TUSC8 inhibits the development of osteosarcoma by sponging miR‑197‑3p and targeting EHD2

Abstract

Osteosarcoma (OS) is one of the most common malignant bone tumours and generally occurs in children and adolescents. Increasing evidence has demonstrated that dysregulated long non-coding RNAs (lncRNAs) play crucial roles in the progression of various human neoplasms. Among these, tumour suppressor candidate 8 (TUSc8) is a novel lncRNA and has been reported to function as a tumour suppressor in cervical cancer. However, the exact role of TUSc8 in OS remains largely unknown. In the present study, it was observed that TUSc8 was markedly downregulated in OS tissues and cell lines. Functional experiments demonstrated that the overexpression of TUSC8 significantly suppressed the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT), whereas it accelerated the apoptosis of OS cells. Mechanistically, TUSc8 served as a sponge for miR-197-3p, and EH-domain containing 2 (EHd2) was identified as a downstream target molecule of miR-197-3p. Further investigations indicated that EHD2 knockdown significantly reversed the effects on OS cellular processes induced by TUSC8 overexpression. On the whole, these findings indicate that TUSc8 functions as a competing endogenous RNA (ceRNA) to suppress OS cell growth and EMT via the miR-197-3p/EHd2 axis. TUSc8 may thus function as a potential therapeutic target in OS treatment.

EHD2 inhibits the invasive ability of lung adenocarcinoma and improves the prognosis of patients

“…In the present study, we also used western blot analysis to identify EMT markers, and the results showed that EHD2 increased E-cadherin expression, whereas it reduced the expressions of N-cadherin and vimentin. Previous studies have indicated that EHD2 knockdown promotes EMT, while EHD2 overexpression inhibits EMT in osteosarcoma and liver cancer (13,27). Several studies have demonstrated that EMT promotes early epithelial cancer cell diffusion and is an essential parameter in epithelial cancer invasion and metastasis (28,29).…”

Hypoxia‐related lncRNAs to build prognostic classifier and reveal the immune characteristics of EGFR wild type and low expression of PD‐L1 squamous and adenocarcinoma NSCLC

“…TUSC8 and LINC01010 are reported to act as a suppressor in the invasion of multiple cancer cells in different pathways. [50][51][52][53] Multiple signatures that predict tumor prognosis also involve LINC01010. [53][54][55] AL161431.1 facilitates tumor cell proliferation and migration in endometrial carcinoma.…”

Identification of Potential Hub Genes and miRNA-mRNA Pairs Related to the Progression and Prognosis of Cervical Cancer Through Integrated Bioinformatics Analysis

“…MiRNAs generally exert their regulatory role by selectively targeting protein-coding genes. Similarly, miR-197-3p has been shown to target multiple genes in human cells, such as KLF10 ( You et al,2021 ), ABCA7( Xie et al,2020 ) and EHD2 ( Fan et al,2020 ). However, TYMS as a target of miR-197-3p in any type of cancer cell has not been studied.…”