Twelve loci from HSA10, HSA11 and HSA20 were comparatively FISH-mapped on river buffalo and sheep chromosomes

Abstract

Ten type I loci from HSA10 (IL2RA and VIM), HSA11 (HBB and FSHB) and HSA20 (THBD, AVP/OXT, GNAS1, HCK and TOP1) and two domestic cattle type II loci (CSSM30 and BL42) were FISH mapped to R-banded river buffalo (BBU) and sheep (OAR) chromosomes. IL2RA (HSA10) maps on BBU14q13 and OAR13q13, VIM (HSA10) maps on BBU14q15 and OAR13q15, HBB (HSA11) maps on BBU16q25 and OAR15q23, FSHB (HSA11) maps on BBU16q28 and OAR15q26, THBD (HSA20) maps on BBU14q15 and OAR13q15 while AVP/OXT, GNAS1, HCK, and TOP1 (HSA20) as well as CSSM30 and BL42 map on the same large band of BBU14q22 and OAR13q22. All loci were mapped on the same homologous chromosomes and chromosome bands of the two species, and these results agree with those earlier reported in cattle homologous chromosomes 15 and 13, respectively, confirming the high degree of both banding and physical map similarities among the bovid species. Indirect comparisons between physical maps achieved on bovid chromosomes and those reported on HSA10, HSA11 and HSA20 were performed.

Chromosomal localization of sixty autosomal loci in sheep (<i>Ovis aries</i>, 2n = 54) by fluorescence in situ hybridization and R-banding

“…(BM723 and D3S2 on 1p21, DVEPC119, D1S331q43 and D1S88 on 2q45, IL3 and RM006 to 5q15, D10S10 and D10S25 on 7q34, D18S21, D18S5 and MCM104 on 14q24, D20S13 and D20S10 on 16q13, D17S22 and D17S21 on 17q15, SRCRSP5 and D21S45 on 18q15, OarJMP58 and D27S6 on 26q19) compared to previous cytogenetic maps (SheepBase; Iannuzzi et al 1999Iannuzzi et al , 2000bIannuzzi et al , 2001aIannuzzi et al , 2001bDi Meo et al, 2000. Furthermore, several loci were localized on chromosome regions lacking markers (DIK26 to 1q24, CSSM147 to 2p27, McM218 to 4q15, D10S2 to 7q22, D9S5 and D9S3 to 8q14 and 8q24, respectively, OarCP09 and BM4208 to 9q22 and 9q24, respectively, BM0848 to 15q29, D20S10 to 16q13.3, D18S17 and D21S12 to 18q22, ADCY to 23q25, D27S10 to 26q15) (SheepBase; Iannuzzi et al, 1999Iannuzzi et al, , 2000bIannuzzi et al, , 2001aIannuzzi et al, , 2001bDi Meo et al, 2000. More precise localizations of molecular markers and their ordering along the chromosomes are particularly important in order to detect chromosome rearrangements that may have occurred during chromosome evolution of bovids (Piumi et al, 1998;Iannuzzi et al, 2000aIannuzzi et al, , 2001b or chromosome abnormalities, as demonstrated in cattle (Iannuzzi et al, 2001c(Iannuzzi et al, , 2001d.…”

“…With the 60 loci assigned in the present study, the total number of loci assigned to specific sheep chromosome regions amounts to 211 (SheepBase). However, it is evident that several loci earlier assigned to sheep chromosomes by FISH-mapping (Iannuzzi et al 1999(Iannuzzi et al , 2000a(Iannuzzi et al , 2000b(Iannuzzi et al , 2001a(Iannuzzi et al , 2001bDi Meo et al, 2000 have not yet been integrated in the SheepBase and an update of this database is necessary. Indeed, an improved cytogenetic map may find practical applications in clinical, evolutionary and molecular cytogenetics of sheep and related species.…”

“…Human and mouse cytogenetic maps have largely been used to extend our knowledge of specific regions of animal chromosome where conserved syntenies have been observed between bovids and humans (Womack and Moll, 1986) and later confirmed by both comparative FISH-mapping Iannuzzi et al 2000bIannuzzi et al , 2001aIannuzzi et al , 2001b and radiation hybrid mapping (Yang and Womack, 1998;Gautier et al, 2002).…”

Positional and functional characterisation of apoptosis related genes belonging to the BCL2 family in sheep

“…BCL2L1 shares the OAR13q22 band with other genes (AVP, OXT, GNAS1, HCK, TOP1) and type II markers (CSSM30, BL42) that have been FISH mapped before showing a complex evolutionary chromosome rearrangement (Iannuzzi et al, 2001a).…”

Genetic basis of the resistance to Strongyloides venezuelensis (Nematoda, Rhabdiasidae) infection in mice (Mus musculus)

“…Molecular markers associated with resistance to nematode infection in mice may contribute to mapping orthologues in ruminants because of the high chromosomal and gene similarity observed in mammals from comparative genome mapping data of ruminants, mice and humans (Lyons et al, 1997;Modi et al, 1998;O'Brien et al, 1999;Amarante et al, 2000;Band et al, 2000;Iannuzzi et al, 2001;Amarante and Amarante, 2003). It is important to remember that comparative maps have also been built to identify QTLs and eligible genes acting as a source of molecular markers for marker assisted selection (Rexroad et al, 2001).…”