Twenty-four-week oral dosing toxicities of Herba Siegesbeckiae in rats

Wu, Jia‐Ying; Chan, Yuen-Cheung; Guo, Hui; Chen, Ying‐Jie; Liu, Yuxi; Hua, Yuansheng; Yu, Zhi‐Ling

doi:10.1186/s12906-020-03137-6

Cited by 7 publications

(3 citation statements)

References 23 publications

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“…SOE treatment may cause potential side effects in animals, as discussed in several previous studies that have examined chronic toxic effects of body organs through longterm SOE treatment. Rats were treated with SOE for 24 weeks [61] or 6 months at a dose of 5 g/kg•BW/daily [62]. Following these treatments, rats had demonstrated reduced body weight gain, histopathological damage to the liver and lung, treatment-induced oxidative stress, inflammation, and MAPK activation.…”

Section: Discussionmentioning

confidence: 99%

Sigesbeckia orientalis Extract Ameliorates the Experimental Diabetic Nephropathy by Downregulating the Inflammatory and Oxidative Stress Signaling Pathways

Chen

Houng

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Diabetes in children and its complications are on the rise globally, which is accompanied by increasing in diabetes-related complications. Oxidative stress and inflammation induced by elevated blood sugar in diabetic patients are considered risk factors associated with the development of diabetes complications, including chronic kidney disease and its later development to end-stage renal disease. Microvascular changes within the kidneys of DM patients often lead to chronic kidney disease, which aggravates the illness. Sigesbeckia orientalis extract (SOE), reported to have strong antioxidative and excellent anti-inflammatory activities, is used in the modern practice of traditional Chinese medicine. Kidneys from three groups of control mice (CTR), mice with streptozotocin (STZ)-induced diabetes (DM), and mice with STZ-induced DM treated with SOE (DMRx) were excised for morphological analyses and immunohistochemical assessments. Only mice in the DM group exhibited significantly lower body weight, but higher blood sugar was present. The results revealed more obvious renal injury in the DM group than in the other groups, which appeared as greater glomerular damage and tubular injury, sores, and plenty of connective tissues within the mesangium. Not only did the DM group have a higher level of cytokine, tumor necrosis factor, and the oxidative stress marker, 8-hydroxyguanosine expression, but also factors of the nuclear factor pathway and biomarkers of microvascular status had changed. Disturbances to the kidneys in DMRx mice were attenuated compared to the DM group. We concluded that SOE is an effective medicine, with antioxidative and anti-inflammatory abilities, to protect against or attenuate diabetic nephropathy from inflammatory disturbances by oxidative stress and to cure vessel damage in a hyperglycemic situation.

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Section: Discussionmentioning

confidence: 99%

Sigesbeckia orientalis Extract Ameliorates the Experimental Diabetic Nephropathy by Downregulating the Inflammatory and Oxidative Stress Signaling Pathways

Chen

Houng

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…The liver and the kidneys were then fixed in 10% neutral buffered formalin in labeled bottles for preservation and preparation for histopathological examination. Each harvested organ’s relative weight was also calculated from formula 1 below [ 31 ]. …”

Section: Methodsmentioning

confidence: 99%

Acute and sub-acute oral toxicity of aqueous whole leaf and green rind extracts of Aloe vera in Wistar rats

Nalimu

Oloro

Peter

et al. 2022

BMC Complement Med Ther

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Background Several local communities in Central, Western, Eastern, and Northern regions of Uganda have been using the whole leaf extracts of Aloe vera (L.) Burm. f. (Asphodelaceae) in the treatment of various ailments. Also, several commercial companies sell A. vera as soft drinks in Uganda. However, there are inadequate reports on the toxicities of such preparations. This paper reports the acute and sub-acute oral toxicity of aqueous extracts of whole leaf and green rind of A. vera in Wistar rats. Methods Acute oral toxicity test was carried out in female Wistar rats at doses of 175, 550, 1750, and 5000 mg/kg, p.o. The animals were observed for signs of toxicity for 14 days. Similarly, a sub-acute oral toxicity test was performed in both sexes of rats at doses of 200, 400, and 800 mg/kg, p.o. daily for 28 days. All the groups of animals were monitored for behavioral, morphological, biochemical, and physiological changes, including mortality and compared with respective controls. Body weights were measured weekly while the animals’ relative organ weights, hematological, biochemical, gross, and microscopic pathology were examined on day 29. Results There was no mortality or apparent behavioral changes at the doses tested in acute and sub-acute oral toxicity tests. Thus, the Median Lethal Dose (LD50) of green rind and whole leaf aqueous extracts was above 5000 mg/kg. Gross anatomy revealed that the rats’ relative spleen weight in green rind extract at 200 mg/kg significantly decreased compared to the control group. The creatinine levels in female rats that received green rind extract and the chloride ion levels in male rats administered whole leaf extract were significantly elevated. Conversely, Mean Corpuscular Hemoglobin Concentration (MCHC) levels significantly decreased at lower doses of the green rind extract compared to the control. Histopathology of the kidney revealed the renal interstitium’s inflammation at doses of 200 and 800 mg/kg of the whole leaf extract. Conclusion The findings demonstrated that A. vera green rind and whole leaf extracts are non-toxic at relatively high doses when used for a short duration. Prolonged use of the aqueous whole leaf extract might be associated with kidney toxicity.

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“…After sacrificed by cervical dislocation, organs were weighed and their index in relation to body weight was calculated as described in: ROW (g) = weight of organ/bodyweight of rats on the day of sacrifice × 100% [29].…”

Section: Relative Organ Weightmentioning

confidence: 99%

Sauna Bathing Habits and Erectile Dysfunction-Tampere Ageing Male Urologic Study (TAMUS)

2022

AJUN

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Some anogenital tract malignancies have high-risk human papillomavirus (HPV) infections as their etiological cause. Although many HPV preventative vaccines have been licensed, there is still a need for medication that targets the infection and its carcinogenic effects. One of the important elements in cell immortalization and tumor development in HPV-positive cells has been identified as the viral oncoprotein E6. The cellular ubiquitin ligase E6AP interacts with E6, which can facilitate the degradation of the tumor suppressor protein p53. One of the best ways to prevent the maintenance and growth of infected cells is to block the creation of the E6-E6AP complex. The present study aims to determine the ability of polyphenols identified in Prunus armeniaca.L, to target the HPV16 virus by virtual high-throughput screening and molecular docking, and to evaluate the safety of this plant in vivo. In silico, the PDB: 4GIZ structure of E6HPV16 was prepared as a target by Discovery Studio 2021. Virtual screening of 47 polyphenols was performed by the iGEMDOCK program, followed by an evaluation of potential inhibitors based on docking affinities obtained from the The SYBYL-X Surflex-Dock module v2.0, 21. In vivo toxicity studies of Prunus armeniaca. L aqueous extract was also conducted in Wistar rats. Of all the polyphenols investigated in this study, the compounds 3-pCoumaroylquinic, 5-pCoumaroyloquinic, Epicatechin, and Dimethoxyflavone were predicted to have the highest binding affinity for E6HPV16, also revealed several interactions with the E6 binding site area. A study on acute in vivo toxicity of Prunus armeniaca .L aqueous extract was conducted and didn't produce any harmful effects. Moreover, Epicatechin, a dimethoxyflavone from Prunus armeniaca.L, 3-pCoumaroylquinic, 5-pCoumaroylquinic, and 5-pCoumaroyloquinic were chosen as possible E6HPV16 inhibitors for novel medication development.

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Twenty-four-week oral dosing toxicities of Herba Siegesbeckiae in rats

Cited by 7 publications

References 23 publications

Sigesbeckia orientalis Extract Ameliorates the Experimental Diabetic Nephropathy by Downregulating the Inflammatory and Oxidative Stress Signaling Pathways

Sigesbeckia orientalis Extract Ameliorates the Experimental Diabetic Nephropathy by Downregulating the Inflammatory and Oxidative Stress Signaling Pathways

Acute and sub-acute oral toxicity of aqueous whole leaf and green rind extracts of Aloe vera in Wistar rats

Sauna Bathing Habits and Erectile Dysfunction-Tampere Ageing Male Urologic Study (TAMUS)

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