Two Blood Group M Epitopes Disclosed by
Monoclonal Antibodies

Nichols, Margaret E.; Rosenfield, Richard E.; Rubinstein, Pablo

doi:10.1159/000466361

Cited by 6 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Monoclonal Antibodies. mAbs were TS2/18 (CD2), TS2/9 (LFA-3), TS1/22 (LFA-1) (2), control P3X63 (myeloma IgGI), E3 (human glycophorin) (22), D44 (complement receptor type 1 [CR11) (23), and IAIO (decay-accelerating factor [DAF]) (24). A goat anti-human glycophorin serum (Dr .…”

Section: Methodsmentioning

confidence: 99%

Rosetting of activated human T lymphocytes with autologous erythrocytes. Definition of the receptor and ligand molecules as CD2 and lymphocyte function-associated antigen 3 (LFA-3).

Plunkett¹,

Sanders²,

Selvaraj³

et al. 1987

The Journal of Experimental Medicine

115

View full text Add to dashboard Cite

CD2, also known as LFA-2, T11, and the E rosette receptor, is a T lymphocyte surface protein functionally important in adhesion to target cells and T cell triggering. LFA-3 is a widely distributed cell surface protein that functions in adhesion on target cells. We find that LFA-3 is expressed on human E, and that CD2 is a receptor for LFA-3 that mediates T cell adhesion to human E. Pretreatment of T lymphocytes with CD2 mAb or of E with LFA-3 mAb inhibits rosetting. Purified CD2 molecules bind to human E and inhibit rosetting. 125I-CD2 binding to E is inhibited by LFA-3 mAb; reciprocally, binding of LFA-3 mAb to human E is inhibited by pretreatment with purified CD2. Higher concentrations of CD2 aggregate human E; aggregation is inhibited by mAb to LFA-3.

show abstract

Section: Methodsmentioning

confidence: 99%

Rosetting of activated human T lymphocytes with autologous erythrocytes. Definition of the receptor and ligand molecules as CD2 and lymphocyte function-associated antigen 3 (LFA-3).

Plunkett¹,

Sanders²,

Selvaraj³

et al. 1987

The Journal of Experimental Medicine

115

View full text Add to dashboard Cite

show abstract

“…The application of hybridoma technology has resulted in the generation of monoclonal antibodies to the major sialoglycoproteins of the human red cell membrane. These proteins are of interest because they bear determinants for many blood group antigens (reviewed by Anstee, 1981;Dahr, 1981;Nichols, Rosenfield and Rubinstein, 1985). Glycophorin A or a sialoglycoprotein bears the M and N antigens, determined by the MN blood group; glycophorin B or 5 sialoglycoprotein bears antigens determined by the Ss and U genes as well as an additional N determinant known as 'N'.…”

Section: Introductionmentioning

confidence: 99%

Blood Grouping With Monoclonal Anti‐n Antibodies

Fletcher

Harbour

Matthews

et al. 1986

Aust J Exp Biol Med

View full text Add to dashboard Cite

Summary. Four monoclonal antibodies (Nl, N3, N4 and N5) with specificity for the N blood group have been produced from fusions in which human erythrocytes were used as the immunizing stimulus. Nl and N3 differed from N4 and N5 with regard to their serological specificities and pH optima. Antibody N3 has been assessed for its suitability as a blood grouping reagent by testing 10,767 blood donors in parallel with Vicia graminea anti-N lectin or rabbit anti-N. There was complete correlation between the results obtained with the monoclonal and conventional reagents providing strong evidence that N3 is a reliable and potent blood grouping reagent.

show abstract

“…Monoclonal Anti-Duffy (Anti-Fy6). Originally designated NYBC-BG6, anti-Fy6 was obtained essentially according to Kohler and Milstein (14) using a protocol described previously (15) . Briefly, pooled human red cells, used as immunogen, were injected intraperitoneally into BALB/c mice, and the spleen cells from an immunized mouse were fused with NSO/1 mouse myeloma cells .…”

mentioning

confidence: 99%

A new human Duffy blood group specificity defined by a murine monoclonal antibody. Immunogenetics and association with susceptibility to Plasmodium vivax.

Nichols¹,

Rubinstein²,

Barnwell³

et al. 1987

The Journal of Experimental Medicine

117

View full text Add to dashboard Cite

The Duffy blood group system of human erythrocytes comprises a membrane glycoprotein recently characterized by immunoprecipitation techniques (1). It is a polymorphic system that includes determinants for five alloantigens, two of which are Mendelian alleles (Fya and Fyb) (2, 3). A null genotype, designated Fy(a b-) (4), lacks the expression of both Fya and Fy b, and is inherited as a third allele of the series . Homozygous Fy(a-b-) red cells, however, also lack expression of the Fy3 (5) antigen, which is present on all other Duffy types. Fy4 (6) occurs on almost all samples of Fy(a b-), but the expression is weak on some . In addition Fy4 is occasionally found on red cells of Fy(a +b'), so that its exact Duffy status is not in perfect agreement with the absence of both Fya and Fy b (7). Fy5 (8) resembles Fy3 but is also absent from Rh-null red cells regardless of their other Duffy types.These five specificities are inherited en bloc but they differ in their susceptibility to proteolytic enzyme treatment; the high frequency antigens Fy3, Fy4, and Fy5 being resistant to the effects of chymotrypsin, ficin, and papain, while Fya and Fy b are completely destroyed by these enzymes (7). Duffy maps to chromosome 1 (9) (i.e., is syntenic to Rh), which may be related to the peculiar absence of Fy5 on Rh-null cells.Interest in the Duffy system has been considerable since the demonstration by Miller et al . (10) We have prepared a murine monoclonal antibody, anti-Fy6, that reacts with all human red cells except for those of the Fy(ab-) phenotype. The specificity defined by anti-Fy6 differs significantly from others previously identified in the Duffy system . In this report we show that Fy6 is not only characteristic of Duffy, but is related to the penetration of red cells by P. vivax merozoites. 776J. Exp. MED.

show abstract

Two Blood Group M Epitopes Disclosed by Monoclonal Antibodies

Cited by 6 publications

References 31 publications

Rosetting of activated human T lymphocytes with autologous erythrocytes. Definition of the receptor and ligand molecules as CD2 and lymphocyte function-associated antigen 3 (LFA-3).

Rosetting of activated human T lymphocytes with autologous erythrocytes. Definition of the receptor and ligand molecules as CD2 and lymphocyte function-associated antigen 3 (LFA-3).

Blood Grouping With Monoclonal Anti‐n Antibodies

A new human Duffy blood group specificity defined by a murine monoclonal antibody. Immunogenetics and association with susceptibility to Plasmodium vivax.

Contact Info

Product

Resources

About