Two-Color Analysis of Lymphocyte Subsets of Bronchoalveolar Lavage Fluid and Peripheral Blood in Japanese Patients With Sarcoidosis

Mukae, Hiroshi; Kohno, Shigeru; Morikawa, Toru; Kusano, Shiro; Kadota, Jun‐ichi; Hara, Kohei

doi:10.1378/chest.105.5.1474

Cited by 11 publications

(9 citation statements)

References 27 publications

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“…Previous studies have shown that CD4+ CD29+ lymphocytes in PB and BALF were significantly increased in sarcoidosis patients compared with healthy volunteers. Numbers of these cells were higher in patients with pulmonary involvement than in those without pulmonary involvement, and the numbers increased in parallel with the extent of the radiological stage 18,19 . The present study did not find a significant difference in the BAL and PB CD29 expression of the two groups; no comparison was made of CD29 expression according to radiological stage.…”

Section: Discussioncontrasting

confidence: 86%

High CD95 expression of BAL lymphocytes predicts chronic course in patients with sarcoidosis

Ozdemir¹,

Çelik²,

Dalva³

et al. 2007

Respirology

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Section: Discussioncontrasting

confidence: 86%

High CD95 expression of BAL lymphocytes predicts chronic course in patients with sarcoidosis

Ozdemir¹,

Çelik²,

Dalva³

et al. 2007

Respirology

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“…Lymphoma cells constitutively express FasL, whereas peripheral natural killer cells from healthy volunteers express FasL only when activated [7]and FasL is predominantly expressed on activated T cells, suggesting that sFasL in serum in our study probably originated from peripheral lymphocytes of patients, activated by unknown antigen. This conclusion is based on the lack of a significant correlation between serum sFasL levels and percentage of lymphocytes in BAL fluid as well as the presence of a high percentage of activated lymphocytes in peripheral blood of pulmonary sarcoidosis, as reported in our previous study [19]. Tanaka et al [15]reported that human Jurkat cells and mouse primary hepatocytes that express low levels of Fas were resistant to the soluble form of FasL in vitro and suggested that shedding of FasL serves to prevent the killing of healthy bystander cells by cytotoxic T cells.…”

Section: Discussionmentioning

confidence: 79%

High Concentrations of Soluble Fas Ligand in Bronchoalveolar Lavage Fluid of Patients with Pulmonary Sarcoidosis

Shikuwa

Kadota²,

Mukae³

et al. 2002

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Background and Objectives: Sarcoidosis is a systematic granulomatous disorder of unknown origin characterized by accumulation of T lymphocytes and macrophages in multiple organs. We postulated that apoptosis through the Fas/Fas ligand (L) system may be associated with regulation of immune reactions characterized by the formation of noncaseous necrotizing granulomas. Soluble (s) FasL is not equivalent to membrane-associated FasL since conversion of membrane-bound FasL to the soluble form is associated with downregulation of cytotoxicity. To examine the involvement of sFasL in lung inflammation, we compared the levels of sFasL in bronchoalveolar lavage (BAL) fluid and serum of patients with pulmonary sarcoidosis to those of healthy subjects. Methods: sFasL was measured in BAL fluid and in serum of 15 patients with active pulmonary sarcoidosis by sandwich ELISA. Results: High concentrations of sFasL were detected in BAL fluid and serum of patients with sarcoidosis but not in normal subjects. There was a significant correlation between the percentage of lymphocytes and sFasL concentrations in BAL fluid (r = 0.585, p < 0.05). Conclusions: Our results suggest that sFasL may be upregulated locally in the lung during the inflammatory process of active pulmonary sarcoidosis.

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“…However, the cellular content of BAL is highly variable or poorly characterized for many lung pathologies, which may be difficult to distinguish clinically from sarcoidosis [50]. Factors such as race and radiographic stage can also influence BAL findings [11,45,51], and the CD4/CD8 ratio in BAL increases with age in normal subjects [52].…”

Section: Discussionmentioning

confidence: 99%

The CD4/CD8 ratio in BAL fluid is highly variable in sarcoidosis

Kantrow¹,

Meyer²,

Kidd³

et al. 1997

Eur Respir J

131

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T Th he e C CD D4 4/ /C CD D8 8 r ra at ti io o iin n B BA AL L f fl lu ui id d i is s h hi ig gh hl ly y v va ar ri ia ab bl le e i in n s sa ar rc co oi id do os si is s S.P. Kantrow*, K.C. Meyer**, P. Kidd*, G. Raghu* The CD4/CD8 ratio in BAL fluid is highly variable in sarcoidosis. S.P. Kantrow, K.C. Meyer, P. Kidd, G. Raghu. ERS Journals Ltd 1997. ABSTRACT: Patients with pulmonary sarcoidosis frequently have increased numbers of lymphocytes and a high ratio of CD4+ to CD8+ T-lymphocytes (CD4/CD8 ratio) in bronchoalveolar lavage (BAL) fluid. Some investigators have suggested that these parameters can be used to distinguish sarcoidosis from other types of interstitial lung disease with a high degree of reliability. However, we hypothesized that the BAL CD4/CD8 ratio measured during the initial diagnostic evaluation of patients with biopsy-proven sarcoidosis is highly variable.BAL lymphocytes were analysed via flow cytometry to determine the CD4/CD8 ratio in a population of 86 patients with histological and clinical evidence of sarcoidosis, who underwent BAL as part of their initial diagnostic evaluation.In these patients, the CD4/CD8 ratio ranged 0.5-37.3, with a median value of 3.35 (mean 6.49). The CD4/CD8 ratio was greater than 4 in only 36 (42%) subjects. Ten patients (12%) had a CD4/CD8 ratio less than 1. The distribution of CD4/CD8 ratios was similar in the presence or absence of BAL lymphocytosis.In conclusion, the CD4/CD8 ratio in bronchoalveolar lavage fluid is highly variable in biopsy-proven sarcoidosis. Bronchoalveolar lavage lymphocyte subset determination is a diagnostic test with low sensitivity for this disease.

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Two-Color Analysis of Lymphocyte Subsets of Bronchoalveolar Lavage Fluid and Peripheral Blood in Japanese Patients With Sarcoidosis

Cited by 11 publications

References 27 publications

High CD95 expression of BAL lymphocytes predicts chronic course in patients with sarcoidosis

High CD95 expression of BAL lymphocytes predicts chronic course in patients with sarcoidosis

High Concentrations of Soluble Fas Ligand in Bronchoalveolar Lavage Fluid of Patients with Pulmonary Sarcoidosis

The CD4/CD8 ratio in BAL fluid is highly variable in sarcoidosis

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