undergo active turnover. The proportion of radioactivities of arabinose and galactose in total radioactivity of ECP increased markedly after chasing. These results indicate that arabinogalactan was synthesized, deposited in the cell wall, degraded rapidly, and made soluble in the medium as a part of ECP.

Changes in enzyme activities involved in formation and interconversion of UDP‐sugars during the cell cycle in a synchronous culture of Catharanthus roseus

“…acetates was performed on a 400 x 0,3 cm glass column packed with Chromosorb W(AW) coated with a mixture of 0,3% Reoplex 400 and 0,6% Silicone OV 275 (Mankarios et ai, 1979) at 210°C, The radio gas chromatograph was manufactured by Aloka Co,, Tokyo, The instrument comprised a Shimadzu GC-7A gas chromatograph, an Aioka F-301 sample pretreatment unit, an Aloka RGC-212 radio gas analyzer, an Aloka EDP-101 digital printer and a recorder. The principle of this system was the same as that used in Takeuchi et al, (1980) except that the internal gas flow counter system and the form of the data output were improved. The same column of 200 cm as that used in GLC was used for the separation of radioactive alditol acetates.…”

Changes in glucan synthase activities during the cell cycle in a synchronous culture of Catharanthus roseus

“…T^he radio gas chromatograph was manufactured by Aloka Co., Tokyo, and comprised a Shimadzu GC-7A gas chromatograph, an Aloka F-301 sample pretreatment unit, an Aloka RGC-212 radio gas analyzer, an Aloka EDP-101 digital printer and a recorder. The principle of this system was the satae as that used in Takeuchi et al (1980) except that the internal gas flow counter system and the form of the data output were improved. Partially methylated glucitol acetates were separated on a 200 x 0.3 cm glass column packed with Chromosorb W(AW) coated with a mixture of 0.3% (w/w) Reoplex 400 and 0.6% (w/w) Silicone OV 275 (Mankarios et al 1979) at 180°C.…”