Two Chicken B Cell Lines Resistant to Ouabain for the Production of Chicken Monoclonal Antibodies.

Nishinaka, Shigeyuki; Akiba, Hisaya; Nakamura, Mayumi; Suzuki, Kota; Suzuki, Takashi; Tsubokura, Kenji; Horiuchi, Hiroyuki; Furusawa, S; Matsuda, Haruo

doi:10.1292/jvms.58.11_1053

Cited by 41 publications

(31 citation statements)

References 17 publications

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“…Because our H-B15 chicken express Bu-1 a phenotype, antiBu-1 a antibody was used in this study. Anti-chicken IgM (4µ, mouse IgG1, k) was produced as previously described [20].…”

Section: Animalsmentioning

confidence: 99%

Development of Peyer's Patch and Cecal Tonsil in Gut-Associated Lymphoid Tissues in the Chicken Embryo

Kajiwara

Shigeta

Horiuchi

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. It is well known that chicken B cells develop in the bursa of Fabricius (BF), which is categorized as gut-associated lymphoid tissue (GALT). Chicken GALT also includes Peyer's patch (PP) and cecal tonsil (CT). The relationship between these tissues in GALT during B cell development is currently unknown. In this study, we conducted comparative examination of PP, CT and BF developmen t during embryogenesis using immunohistochemical staining. On day 13 of embryogenesis (E13), accumulation of MHC class II + cells was observed in the intestine. Thereafter, Bu-1 + cells and IgM + cells appeared, and their number continuously increased at the same sites where MHC class II + cells were present. Similar results were obtained in the CT. The locations of embryonic PP were limited to two sites; near the Meckel's diverticulum and the ileocecal junction. Anlage of bursal follicles first appeared at E13 and developed thereafter. Immigration of Bu-1 + cells to bursal follicles began at E13, and the number of Bu-1 + cell subsequently increased. When the follicle of BF was eliminated from the embryo by treatment with testosterone, development of PP and CT were observed. We concluded therefore that the development of PP and CT start during late embryogenesis at the same time as the follicle of BF, and that appearance of surface IgM + cells in PP and CT is independent form the development of the follicle of BF.KEY WORDS: bursa of Fabricius, cecal tonsil, chicken, embryo, Peyer's patch.

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“…Because our H-B15 chicken express Bu-1 a phenotype, antiBu-1 a antibody was used in this study. Anti-chicken IgM (4µ, mouse IgG1, k) was produced as previously described [20].…”

Section: Animalsmentioning

confidence: 99%

Development of Peyer's Patch and Cecal Tonsil in Gut-Associated Lymphoid Tissues in the Chicken Embryo

Kajiwara

Shigeta

Horiuchi

et al. 2003

The Journal of Veterinary Medical Science

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“…또 한 인식되어지는 항원은 sporozoite가 닭의 CD8 + T cells로의 감염에 관여하는 것으로 알려져 있으며, 이는 in vitro에서 이 항체가 Eimeria sporozoites의 CD8 + T cells로의 감염을 차 단시키는 중화 효과(neutralization effect)를 나타냄으로써 증 명되었다 (Sasai et al, 1996). 그러나 이러한 치료 목적의 닭 항체개발은 닭 하이브리도마 세포의 유전적 불안정성과 발 현되는 항체의 낮은 생산성 때문에 극히 제한되어져 있으므 로 (Nishinaka et al, 1996), 이를 극복하기 위해 항체 공학 (Winter et al, 1994)을 이용한 재조합 닭 항체를 제조하는 방법이 보고되어졌다 (Yamanaka et al, 1996).…”

Section: 서 론unclassified

“…항체 공학을 이용해 제조되는 재조합항체는 특이적 항원 결합력을 갖는 Fv 항체와 Single-chain Fv(ScFv) 항체 (Hayden et al, 1997;Verma et al, 1998) (Bird et al, 1988;Huston et al, 1991 (Nishinaka, et al, 1996) (Fig. 1 and Fig.…”

Section: 서 론unclassified

The Development of Chicken Recombinant Single-chain Fv (ScFv) Antibody Reactive with Sporozoite Antigen of Eimeria spp. which Causes Avian Coccidiosis

Park¹,

Kim

Kim³

et al. 2011

Korean Journal of Poultry Science

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The chicken monoclonal antibody (mAb), 13C8, reacts with sporozoite antigens of Eimeria spp. which causes avian coccidiosis. Since this mAb was produced at low amount due to genetic instability of chicken hybridoma, a recombinant 13C8 single-chain Fv (ScFv) antibody was constructed by amplification of the variable domain of heavy (VH) and light chain (VL) genes of antibody derived from chicken hybridoma. The constructed 13C8 ScFv was successfully expressed in E. coli and purified as a soluble form. In ELISA analysis, this recombinant 13C8 ScFv antibody showed antigen binding activity as the original mAb. In addition, nucleotide sequence comparison of 13C8 gene to the germline chicken VL and VH genes suggested that the gene conversion with Vλ and VH pseudogenes might contribute to the diversification of VL and VH genes in chickens.(

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“…The chicken B cell line, MuH1 [10], was used as the fusion partner. MuH1 cells were cultured in Iscove's modified Dulbecco's medium (IMDM) containing 10% fetal bovine serum (FBS) in a 5% CO 2 incubator at 38.5°C.…”

Section: Cell Linesmentioning

confidence: 99%

Establishment of a Chicken Monoclonal Antibody Panel Against Mammalian Prion Protein

Nakamura

Shuyama

Hojyo

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. A panel of chicken monoclonal antibodies (mAbs) was developed against prion protein (PrP), the sequence of which is a highly conserved molecule among mammals. A portion of the splenocytes from chickens immunized with recombinant mouse PrP was fused with the chicken B cell line, MuH1. The remaining splenocytes were used to generate the recombinant mAbs by phage display. A t otal of 36 anti-PrP mAbs, 2 from cell fusion and 34 from phage display were established. The specificity of these mAbs was determined by Western blot and ELISA using various PrP antigens including recombinant PrPs, synthetic PrP peptides and PrPs from brains or scr apieinfected neuroblastoma cell line. These mAbs were classified into three main groups, protease K (PK)-sensitive (Group I), PK cl eavage site proximal (Group II) and PK-resistant (Group III), based on their abilities to recognize PrP following PK-treatment. Some mAbs were found to selectively recognize different glycoforms of PrP as well as the metabolic fragments of PrP. Furthermore, we found that PrP recognition by chickens differed from that by PrP-knockout mouse. These results indicate that these newly generated PrP ant ibodies from chickens will help to research the PrP and to establish the diagnosis of prion disease. KEY WORDS: avian, panel mAb, prion protein.

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Two Chicken B Cell Lines Resistant to Ouabain for the Production of Chicken Monoclonal Antibodies.

Cited by 41 publications

References 17 publications

Development of Peyer's Patch and Cecal Tonsil in Gut-Associated Lymphoid Tissues in the Chicken Embryo

Development of Peyer's Patch and Cecal Tonsil in Gut-Associated Lymphoid Tissues in the Chicken Embryo

The Development of Chicken Recombinant Single-chain Fv (ScFv) Antibody Reactive with Sporozoite Antigen of Eimeria spp. which Causes Avian Coccidiosis

Establishment of a Chicken Monoclonal Antibody Panel Against Mammalian Prion Protein

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